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Flammulina filiformis
Flammulina filiformis
Flammulina filiformis
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Enokitake
Cultivated Flammulina filiformis
Scientific classification Edit this classification
Kingdom: Fungi
Division: Basidiomycota
Class: Agaricomycetes
Order: Agaricales
Family: Physalacriaceae
Genus: Flammulina
Species:
F. filiformis
Binomial name
Flammulina filiformis
(Z.W. Ge, X.B. Liu & Zhu L. Yang) P.M. Wang, Y.C. Dai, E. Horak & Zhu L. Yang (2018)
Synonyms
  • Flammulina velutipes var. filiformis Z.W. Ge, X.B. Liu & Zhu L. Yang (2015)
  • Flammulina velutipes var. himalayana Z.W. Ge, Kuan Zhao & Zhu L. Yang (2015)
Flammulina filiformis
Mycological characteristics
Gills on hymenium
Cap is convex or flat
Stipe is bare
Spore print is white
Ecology is saprotrophic
Edibility is choice

Flammulina filiformis, commonly called enoki mushroom, is a species of agaric (gilled mushroom) in the family Physalacriaceae. Until recently, the species was considered to be conspecific with the European Flammulina velutipes, but DNA sequencing has shown that the two are distinct.[1]

It is widely cultivated as an edible mushroom in East Asia, where it is included in Japanese and Chinese cuisine.

Taxonomy

[edit]

Flammulina filiformis was originally described from China in 2015 as a variety of F. velutipes, based on internal transcribed spacer sequences.[2] Further molecular research using a combination of different sequences has shown that F. filiformis and F. velutipes are distinct and should be recognized as separate species.[1]

Etymology

[edit]

In Japanese, the mushroom is known as enoki-take or enoki-dake, both meaning "hackberry mushroom". This is because it is often found growing at the base of hackberry (enoki) trees.

In Mandarin Chinese, the mushroom is called jīnzhēngū (金針菇 "gold needle mushroom") or jīngū (金菇 "gold mushroom").

In Korean, it is called paengi beoseot (팽이버섯) which means "mushroom planted near catalpa". In Vietnamese it is known as nấm kim châm. In India it is called futu.

Description

[edit]

The basidiocarps are agaricoid and grow in clusters. The cap is convex at first, flattening as it expands, measuring up to 4.5 cm (1+3⁄4 in) across. The cap surface is smooth, viscid when damp, ochraceous yellow to yellow-brown.[1] The lamellae (gills) are cream to yellowish white. The stipe (stem) is up to 8.5 mm (1⁄2 in) long, smooth, pale yellow at the apex, yellow-brown to dark brown towards the base, and lacking a ring.[1] The spore print is white. The spores are inamyloid, smooth, and ellipsoid to cylindrical, measuring about 5 to 7 by 3 to 3.5 μm.[1]

There is a significant difference in appearance between wild and cultivated basidiocarps. Cultivated enokitake are not exposed to light, resulting in white or pallid fruit bodies with long stipes and small caps.

Brown variety

Distribution and habitat

[edit]

The fungus is found on dead wood of Betula platyphylla, Broussonetia papyrifera, Dipentodon sinicus, Neolitsea sp., Salix spp, and other broad-leaved trees.[1] It grows naturally in China, Korea, and Japan.

Mushrooms, enoki, raw
Nutritional value per 100 g (3.5 oz)
Energy153 kJ (37 kcal)
7.8 g
Sugars0.2 g
Dietary fiber2.7 g
0.3 g
2.7 g
Vitamins and minerals
VitaminsQuantity
%DV†
Thiamine (B1)
19%
0.23 mg
Riboflavin (B2)
15%
0.2 mg
Niacin (B3)
46%
7.3 mg
Pantothenic acid (B5)
28%
1.4 mg
Vitamin B6
6%
0.1 mg
Folate (B9)
12%
48 μg
Vitamin C
0%
0 mg
MineralsQuantity
%DV†
Calcium
0%
0 mg
Iron
7%
1.2 mg
Magnesium
4%
16 mg
Phosphorus
8%
105 mg
Potassium
12%
359 mg
Selenium
4%
2.2 μg
Sodium
0%
3 mg
Zinc
6%
0.65 mg
Other constituentsQuantity
Water88 g
Link to USDA Database entry
†Percentages estimated using US recommendations for adults,[3] except for potassium, which is estimated based on expert recommendation from the National Academies.[4]

Nutrition

[edit]

Enoki mushrooms are 88% water, 8% carbohydrates, 3% protein, and contain negligible fat (table). In a reference amount of 100 g (3.5 oz), enoki mushrooms provide 37 calories of food energy, and are a rich source (20% or more of the Daily Value, DV) of niacin (46% DV), and pantothenic acid (28% DV), while supplying moderate amounts of thiamine, riboflavin, folate, and potassium (12-19% DV, table).

Uses

[edit]

F. filiformis has been cultivated in China since 800 AD.[5] Commercial production in China was estimated at 1.57 million tonnes per annum in 2010, with Japan producing an additional 140,000 tonnes per annum.[6] The fungus can be cultivated on a range of simple lignocellulosic substrates including sawdust, wheat straw, and paddy straw.[7] Enokitake are typically grown in the dark, producing pallid fruitbodies having long and narrow stipes with undeveloped caps. Exposure to light results in more normal, short-stiped, colored fruitbodies.[7]

As food

[edit]

The mushroom is widely eaten in East Asia. Cultivated F. filiformis is sold both fresh and canned. The fungus has a crisp texture and can be refrigerated for approximately one week. It is a common ingredient for soups, especially in East Asian cuisine,[8] but can be used for salads and other dishes.

Improving storage

[edit]

F. filiformis extract can be added to whipped cream. It was observed that this measure helps to slow down the development of ice crystals, which would maintain the quality of whipped cream longer while storing it in a frozen state.[9]

Nutritionally improved meat products

[edit]

F. filiformis are an object of interest in current research for their potential to enhance food products and animal feed by using the stem waste.

Studies indicate that the addition of F. filiformis stem waste powder to meat products can improve nutritional quality by increasing dietary fiber and ash content.[10] This ingredient also enhances tenderness, inhibits lipid and protein oxidation, and extends shelf life, without negatively impacting the texture or flavor of the meat products.[11][12]

Feed additive for livestock

[edit]

Natural feed additives have become more important in livestock farming. Following this trend, F. filiformis was checked for livestock health and production efficiency improving properties. There are studies showing that the use of Enoki mushroom residue as a feed additive offers several benefits for livestock. It enhances antioxidant enzyme activity, and improves animal digestibility, hormone levels, and immunity.

The addition of mushroom residue into livestock diet can reduce the feed cost and feed conversion ratio and enhance the meat quality, providing consumers with healthier and higher-quality meat products.[13]

Cultivation

[edit]
Growing

F. filiformis is commonly cultivated in large-scale factories, where through mechanization, over 300,000 tons of F. filiformis can be harvested per year.[14]

Indoor cultivation

[edit]

F. filiformis thrives in a warm, moist environment during the incubation phase, with substrate temperatures ranging from 18 to 25 °C (64 to 77 °F). F. filiformis needs significantly cooler conditions to trigger fruiting. Pinning is triggered at temperatures ranging between 7 and 10 °C (45 and 50 °F), and the optimal temperature range for fruiting is 10 to 16 °C (50 to 61 °F). As with most fungi, F. filiformis demands elevated humidity levels—95 to 100% during pinning and 85 to 95% during fruiting.[15]

The ideal size to harvest enoki mushrooms is generally recommended to be about 5–10 cm (2–4 in) long. At that time, the cap should still be tightly closed and the stem should be long and sturdy. If people grow enoki mushrooms at home, they can use a sharp knife or scissors to snip off the mushroom cluster at the base of the stem where it meets the growing medium. It's important to remove both the mushrooms and any remaining mycelium (the white, thread-like structures) from the growing medium during harvest. This helps prevent decaying, which could negatively impact future mushroom growth.[16]

Post-harvest handling

[edit]
Packaged

F. filiformis have thin, delicate stems that need to be handled with care to prevent damage. The following steps are for reference. First, gently brush off any dirt or substrate with a soft brush or a damp cloth. After cleaning, separate the mushroom clusters into individual stems for easier cooking and presentation.[16]

Storage

[edit]

F. filiformis should be kept at temperatures between 7-10 °C (44.6-50 °F) for optimal freshness. For brief storage (fewer than 7 days), a temperature interval of 1–2 °C (34-36 °F) with 90–98% relative humidity is advised.[16]

Proneness to Listeria

[edit]

F. filiformis have the potential to be contaminated with Listeria monocytogenes, which is why disease control centers recommend cooking the mushroom prior to consumption.

The Singapore Food Agency advise people to do the following to ensure food security when consuming F. filiformis:

  1. Enoki mushrooms should never be eaten raw
  2. Instead, make sure to cook the mushrooms properly before eating them
  3. If there are cooking directions at hand, make sure to follow them
  4. Enoki mushrooms should be stored at cold temperatures to ensure a slower growth of microbes. This should be done even if the packaging is not opened yet
  5. Uncooked enoki mushroom should be stored separately to avoid cross-contamination[17]

See also

[edit]

References

[edit]
[edit]
Revisions and contributorsEdit on WikipediaRead on Wikipedia

Flammulina filiformis

View on Grokipedia
from Grokipedia
Flammulina filiformis is an edible basidiomycete belonging to the family in the order , commonly known as the winter mushroom or enoki mushroom in . In the wild, it produces yellowish-brown fruiting bodies with a bell-shaped to convex pileus up to 6 cm in diameter and a shorter stipe typically 2–10 cm tall, often with a velvety base; cultivated forms are distinctively snow-white, with elongated slender stipes up to 15–16 cm tall and small hemispherical pale caps. As a cold-adapted, wood-decaying saprotroph, it naturally inhabits decaying broadleaf wood in late autumn and winter, thriving at temperatures of 10–15°C, and is native to where it fruits from late autumn to early spring. Formerly classified under in much of the literature, F. filiformis was distinguished as a separate species through phylogenetic analyses using multi-locus markers such as ITS, tef1-α, rpb2, and HD1-A, confirming its distinct taxonomic status as the true East Asian winter mushroom, while F. velutipes is primarily European. This species is one of the most commercially important cultivated s globally, accounting for over 10% of the world's edible fungi production, with alone yielding approximately 2.025 million tons as of 2022. It is grown on lignocellulosic substrates like , cottonseed hulls, and under controlled low-temperature conditions to produce the characteristic long-stemmed form prized in . Beyond its culinary value, F. filiformis is renowned for its nutritional profile, including high levels of , , and (GABA), which contribute to its medicinal properties such as , antitumor, and antihypertensive effects. The fungus's , sequenced at 40.96 Mb with 14,256 predicted genes, has facilitated studies on its development, stress responses, and biosynthesis, highlighting conserved patterns in fruiting body formation across .

Taxonomy

Etymology

The genus name Flammulina derives from the Latin flammula, a diminutive of flamma meaning "flame," alluding to the bright orange-yellow coloration of the wild fruiting bodies that resemble small flames. The species epithet filiformis derives from the Latin filum (thread) and -formis (shaped like), referring to the filamentous suprapellis of the cap surface. Common names for Flammulina filiformis include enoki, derived from the Japanese enokitake (榎茸), combining enoki (Chinese hackberry ) and take (), reflecting its natural association with hackberry stumps; winter mushroom, due to its cold-weather fruiting; and golden needle mushroom, emphasizing the long, thin, pale appearance of cultivated specimens. This nomenclature has evolved from earlier associations with Flammulina velutipes, as genetic studies reclassified the East Asian cultivated strains as F. filiformis. In East Asian cultures, the mushroom is known as jīnzhēngū (金針菇) in , literally "gold needle ," a name highlighting its needle-like shape and golden hue in wild forms, with cultivation records dating back to around 800 AD in .

Taxonomic history and synonyms

Flammulina filiformis is a basidiomycete classified within the order and the family . Historically, the species was classified under until a 2018 phylogenetic study reclassified East Asian cultivated strains as a distinct species, F. filiformis, based on of the (ITS) and large subunit (LSU) regions, along with additional loci such as tef1-α, rpb2, and HD1-A. This integrated approach, analyzing 81 collections from , , and , recognized 12 phylogenetic species within the and highlighted genetic and morphological distinctions, with F. filiformis representing the winter mushroom (enokitake) primarily from . Synonyms for F. filiformis include Flammulina velutipes var. filiformis and the earlier binomial Collybia filiformis, reflecting its prior taxonomic placement before the 2018 delimitation. This reclassification distinguishes F. filiformis from the North American and European F. velutipes, which represents the wild, non-cultivated form with different genetic profiles and ecological adaptations. Recent genomic research, including a whole-genome sequencing effort, has further confirmed the delimitation of F. filiformis as distinct from F. velutipes, revealing a of approximately 41 Mb with 14,256 protein-coding genes and phylogenetic proximity to other basidiomycetes like . A analysis of East Asian populations has also elucidated and population structure, identifying genomic variations associated with traits such as color and stipe length, underscoring the species' evolutionary adaptations in cultivated strains. Within the genus Flammulina, which is estimated to comprise around 10 to 15 species distributed in temperate regions, F. filiformis plays a key role as the primary cultivated species in , contributing to the genus's diversity in wood-decomposing fungi.

Morphology

Wild characteristics

In its natural habitat, Flammulina filiformis produces fruiting bodies that typically grow in dense clusters on decaying substrates, exhibiting a saprotrophic . The mushrooms often emerge in late fall through winter, thriving in cold temperatures that inhibit many competing fungi. These clusters share a , with individual fruiting bodies bending near the stem base before growing upward, resulting in an overall umbrella-shaped appearance. The measures 1–6 cm in diameter, starting convex and flattening with age, often featuring an inrolled margin. It is viscid or sticky when wet, smooth, and colored orange-brown to yellowish-brown, sometimes paler toward the edges; the surface fades and the margin may become striate as the matures. The stem is 2–10 cm long and 3–10 mm thick, tough and cartilaginous, with an eccentric attachment to the ; it is white to pale yellowish above but develops a distinctive velvety, dark brown to blackish coating from the base upward. The gills are adnate to slightly , crowded, and colored white to pale yellow, with frequent short gills interspersed. The is white. Microscopically, the spores are to cylindrical, smooth, , and inamyloid, measuring 7–9 × 3–4.5 μm; pleurocystidia are present, measuring 30–60 × 8–15 μm and shaped lageniform to .

Cultivated characteristics

In commercial cultivation, Flammulina filiformis develops a characteristic form with long, thin white stems reaching up to 10 cm in length and small, hemispherical caps measuring 0.5–2 cm in diameter, often clustered tightly at the base. These features arise from growth in narrow-necked bottles or plastic bags filled with sawdust-based substrate, which constrains lateral expansion and promotes vertical elongation under low-light and high-CO2 conditions. Compared to wild specimens, cultivated F. filiformis shows reduced pigmentation, resulting in an entirely white appearance, and significantly elongated stipes due to the absence of exposure and elevated CO2 levels that inhibit development. Growth is further modified by , with primordia formation induced at 15–20°C after mycelial colonization, followed by fruiting at 5–10°C under 85–95% to yield the slender, uniform structure prized in markets. Microscopic characteristics, including spore dimensions (7–9 × 3–4.5 μm, to cylindrical, smooth, and inamyloid) and the presence of cystidia on edges, remain consistent with those of wild forms, unaffected by cultivation practices. In East Asian production centers, particularly in , varietal strains are selectively bred for enhanced yield, resistance, and morphological uniformity to meet industrial demands, with early cultivars developed in the region to standardize the pale, elongated .

Habitat and ecology

Global distribution

Flammulina filiformis is native to , encompassing regions such as southeastern , , and , where wild populations overlap with extensively cultivated strains. This species is cultivated in and , but lacks confirmed wild populations outside its native range. Globally, F. filiformis is cultivated on a large scale, dominated by East Asian producers. China leads production, yielding 2.025 million tons in 2022 and 2.3764 million tons as of 2023, followed by and as key contributors. Significant exports from these countries supply markets in the United States and , supporting international demand for this . The species exhibits a preference for temperate climates and is notably cold-adapted, favoring cooler temperatures during fruiting.

Ecological role and associations

Flammulina filiformis functions primarily as a saprotrophic decomposer in forest ecosystems, colonizing and breaking down dead and decaying hardwood substrates such as those from Populus, Salix, and Betula species. As a white-rot fungus, it secretes lignocellulolytic enzymes that degrade complex polymers like lignin and cellulose, facilitating the recycling of organic matter and nutrients back into the soil. This species exhibits notable cold tolerance, with primordia initiation and fruiting typically occurring at temperatures between 0 and 10°C, allowing it to produce fruit bodies during winter months when many other fungi are dormant. The mycelium persists through overwintering in the wood substrate, enabling renewed growth and fruiting in subsequent cold periods. In its natural , F. filiformis forms associations with bacterial communities, including genera such as and , within the rhizosphere-like zones of colonized wood; these microbes contribute to nutrient cycling by aiding in the breakdown of organic substrates and enhancing overall efficiency. Unlike many woodland fungi, F. filiformis does not engage in mycorrhizal symbioses with plants, focusing instead on saprotrophic activity. Its lignolytic capabilities also suggest potential applications in , particularly for processing wood waste through targeted degradation of recalcitrant compounds.

Nutrition and health

Nutritional composition

Flammulina filiformis, commonly known as enoki or golden needle mushroom, has a high water content of approximately 88-90% in its fresh form, contributing to its low caloric density. Per 100 grams of fresh fruiting bodies, it provides about 37 kcal, with macronutrients consisting of 2.7 g , 0.3 g fat, 7.8 g carbohydrates (including 2.7 g ), and low sodium at 3 mg. It is also rich in (GABA), approximately 20-30 mg per 100 g fresh weight. The mushroom is notable for its vitamin profile, particularly such as niacin (7 mg, or 44% of the daily value) and (0.23 mg), along with . It contains traces of vitamins C and E, and levels can increase significantly (up to 10-20 µg per 100 g) if exposed to light during cultivation or post-harvest. Key minerals include (359 mg), (105 mg), and (2.2 µg), the latter serving as an source. Additionally, F. filiformis contains unique fungal like , a sulfur-containing compound with potent properties. Nutritional variations exist between wild and cultivated forms; while macronutrients like proteins and carbohydrates show no major differences, cultivated varieties (especially white phenotypes grown in darkness) tend to have lower levels of compared to wild or yellow-capped strains, which exhibit higher -related metabolites.

Potential health benefits

Flammulina filiformis, commonly known as the enoki or golden needle mushroom, contains bioactive compounds such as (including beta-glucans and flammulin), polyphenols, and dietary fibers that contribute to its potential health benefits, primarily demonstrated in preclinical studies. These components have been investigated for their properties, which help mitigate by scavenging free radicals and enhancing endogenous antioxidant defenses. For instance, extracted from F. filiformis have shown the ability to increase (SOD) and (GSH) levels in cellular models of oxidative damage, thereby reducing and protecting against reactive oxygen species-induced injury. Polyphenols from the mushroom further support this by inhibiting oxidative processes and in animal models of . In terms of , beta-glucans in F. filiformis promote immune cell activation, including enhanced natural killer (NK) cell activity and production, which may bolster host defense mechanisms. studies have demonstrated anti-tumor potential, with extracts inhibiting proliferation of colon cancer cells through induction of and cell cycle arrest, though these effects are primarily observed at high concentrations in cell lines rather than models. effects are also notable, as F. filiformis components suppress the signaling pathway, reducing pro-inflammatory release in lipopolysaccharide-stimulated macrophages. Additionally, these compounds exhibit cardiovascular benefits by lowering serum cholesterol levels and improving profiles in hyperlipidemic animal models, potentially via binding and inhibition of absorption. Its high GABA content contributes to antihypertensive effects by promoting and reducing in animal models. Other potential benefits include neuroprotective effects, where extracts from F. filiformis have shown neuroprotective effects by enhancing neuronal survival and neurite outgrowth under conditions , promoting neuronal survival. Antidiabetic properties arise from its high content, which delays carbohydrate absorption and improves glycemic control, as evidenced by reduced blood glucose levels in diabetic mice fed F. filiformis extracts. However, human clinical trials remain limited as of 2025, with most evidence derived from and , necessitating further research to confirm efficacy and safety in s.

Culinary uses

Preparation in cuisine

Flammulina filiformis, commonly known as enoki mushroom, possesses a mild flavor profile characterized by subtle notes derived from and other , contributing to its appeal in various dishes. This taste, reminiscent of , arises from free , which also impart a slight . The mushroom's delicate, crunchy texture when fresh or lightly cooked enhances its versatility, making it a popular choice for adding contrast without overpowering other ingredients. In East Asian cuisine, F. filiformis is prominently featured in traditional dishes such as Japanese (hot pots), Chinese hot pots, and Korean , where it is often added raw to salads, simmered in soups, or incorporated into stir-fries for its crisp texture. In these preparations, the mushrooms absorb surrounding flavors while retaining a subtle crunch, commonly paired with or to balance the meal. Cooking methods typically involve brief blanching in water for 1-2 minutes to soften the stems and reduce any potential bitterness from the base, followed by minimal cooking to preserve texture and nutrients. Culturally, F. filiformis holds significant importance in , having been cultivated in since around 800 AD as both a and medicinal item, and it remains a staple in regional diets. Beyond traditional uses, the has gained traction in global , appearing as a garnish on pizzas, in stir-fries with Western ingredients, or alongside in innovative dishes, leveraging its mild taste and elongated form for visual and textural appeal. For optimal freshness in these preparations, brief storage in refrigeration is recommended before use.

Storage and preservation

Fresh Flammulina filiformis mushrooms should be stored in the at 1–4°C to maintain quality and extend , typically lasting 2–4 weeks under these conditions when kept in breathable packaging such as bags or perforated containers to prevent accumulation and excess . Exposure to ethylene-producing fruits and , such as apples or bananas, should be avoided, as ethylene accelerates cap browning and in mushrooms. Quality deterioration is indicated by visible browning of the caps or stems, which signals enzymatic oxidation and spoilage, often accompanied by weight loss exceeding 5% or off-odors from microbial activity. For longer-term preservation, F. filiformis can be dried using methods like or drying, which effectively retain flavor compounds such as eight-carbon volatiles responsible for the characteristic aroma while reducing moisture content to below 10% for indefinite storage at . Freezing is another option, but mushrooms must be blanched in water for 2–3 minutes followed by water cooling to inactivate enzymes that cause texture breakdown and flavor loss upon thawing; frozen F. filiformis maintains for up to 12 months at -18°C. in solutions of , , or a combination preserves the mushrooms through acidification and osmotic , yielding a shelf-stable product with enhanced that lasts several months in the . Advanced techniques like modified atmosphere packaging (MAP), which adjusts gas composition to low oxygen (2–5%) and high carbon dioxide (10–20%), can extend the shelf life of fresh F. filiformis to approximately 30 days at 4–5°C by slowing respiration and microbial growth. Recent 2024 research on edible coatings, such as those incorporating cinnamaldehyde nanoemulsions in alginate matrices, has shown benefits in minimizing weight loss and preserving firmness and antioxidant capacity during cold storage.

Other applications

In food processing

Flammulina filiformis serves as a valuable additive in the industry, particularly through its extracts, which enhance the texture of analogs by providing a fibrous, protein-rich structure that improves , chewiness, and overall compactness. In the production of animal-free patties, incorporating processed F. filiformis alongside proteins like and , combined with , yields products with up to 28.75 g/100 g protein content, surpassing non-mushroom formulations by approximately 45%. Polysaccharides extracted from F. filiformis function as effective fat replacers in products, such as patties and sausages, simultaneously boosting and protein while reducing fat levels. For example, full fat substitution in patties using F. filiformis polysaccharide extracts results in the lowest crude fat among tested mushroom variants, alongside elevated β-glucan levels (11.0 g/100 g ) for improved al density. Similarly, 5% addition to sausages reduces fat content and elevates free by 3.5-fold compared to controls. Enzymes and from F. filiformis contribute to storage aids in by extending through cryoprotective effects, such as inhibiting recrystallization in frozen products like , thereby preserving water-holding capacity and texture during thaw cycles at concentrations as low as 1%. Recent innovations, including 2023-filed patents for acetylated F. filiformis , enable their integration into bioactive-infused flours for baked goods like cookies, enhancing antioxidative activity and inhibiting advanced end products to improve product and .

As livestock feed

The spent substrate and mycelium from Flammulina filiformis cultivation serve as a valuable protein-rich feed for , with crude protein levels ranging from 24.1% to 30.41% on a dry basis. This byproduct enhances overall feed quality by providing essential and bioactive compounds, making it suitable for incorporation into diets for various animals. In poultry, supplementation with F. filiformis spent mushroom substrate (SMS) improves nutrient digestibility by increasing villus height and reducing crypt depth in the small intestine, thereby boosting absorption efficiency in broilers. Similarly, in pigs, fermented SMS at low inclusion levels supports intestinal health and microbiota balance, leading to better mucosal and immunity without negatively impacting activities. For ruminants, F. filiformis -based silages reduce enteric methane emissions, with trials showing decreases from 252 g/day in controls to 211 g/day in supplemented groups, attributed to phytochemicals that lower populations. This effect persists across higher inclusion levels, contributing to more sustainable animal production. Typical supplementation rates include 3% in diets, with ruminant silages often incorporating 80–90% on a basis when blended with other forages; these levels effectively repurpose cultivation waste, minimizing environmental disposal burdens. Such reduces the of farming by diverting nutrient-dense byproducts from landfills. Safety profiles are favorable, with low inherent toxin risks from edible F. filiformis strains, though monitoring for substrate-derived contaminants like is recommended to ensure feed quality. Proper processing, such as , further mitigates any potential anti-nutritional factors.

Cultivation

Production methods

Commercial cultivation of Flammulina filiformis, commonly known as enoki mushroom, primarily relies on controlled indoor environments to optimize growth and yield. The process begins with substrate preparation, where a nutrient-rich mixture serves as the base for mycelial colonization. Typical substrates consist of hardwood-derived materials such as or (around 30-35%) combined with supplements like rice bran (25-30%), wheat bran (10-25%), hulls (10-20%), and minor additives including soybean hulls, , and for adjustment to approximately 6.5-7.0. These components provide essential carbon, , and minerals while mimicking the fungus's natural wood-decaying . The mixture is adjusted to 60-65% content (substrate-to-water ratio of 1:1.6-1.7) and packed into containers, typically 900-1000 g per unit, before sterilization via autoclaving at 121°C for 1-2 hours or treatment to eliminate contaminants. Following sterilization and cooling to , the substrate is inoculated with spawn under aseptic conditions. Grain or spawn of selected F. filiformis strains is introduced at a rate of 3-5% (w/w), often 30-40 mL of spawn per , and mixed evenly to ensure uniform distribution. Incubation occurs in dark or low-light conditions using or bag systems, which are common in commercial setups for their scalability and contamination control. The incubation phase lasts 20-30 days at 18-25°C, promoting mycelial growth and full colonization of the substrate while maintaining relative humidity at 70-80% with limited fresh air exchange to keep CO₂ levels above 5,000 ppm. Fruiting is induced by shifting to cooler conditions to simulate winter environments, a critical step for primordia formation and elongated stem development characteristic of cultivated enoki. Bottles or bags are transferred to fruiting rooms at 5-10°C, with relative elevated to 85-95% to prevent , low light intensity (50-300 lx for 0.5-1 hour daily), and fresh air exchange to manage CO₂ at 7000-15,000 ppm. This phase typically spans 8-14 days, with primordia appearing at 14-16°C and 90-95% before dropping to 3-5°C for stipe elongation. Bottle and bag systems facilitate automation, including mechanized filling, inoculation, and environmental control, which are prevalent in large-scale operations. For small-scale or home cultivation using kits or blocks, general tips for success include maintaining cooler temperatures during fruiting (avoiding above 75°F or 24°C), high humidity of 85-95% achieved via tents or misting, indirect or low light to prevent browning, working in a clean space to avoid contamination such as by Trichoderma mold, a total time from start to harvest of 4-8 weeks, and expected yields of 100-500 g per kit or block. Strains are selectively bred or genetically optimized for superior traits in commercial production. High-yield cultivars, such as those derived from monokaryon selections like Fv-HL23 or recent strains like FfWRY-18, achieve biological efficiencies of 100-150% (fresh weight yield relative to dry substrate weight), with outputs up to 350-400 g per bottle under optimized conditions, including recent advancements in soybean straw-based substrates yielding up to 122.73% efficiency. In , the dominant producer, automation in factory-style farms has driven output to exceed 2.1 million tons in 2023. Outdoor cultivation or wild simulation methods are rare and limited to small-scale or specialty markets, often involving inoculation of hardwood logs in shaded, cool climates (below 15°C) to mimic natural growth on deciduous trees. These approaches yield lower biological efficiencies (under 50%) and are not viable for commercial volumes due to inconsistent environmental control and contamination risks.

Harvesting and handling

Flammulina filiformis, commonly known as enoki mushroom, is typically harvested after a cultivation cycle of 30 to 60 days from inoculation, depending on environmental conditions and strain. Harvesting occurs when stems reach 8-12 cm in length, ensuring optimal texture and market appeal before the caps fully expand or stems become fibrous. The mushrooms are collected by gently twisting or cutting the clusters at the base with a clean, sharp tool to minimize damage to the substrate and preserve cluster integrity. Post-harvest processing begins immediately to maintain . Mushrooms undergo minimal with cool to remove without introducing excess that could accelerate spoilage, followed by trimming of the root-like bases for cleanliness and uniformity. They are then sorted by size and to ensure consistent , with yields typically ranging from 200-300 g of fresh mushrooms per standard cultivation bottle. In large-scale operations, mechanized sorting and trimming equipment is employed to handle high volumes efficiently while reducing labor-intensive manual processes. Packaging involves placing the trimmed clusters into (PP) bags or breathable films to allow and prevent buildup. A is essential throughout transport and initial storage, maintaining temperatures of 0-4°C to inhibit enzymatic browning and microbial growth. Mechanical damage during handling, such as bruising from rough transport, accounts for 10-15% of post-harvest losses, emphasizing the need for gentle manipulation.

Safety concerns

Listeria contamination

Listeria monocytogenes contamination is prevalent in Flammulina filiformis (enoki mushrooms) due to the crop's cultivation in cool, moist environments that favor bacterial survival and growth. Studies have shown that contamination levels at harvest can average around 10^3 colony-forming units per gram (cfu/g), with variability depending on production conditions. In imported enoki samples, prevalence rates range from 15% to 46.9%, with higher rates observed in products from major exporters like China and Korea; for instance, 43% of Korean imports tested positive in U.S. surveys. Between 2024 and 2025, multiple U.S. FDA alerts and recalls targeted imported brands, including nationwide actions in April, September, October, November, and December 2024, as well as March, June, and September 2025, often linked to routine sampling detecting the pathogen. Sources of contamination include farm environments such as soil and irrigation water, where can persist, as well as post-harvest cross-contamination during handling and packaging. Bacterial studies indicate that interactions between and species, common in cultivation, can enhance Listeria's survival; for example, biofilms provide protective niches that increase Listeria's tolerance to sanitizers and allow co-colonization on surfaces. Contamination often enters during the growing phase in high-humidity, CO2-enriched cultivation bags, enabling the to proliferate alongside the mushrooms. Control measures emphasize Hazard Analysis and Critical Control Points (HACCP)-like protocols in production, including strict sanitation in growing facilities and temperature management to limit growth, with refrigeration at 1–4°C recommended to halt proliferation. Post-harvest interventions include chlorine-based washes and, in some cases, irradiation to reduce bacterial loads, though efficacy varies with biofilm presence. Recent 2025 research highlights the role of biofilms in cultivation bags, showing that Listeria can embed within multi-species communities, necessitating targeted disinfectants and regular bag sterilization to disrupt these structures. FDA strategies also promote international training on good manufacturing practices and increased import sampling to prevent contaminated products from reaching markets, with recalls continuing as of November 2025. Outbreaks linked to ready-to-eat enoki mushrooms have disproportionately affected high-risk groups, including pregnant individuals, newborns, the elderly, and immunocompromised people, with carrying a case-fatality rate of 15–30%. Notable incidents include the 2020 U.S.- outbreak with 36 cases and 4 deaths (11% fatality), traced to Korean imports, and a 2022 U.S. outbreak involving 6 cases, all hospitalized, linked to enoki mushrooms imported from . These events underscore the pathogen's ability to grow under , amplifying risks in minimally processed products. No new outbreaks have been reported as of November 2025, though recalls persist.

Other risks

Flammulina filiformis is generally regarded as an with no inherent , lacking dangerous compounds such as . However, rare allergic reactions, including urticaria and , have been documented following its consumption, particularly in sensitized individuals. foraged specimens pose a misidentification risk with toxic lookalikes like Galerina species, which contain and can lead to fatal liver damage if consumed. Enoki mushrooms from clean sources are safe for consumption when properly handled and cooked to mitigate risks such as Listeria contamination, but wild specimens should only be eaten after expert identification to avoid these toxic lookalikes. As with many fungi, F. filiformis can bioaccumulate such as from contaminated substrates, especially in polluted cultivation areas, potentially leading to chronic risks upon repeated exposure. residues have also been identified in imported enoki mushrooms, raising concerns for consumer safety in . Raw consumption of F. filiformis may result in mild gastrointestinal upset, attributable to the indigestible comprising its cell walls, which resists human enzymatic breakdown. Chitin is a white amorphous solid and a type of dietary fiber, also the primary component of the exoskeletons of shrimp, crabs, and other arthropods. It is insoluble in water, alkaline solutions, and organic solvents, but soluble only in strong acids. This contributes to why raw enoki mushrooms may pass through the digestive system largely intact. Cooking mitigates this issue by partially degrading the structure. Regulatory measures address these hazards; the US FDA maintains import alerts detaining untreated enoki mushrooms from high-risk countries like and as of 2025, primarily to curb contamination but encompassing broader safety evaluations. Recent 2025 studies highlight how , through altered soil pH and moisture, may enhance heavy metal uptake in mushrooms like F. filiformis, amplifying environmental contaminant risks.

References

  1. https://pmc.ncbi.nlm.nih.gov/articles/PMC11678480/
  2. https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.887259/full
  3. https://pmc.ncbi.nlm.nih.gov/articles/PMC7567395/
  4. https://link.springer.com/article/10.1007/s11557-018-1409-2
  5. https://www.first-nature.com/fungi/flammulina-elastica.php
  6. https://www.mycobank.org/page/Name%20details%20page/field/Mycobank%20%23/819132
  7. https://en.wiktionary.org/wiki/enoki_mushroom
  8. https://pmc.ncbi.nlm.nih.gov/articles/PMC10056640/
  9. https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2016.00474/full
  10. https://www.researchgate.net/publication/325826280_Phylogeny_and_species_delimitation_of_Flammulina_taxonomic_status_of_winter_mushroom_in_East_Asia_and_a_new_European_species_identified_using_an_integrated_approach
  11. https://www.sciencedirect.com/science/article/pii/S2090123224004971
  12. https://www.cabidigitallibrary.org/doi/full/10.1079/cabicompendium.13982
  13. https://minds.wisconsin.edu/bitstream/handle/1793/83597/Roehl_Thomas_Thesis.pdf?sequence=1&isAllowed=y
  14. https://www.mdpi.com/2311-7524/11/8/947
  15. https://www.mdpi.com/2309-608X/9/3/330
  16. https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-025-11555-4
  17. https://www.mycosphere.org/pdf/MYCOSPHERE_16_1_17.pdf
  18. https://www.mdpi.com/2309-608X/11/3/229
  19. https://www.alliedmarketresearch.com/enoki-mushroom-market-A35096
  20. https://www.mdpi.com/2311-7524/11/8/912
  21. https://pmc.ncbi.nlm.nih.gov/articles/PMC10424591/
  22. https://www.gbif.org/species/144093990
  23. https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-07108-6
  24. https://www.jstage.jst.go.jp/article/bio/21/3/21_153/_article
  25. https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1429213/full
  26. https://foodstruct.com/food/enoki-mushrooms
  27. https://www.megawecare.com/good-health-by-yourself/nutrition/enoki-mushrooms-benefits
  28. https://www.nutrition-and-you.com/enoki-mushroom.html
  29. https://www.healthline.com/nutrition/enoki-mushrooms-benefits
  30. https://www.webmd.com/diet/health-benefits-of-enoki-mushrooms
  31. https://pmc.ncbi.nlm.nih.gov/articles/PMC5141589/
  32. https://www.mdpi.com/2309-608X/9/11/1063
  33. https://pmc.ncbi.nlm.nih.gov/articles/PMC9500316/
  34. https://www.sciencedirect.com/science/article/pii/S0144861724002042
  35. https://www.jstage.jst.go.jp/article/fstr/19/4/19_661/_pdf
  36. https://www.sciencedirect.com/science/article/abs/pii/S2212429221003710
  37. https://pmc.ncbi.nlm.nih.gov/articles/PMC12526165/
  38. https://pmc.ncbi.nlm.nih.gov/articles/PMC9794872/
  39. https://pmc.ncbi.nlm.nih.gov/articles/PMC9864181/
  40. https://extension.psu.edu/preparing-and-preserving-mushrooms/
  41. https://cargohandbook.com/Mushrooms
  42. https://pdfs.semanticscholar.org/1967/27c5e86e36037e985c74ef687232db826c7e.pdf
  43. https://pmc.ncbi.nlm.nih.gov/articles/PMC10060591/
  44. https://nchfp.uga.edu/how/freeze/vegetable/freezing-mushrooms/
  45. https://foragerchef.com/fish-sauce-pickled-enoki/
  46. https://www.thespruceeats.com/quick-soy-sauce-pickled-shiitake-mushrooms-recipe-7557000
  47. https://www.foodstandards.gov.au/sites/default/files/2025-07/Lmono-on-enoki-mushroom.pdf
  48. https://pmc.ncbi.nlm.nih.gov/articles/PMC11120273/
  49. https://www.mdpi.com/2079-6412/14/2/172
  50. https://doi.org/10.1515/ijfe-2022-0204
  51. https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2023.1111955/full
  52. https://doi.org/10.1016/j.meatsci.2019.04.003
  53. https://doi.org/10.1016/j.jfoodeng.2025.112487
  54. https://patents.google.com/patent/CN119661730A/en
  55. https://www.researchgate.net/figure/Spent-mushroom-substrate-SMS-used-as-an-animal-feedstock_tbl2_377166204
  56. https://www.tandfonline.com/doi/full/10.1080/21655979.2023.2252138
  57. https://pmc.ncbi.nlm.nih.gov/articles/PMC11590908/
  58. https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2020.584243/full
  59. https://www.sciencedirect.com/science/article/abs/pii/S0377840117303322
  60. https://pmc.ncbi.nlm.nih.gov/articles/PMC6132538/
  61. https://www.scirp.org/journal/paperinformation?paperid=80257
  62. https://grocycle.com/enoki-mushrooms/
  63. https://northspore.com/blogs/the-black-trumpet/how-to-grow-and-cook-golden-enoki-mushrooms-at-home
  64. https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.00498/full
  65. https://www.mdpi.com/2077-0472/15/10/1045
  66. https://doi.org/10.3390/agronomy15020432
  67. https://www.mushroomsextracts.com/news/How-do-enoki-mushrooms-grow-id40693326/
  68. https://www.slideshare.net/slideshow/enoki-mushroom-and-its-cultivation/264903682
  69. https://freshfoodpacking.net/xh/the-ultimate-guide-how-to-grow-enoki-mushrooms-and-package-them-for-market/
  70. https://www.researchgate.net/publication/373537165_Enokitake_Flammulina_velutipes
  71. https://www.sciencedirect.com/science/article/pii/S259015752400662X
  72. https://www.scribd.com/document/848850694/Enoki-PDF-Avick
  73. https://www.researchgate.net/publication/262725706_POST_HARVEST_TECHNOLOGY_OF_MUSHROOMS_Technical_Bulletin
  74. https://www.sciencedirect.com/science/article/pii/S0362028X24000747
  75. https://science.food.gov.uk/article/122719-rapid-risk-assessment-of-the-detection-of-listeria-monocytogenes-in-enoki-mushrooms
  76. https://www.sciencedirect.com/science/article/abs/pii/S0740002024002259
  77. https://www.fda.gov/food/new-era-smarter-food-safety/summary-fdas-strategy-help-prevent-listeriosis-and-salmonellosis-outbreaks-associated-imported-enoki
  78. https://www.sciencedirect.com/science/article/abs/pii/S0963996925016059
  79. https://www.foodsafetynews.com/2025/11/enoki-mushrooms-recalled-in-canada-for-contamination-with-listeria/
  80. https://pmc.ncbi.nlm.nih.gov/articles/PMC12441183/
  81. https://www.cdc.gov/listeria/outbreaks/enoki-11-22/index.html
  82. https://pubmed.ncbi.nlm.nih.gov/29249758/
  83. https://www.semanticscholar.org/paper/A-case-of-anaphylaxis-after-ingestion-of-Enokitake-Minami-Kanzaki/9610abf3328b69d2ae4a4c6dbd6c7b627e4acb53
  84. https://www.mushroom-appreciation.com/the-two-enoki-mushrooms.html
  85. https://ohiomushroomfestival.com/2024/11/24/flammulina-velutipes-the-velvet-foot-or-enoki/
  86. https://pmc.ncbi.nlm.nih.gov/articles/PMC8778862/
  87. https://www.foodsafetynews.com/2025/01/pesticide-residues-dominate-eu-import-control-changes/
  88. https://fungi.com/blogs/articles/should-you-consume-raw-mushrooms
  89. https://www.css.ch/en/private-customers/my-health/nutrition/nutrition-knowledge/digesting-mushrooms.html
  90. https://www.britannica.com/science/chitin
  91. https://pmc.ncbi.nlm.nih.gov/articles/PMC8961438/
  92. https://www.accessdata.fda.gov/cms_ia/importalert_1177.html
  93. https://link.springer.com/article/10.1007/s43994-025-00241-6
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