Rotherhithe (/ˈrɒðərhaɪð/ RODH-ər-hydhe) is a district of South London, England, and part of the London Borough of Southwark. It is on a peninsula on the south bank of the Thames, facing Wapping, Shadwell and Limehouse on the north bank, with the Isle of Dogs to the east. It borders Bermondsey to the west and Deptford to the south-east. The district is a part of the Docklands area.

Rotherhithe has a long history as a port, with Elizabethan shipyards and working docks until the 1970s. In the 1980s, the area along the river was redeveloped as housing through a mix of warehouse conversions and new-build developments. The Jubilee line was extended to the area in 1999, giving fast connections to the West End and to Canary Wharf; the East London underground line was converted to part of the London Overground network in 2010, which provides easy access to the City of London. As a result, Rotherhithe is now a gentrifying residential and commuter area, with urban regeneration progressing around Deal Porter Square at Canada Water; a new town centre with restaurant and retail units, as well as new residential developments, is emerging here around the existing freshwater dock and transport hub.

Rotherhithe is 4.5 km (2.8 mi) east of London's centre point at Charing Cross.

The name "Rotherhithe" is thought to derive from the Anglo-Saxon Hrȳðer-hȳð, meaning "landing-place for cattle". The first recorded use of this name was in about 1105, as Rederheia. In the past Rotherhithe was also pronounced and written as Redriff(e).

The docks were closed and largely filled in during the 1980s, and have now been replaced by modern housing and commercial facilities, but Rotherhithe retains much of its character and its maritime heritage. The largest surviving dock on the south bank, Greenland Dock, is the focal point for the southern part of the district, while there are many preserved wharves along the riverside at the north end of Rotherhithe. St. Mary's Church is at the centre of the old Rotherhithe village, which contains various historic buildings including the Brunel Engine House at the south end of the Thames Tunnel.

Canada Dock was the dock basin furthest away from the River Thames in the Surrey Docks complex, and it was linked to Albion Dock and Greenland Dock at its northern and south-eastern extremities via the Albion Canal. The dock has been remodelled, and its northwest half retained as an ornamental lake, renamed Canada Water. The canal has remained as a walkway and water feature within the redeveloped area.

Southwark Council and the Greater London Authority have announced a significant regeneration project focused on Canada Water and Rotherhithe. Known as the 'Canada Water Masterplan', the project has proposed the development of an additional 3,500 homes, as well as a new high street, town square, parks, leisure centre and footpath links. The landmark Tesco store will also be relocated. The project would be developed in multiple phases over the coming 15 to 20 years. Some local community groups oppose the plan based on environmental and cost considerations.

Rotherhithe is the home of the football team Fisher F.C. and Ballers Football Academy, who train & play fixtures at St Pauls Stadium. The Championship team Millwall Football Club is nearby in the London Borough of Lewisham.