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Brewing
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Brewing is the production of beer by steeping a starch source (commonly cereal grains, the most popular of which is barley)[1] in water and fermenting the resulting sweet liquid with yeast. It may be done in a brewery by a commercial brewer, at home by a homebrewer, or communally.[2] Brewing has taken place since around the 6th millennium BC, and archaeological evidence suggests that emerging civilizations, including ancient Egypt,[3] China,[4] and Mesopotamia, brewed beer.[5] Since the nineteenth century the brewing industry has been part of most western economies.
The basic ingredients of beer are water and a fermentable starch source such as malted barley. Most beer is fermented with a brewer's yeast and flavoured with hops.[6] Less widely used starch sources include millet, sorghum and cassava.[7] Secondary sources (adjuncts), such as maize (corn), rice, or sugar, may also be used, sometimes to reduce cost, or to add a feature, such as adding wheat to aid in retaining the foamy head of the beer.[8] The most common starch source is ground cereal or "grist" – the proportion of the starch or cereal ingredients in a beer recipe may be called grist, grain bill, or simply mash ingredients.[9]
Steps in the brewing process include malting, milling, mashing, lautering, boiling, fermenting, conditioning, filtering, and packaging. There are three main fermentation methods: warm, cool and spontaneous. Fermentation may take place in an open or closed fermenting vessel; a secondary fermentation may also occur in the cask or bottle. There are several additional brewing methods, such as Burtonisation, double dropping, and Yorkshire Square, as well as post-fermentation treatment such as filtering, and barrel-ageing.
History
[edit]
Brewing has taken place since around the 6th millennium BC, and archaeological evidence suggests emerging civilizations including China,[4] ancient Egypt, and Mesopotamia brewed beer. Descriptions of various beer recipes can be found in cuneiform (the oldest known writing) from ancient Mesopotamia.[3][11][12] In Mesopotamia the brewer's craft was the only profession which derived social sanction and divine protection from female deities/goddesses, specifically: Ninkasi, who covered the production of beer, Siris, who was used in a metonymic way to refer to beer, and Siduri, who covered the enjoyment of beer.[5] In pre-industrial times, and in developing countries, women are frequently the main brewers.[13][14]
As almost any cereal containing certain sugars can undergo spontaneous fermentation due to wild yeasts in the air, it is possible that beer-like beverages were independently developed throughout the world soon after a tribe or culture had domesticated cereal. Chemical tests of ancient pottery jars reveal that beer was produced as far back as about 7,000 years ago in what is today Iran. This discovery reveals one of the earliest known uses of fermentation and is the earliest evidence of brewing to date. In Mesopotamia, the oldest evidence of beer is believed to be a 6,000-year-old Sumerian tablet depicting people drinking a beverage through reed straws from a communal bowl. A 3900-year-old Sumerian poem honouring Ninkasi, the patron goddess of brewing, contains the oldest surviving beer recipe, describing the production of beer from barley via bread. The invention of bread and beer has been argued to be responsible for humanity's ability to develop technology and build civilization.[15][16][17] The earliest chemically confirmed barley beer to date was discovered at Godin Tepe in the central Zagros Mountains of Iran, where fragments of a jug, at least 5,000 years old was found to be coated with beerstone, a by-product of the brewing process.[18] Beer may have been known in Neolithic Europe as far back as 5,000 years ago,[19] and was mainly brewed on a domestic scale.[20]
Ale produced before the Industrial Revolution continued to be made and sold on a domestic scale, although by the 7th century AD beer was also being produced and sold by European monasteries. During the Industrial Revolution, the production of beer moved from artisanal manufacture to industrial manufacture, and domestic manufacture ceased to be significant by the end of the 19th century.[21] The development of hydrometers and thermometers changed brewing by allowing the brewer more control of the process, and greater knowledge of the results. Today, the brewing industry is a global business, consisting of several dominant multinational companies and many thousands of smaller producers ranging from brewpubs to regional breweries.[22] More than 133 billion litres (35 billion gallons) are sold per year—producing total global revenues of $294.5 billion (£147.7 billion) in 2006.[23]
Ingredients
[edit]
The basic ingredients of beer are water; a starch source, such as malted barley, able to be fermented (converted into alcohol); a brewer's yeast to produce the fermentation; and a flavouring, such as hops,[6] to offset the sweetness of the malt.[24] A mixture of starch sources may be used, with a secondary saccharide, such as maize (corn), rice, or sugar, these often being termed adjuncts, especially when used as a lower-cost substitute for malted barley.[8] Less widely used starch sources include millet, sorghum, and cassava root in Africa, potato in Brazil, and agave in Mexico, among others.[7] The most common starch source is ground cereal or "grist" – the proportion of the starch or cereal ingredients in a beer recipe may be called grist, grain bill, or simply mash ingredients.[9]
- Water
Beer is composed mostly of water. Regions have water with different mineral components; as a result, different regions were originally better suited to making certain types of beer, thus giving them a regional character.[25][26] For example, Dublin has hard water well suited to making stout, such as Guinness; while Pilsen has soft water well suited to making pale lager, such as Pilsner Urquell.[25] The waters of Burton in England contain gypsum, which benefits making pale ale to such a degree that brewers of pale ales will add gypsum to the local water in a process known as Burtonisation.[27]
- Starch source
The starch source in a beer provides the fermentable material and is a key determinant of the strength and flavour of the beer. The most common starch source used in beer is malted grain. Grain is malted by soaking it in water, allowing it to begin germination, and then drying the partially germinated grain in a kiln. Malting grain produces enzymes that will allow conversion from starches in the grain into fermentable sugars during the mash process.[28] Different roasting times and temperatures are used to produce different colours of malt from the same grain. Darker malts will produce darker beers.[29]
Nearly all beer includes barley malt as the majority of the starch. This is because of its fibrous husk, which is important not only in the sparging stage of brewing (in which water is washed over the mashed barley grains to form the wort) but also as a rich source of amylase, a digestive enzyme that facilitates conversion of starch into sugars. Other malted and unmalted grains (including wheat, rice, oats, and rye, and, less frequently, maize (corn) and sorghum) may be used. In recent years, a few brewers have produced gluten-free beer made with sorghum with no barley malt for people who cannot digest gluten-containing grains like wheat, barley, and rye.[30]
- Hops

Hops are the female flower clusters or seed cones of the hop vine Humulus lupulus,[31] which are used as a flavouring and preservative agent in nearly all beer made today.[32] Hops had been used for medicinal and food flavouring purposes since Roman times; by the 7th century in Carolingian monasteries in what is now Germany, beer was being made with hops,[33] though it isn't until the thirteenth century that widespread cultivation of hops for use in beer is recorded.[34] Before the thirteenth century, beer was flavoured with plants such as yarrow, wild rosemary, and bog myrtle, and other ingredients such as juniper berries, aniseed and ginger, which would be combined into a mixture known as gruit and used as hops are now used; between the thirteenth and the sixteenth century, during which hops took over as the dominant flavouring, beer flavoured with gruit was known as ale, while beer flavoured with hops was known as beer.[35][36] Some beers today, such as Fraoch by the Scottish Heather Ales company and Cervoise Lancelot by the French Brasserie-Lancelot company, use plants other than hops for flavouring.[37][38]
Hops contain several characteristics that brewers desire in beer: they contribute a bitterness that balances the sweetness of the malt; they provide floral, citrus, and herbal aromas and flavours; they have an antibiotic effect that favours the activity of brewer's yeast over less desirable microorganisms; and they aid in "head retention", the length of time that the foam on top of the beer (the beer head) will last.[39] The preservative in hops comes from the lupulin glands which contain soft resins with alpha and beta acids.[40][41] Though much studied, the preservative nature of the soft resins is not yet fully understood, though it has been observed that unless stored at a cool temperature, the preservative nature will decrease.[42][43] Brewing is the sole major commercial use of hops.[44]
- Yeast
Yeast is the microorganism that is responsible for fermentation in beer. Yeast metabolises the sugars extracted from grains, which produces alcohol and carbon dioxide, and thereby turns wort into beer. In addition to fermenting the beer, yeast influences the character and flavour.[45] The dominant types of yeast used to make beer are Saccharomyces cerevisiae, known as ale yeast, and Saccharomyces pastorianus, known as lager yeast; Brettanomyces ferments lambics,[46] and Torulaspora delbrueckii ferments Bavarian weissbier.[47] Before the role of yeast in fermentation was understood, fermentation involved wild or airborne yeasts, and a few styles such as lambics still use this method today. Emil Christian Hansen, a Danish biochemist employed by the Carlsberg Laboratory, developed pure yeast cultures which were introduced into the Carlsberg brewery in 1883,[48] and pure yeast strains are now the main fermenting source used worldwide.[49]
- Clarifying agent
Some brewers add one or more clarifying agents to beer, which typically precipitate (collect as a solid) out of the beer along with protein solids and are found only in trace amounts in the finished product. This process makes the beer appear bright and clean, rather than the cloudy appearance of ethnic and older styles of beer such as wheat beers.[50]
Examples of clarifying agents include isinglass, obtained from swim bladders of fish; Irish moss, a seaweed; kappa carrageenan, from the seaweed kappaphycus; polyclar (a commercial brand of clarifier); and gelatin.[51] If a beer is marked "suitable for Vegans", it was generally clarified either with seaweed or with artificial agents,[52] although the "Fast Cask" method invented by Marston's in 2009 may provide another method.[53]
Brewing process
[edit]There are several steps in the brewing process, which may include malting, mashing, lautering, boiling, fermenting, conditioning, filtering, and packaging.[54] The brewing equipment needed to make beer has grown more sophisticated over time, and now covers most aspects of the brewing process.[55][56]
Malting is the process where barley grain is made ready for brewing.[57] Malting is broken down into three steps in order to help to release the starches in the barley.[58] First, during steeping, the grain is added to a vat with water and allowed to soak for approximately 40 hours.[59] During germination, the grain is spread out on the floor of the germination room for around 5 days.[59] The final part of malting is kilning when the malt goes through a very high temperature drying in a kiln; with gradual temperature increase over several hours.[60] When kilning is complete, the grains are now termed malt, and they will be milled or crushed to break apart the kernels and expose the cotyledon, which contains the majority of the carbohydrates and sugars; this makes it easier to extract the sugars during mashing.[61]
Mashing converts the starches released during the malting stage into sugars that can be fermented. The milled grain is mixed with hot water in a large vessel known as a mash tun. In this vessel, the grain and water are mixed together to create a cereal mash. During the mash, naturally occurring enzymes present in the malt convert the starches (long chain carbohydrates) in the grain into smaller molecules or simple sugars (mono-, di-, and tri-saccharides). This "conversion" is called saccharification which occurs between the temperatures 60–70 °C (140–158 °F).[62] The result of the mashing process is a sugar-rich liquid or "wort", which is then strained through the bottom of the mash tun in a process known as lautering. Prior to lautering, the mash temperature may be raised to about 75–78 °C (167–172 °F) (known as a mashout) to free up more starch and reduce mash viscosity. Additional water may be sprinkled on the grains to extract additional sugars (a process known as sparging).[63]
The wort is moved into a large tank known as a "copper" or kettle where it is boiled with hops and sometimes other ingredients such as herbs or sugars. This stage is where many chemical reactions take place, and where important decisions about the flavour, colour, and aroma of the beer are made.[64] The boiling process serves to terminate enzymatic processes, precipitate proteins, isomerize hop resins, and concentrate and sterilize the wort. Hops add flavour, aroma and bitterness to the beer. At the end of the boil, the hopped wort settles to clarify in a vessel called a "whirlpool", where the more solid particles in the wort are separated out.[65]
After the whirlpool, the wort is drawn away from the compacted hop trub, and rapidly cooled via a heat exchanger to a temperature where yeast can be added. A variety of heat exchanger designs are used in breweries, with the most common a plate-style. Water or glycol run in channels in the opposite direction of the wort, causing a rapid drop in temperature. It is very important to quickly cool the wort to a level where yeast can be added safely as yeast is unable to grow in very high temperatures, and will start to die in temperatures above 60 °C (140 °F).[61][66] After the wort goes through the heat exchanger, the cooled wort goes into a fermentation tank. A type of yeast is selected and added, or "pitched", to the fermentation tank.[64] When the yeast is added to the wort, the fermenting process begins, where the sugars turn into alcohol, carbon dioxide and other components. When the fermentation is complete the brewer may rack the beer into a new tank, called a conditioning tank.[63] Conditioning of the beer is the process in which the beer ages, the flavour becomes smoother, and flavours that are unwanted dissipate.[65] After conditioning for a week to several months, the beer may be filtered and force carbonated for bottling,[67] or fined in the cask.[68]
Mashing
[edit]
Mashing is the process of combining a mix of milled grain (typically malted barley with supplementary grains such as corn, sorghum, rye or wheat), known as the "grist" or "grain bill", and water, known as "liquor", and heating this mixture in a vessel called a "mash tun". Mashing is a form of steeping,[69] and defines the act of brewing, such as with making tea, sake, and soy sauce.[70] Technically, wine, cider and mead are not brewed but rather vinified, as there is no steeping process involving solids.[71] Mashing allows the enzymes in the malt to break down the starch in the grain into sugars, typically maltose to create a malty liquid called wort.[72] There are two main methods – infusion mashing, in which the grains are heated in one vessel; and decoction mashing, in which a proportion of the grains are boiled and then returned to the mash, raising the temperature.[73] Mashing involves pauses at certain temperatures (notably 45–62–73 °C or 113–144–163 °F), and takes place in a "mash tun" – an insulated brewing vessel with a false bottom.[74][75][76] The end product of mashing is called a "mash".
Mashing usually takes 1 to 2 hours, and during this time the various temperature rests activate different enzymes depending upon the type of malt being used, its modification level, and the intention of the brewer. The activity of these enzymes convert the starches of the grains to dextrins and then to fermentable sugars such as maltose. A mash rest from 49–55 °C (120–131 °F) activates various proteases, which break down proteins that might otherwise cause the beer to be hazy. This rest is generally used only with undermodified (i.e. undermalted) malts which are decreasingly popular in Germany and the Czech Republic, or non-malted grains such as corn and rice, which are widely used in North American beers. A mash rest at 60 °C (140 °F) activates β-glucanase, which breaks down gummy β-glucans in the mash, making the sugars flow out more freely later in the process. In the modern mashing process, commercial fungal based β-glucanase may be added as a supplement. Finally, a mash rest temperature of 65–71 °C (149–160 °F) is used to convert the starches in the malt to sugar, which is then usable by the yeast later in the brewing process. Doing the latter rest at the lower end of the range favours β-amylase enzymes, producing more low-order sugars like maltotriose, maltose, and glucose which are more fermentable by the yeast. This in turn creates a beer lower in body and higher in alcohol. A rest closer to the higher end of the range favours α-amylase enzymes, creating more higher-order sugars and dextrins which are less fermentable by the yeast, so a fuller-bodied beer with less alcohol is the result. Duration and pH variances also affect the sugar composition of the resulting wort.[77]
Lautering
[edit]
Lautering is the separation of the wort (the liquid containing the sugar extracted during mashing) from the grains.[78] This is done either in a mash tun outfitted with a false bottom, in a lauter tun, or in a mash filter. Most separation processes have two stages: first wort run-off, during which the extract is separated in an undiluted state from the spent grains, and sparging, in which extract which remains with the grains is rinsed off with hot water. The lauter tun is a tank with holes in the bottom small enough to hold back the large bits of grist and hulls (the ground or milled cereal).[79] The bed of grist that settles on it is the actual filter. Some lauter tuns have provision for rotating rakes or knives to cut into the bed of grist to maintain good flow. The knives can be turned so they push the grain, a feature used to drive the spent grain out of the vessel.[80] The mash filter is a plate-and-frame filter. The empty frames contain the mash, including the spent grains, and have a capacity of around one hectoliter. The plates contain a support structure for the filter cloth. The plates, frames, and filter cloths are arranged in a carrier frame like so: frame, cloth, plate, cloth, with plates at each end of the structure. Newer mash filters have bladders that can press the liquid out of the grains between spargings. The grain does not act like a filtration medium in a mash filter.[81]
Boiling
[edit]After mashing, the beer wort is boiled with hops (and other flavourings if used) in a large tank known as a "copper" or brew kettle – though historically the mash vessel was used and is still in some small breweries.[82] The boiling process is where chemical reactions take place,[64] including sterilization of the wort to remove unwanted bacteria, releasing of hop flavours, bitterness and aroma compounds through isomerization, stopping of enzymatic processes, precipitation of proteins, and concentration of the wort.[83][84] Finally, the vapours produced during the boil volatilise off-flavours, including dimethyl sulfide precursors.[84] The boil is conducted so that it is even and intense – a continuous "rolling boil".[84] The boil on average lasts between 45 and 90 minutes, depending on its intensity, the hop addition schedule, and volume of water the brewer expects to evaporate.[85] At the end of the boil, solid particles in the hopped wort are separated out, usually in a vessel called a "whirlpool".[65]
Brew kettle or copper
[edit]
Copper is the traditional material for the boiling vessel for two main reasons: firstly because copper transfers heat quickly and evenly; secondly because the bubbles produced during boiling, which could act as an insulator against the heat, do not cling to the surface of copper, so the wort is heated in a consistent manner.[86] The simplest boil kettles are direct-fired, with a burner underneath. These can produce a vigorous and favourable boil, but are also apt to scorch the wort where the flame touches the kettle, causing caramelisation and making cleanup difficult. Most breweries use a steam-fired kettle, which uses steam jackets in the kettle to boil the wort.[84] Breweries usually have a boiling unit either inside or outside of the kettle, usually a tall, thin cylinder with vertical tubes, called a calandria, through which wort is pumped.[87]
Whirlpool
[edit]At the end of the boil, solid particles in the hopped wort are separated out, usually in a vessel called a "whirlpool" or "settling tank".[65][88] The whirlpool was devised by Henry Ranulph Hudston while working for the Molson Brewery in 1960 to utilise the so-called tea leaf paradox to force the denser solids known as "trub" (coagulated proteins, vegetable matter from hops) into a cone in the centre of the whirlpool tank.[89][90][91] Whirlpool systems vary: smaller breweries tend to use the brew kettle, larger breweries use a separate tank,[88] and design will differ, with tank floors either flat, sloped, conical or with a cup in the centre.[92] The principle in all is that by swirling the wort the centripetal force will push the trub into a cone at the centre of the bottom of the tank, where it can be easily removed.[88]
Hopback
[edit]A hopback is a traditional additional chamber that acts as a sieve or filter by using whole hops to clear debris (or "trub") from the unfermented (or "green") wort,[93] as the whirlpool does, and also to increase hop aroma in the finished beer.[94][95] It is a chamber between the brewing kettle and wort chiller. Hops are added to the chamber, the hot wort from the kettle is run through it, and then immediately cooled in the wort chiller before entering the fermentation chamber. Hopbacks utilizing a sealed chamber facilitate maximum retention of volatile hop aroma compounds that would normally be driven off when the hops contact the hot wort.[96] While a hopback has a similar filtering effect as a whirlpool, it operates differently: a whirlpool uses centrifugal forces, a hopback uses a layer of whole hops to act as a filter bed. Furthermore, while a whirlpool is useful only for the removal of pelleted hops (as flowers do not tend to separate as easily), in general hopbacks are used only for the removal of whole flower hops (as the particles left by pellets tend to make it through the hopback).[97] The hopback has mainly been substituted in modern breweries by the whirlpool.[98]
Wort cooling
[edit]After the whirlpool, the wort must be brought down to fermentation temperatures 20–26 °C (68–79 °F)[74] before yeast is added. In modern breweries this is achieved through a plate heat exchanger.[99] A plate heat exchanger has sereral ridged plates, which form two separate paths. The wort is pumped into the heat exchanger, and goes through every other gap between the plates.[99] The cooling medium, usually water from a cold liquor tank, goes through the other gaps. The ridges in the plates ensure turbulent flow.[100] A good heat exchanger can drop 95 °C (203 °F) wort to 20 °C (68 °F) while warming the cooling medium from about 10 °C (50 °F) to 80 °C (176 °F). The last few plates often use a cooling medium which can be cooled to below the freezing point, which allows a finer control over the wort-out temperature, and also enables cooling to around 10 °C (50 °F). After cooling, oxygen is often dissolved into the wort to revitalize the yeast and aid its reproduction.[101]
While boiling, it is useful to recover some of the energy used to boil the wort. On its way out of the brewery, the steam created during the boil is passed over a coil through which unheated water flows. By adjusting the rate of flow, the output temperature of the water can be controlled. This is also often done using a plate heat exchanger. The water is then stored for later use in the next mash, in equipment cleaning, or wherever necessary.[102] Another common method of energy recovery takes place during the wort cooling. When cold water is used to cool the wort in a heat exchanger, the water is significantly warmed. In an efficient brewery, cold water is passed through the heat exchanger at a rate set to maximize the water's temperature upon exiting. This now-hot water is then stored in a hot water tank.[102]
Fermenting
[edit]
Fermentation takes place in fermentation vessels which come in various forms, from enormous cylindroconical vessels, through open stone vessels, to wooden vats.[103][104][105] After the wort is cooled and aerated – usually with sterile air – yeast is added to it, and it begins to ferment. It is during this stage that sugars won from the malt are converted into alcohol and carbon dioxide, and the product can be called beer for the first time.
Most breweries today use cylindroconical vessels, or CCVs, which have a conical bottom and a cylindrical top. The cone's angle is typically around 60°, an angle that will allow the yeast to flow towards the cone's apex, but is not so steep as to take up too much vertical space. CCVs can handle both fermenting and conditioning in the same tank. At the end of fermentation, the yeast and other solids which have fallen to the cone's apex can be simply flushed out of a port at the apex. Open fermentation vessels are also used, often for show in brewpubs, and in Europe in wheat beer fermentation. These vessels have no tops, which makes harvesting top-fermenting yeasts very easy. The open tops of the vessels make the risk of infection greater, but with proper cleaning procedures and careful protocol about who enters fermentation chambers, the risk can be well controlled. Fermentation tanks are typically made of stainless steel. If they are simple cylindrical tanks with beveled ends, they are arranged vertically, as opposed to conditioning tanks which are usually laid out horizontally. Only a very few breweries still use wooden vats for fermentation as wood is difficult to keep clean and infection-free and must be repitched more or less yearly.[103][104][105]
Fermentation methods
[edit]
There are three main fermentation methods, warm, cool, and wild or spontaneous. Fermentation may take place in open or closed vessels. There may be a secondary fermentation which can take place in the brewery, in the cask or in the bottle.[106]
Brewing yeasts are traditionally classed as "top-cropping" (or "top-fermenting") and "bottom-cropping" (or "bottom-fermenting"); the yeasts classed as top-fermenting are generally used in warm fermentations, where they ferment quickly, and the yeasts classed as bottom-fermenting are used in cooler fermentations where they ferment more slowly.[107] Yeast were termed top or bottom cropping, because the yeast was collected from the top or bottom of the fermenting wort to be reused for the next brew.[108] This terminology is somewhat inappropriate in the modern era; after the widespread application of brewing mycology it was discovered that the two separate collecting methods involved two different yeast species that favoured different temperature regimes, namely Saccharomyces cerevisiae in top-cropping at warmer temperatures and Saccharomyces pastorianus in bottom-cropping at cooler temperatures.[109] As brewing methods changed in the 20th century, cylindro-conical fermenting vessels became the norm and the collection of yeast for both Saccharomyces species is done from the bottom of the fermenter. Thus the method of collection no longer implies a species association. There are a few remaining breweries who collect yeast in the top-cropping method, such as Samuel Smiths brewery in Yorkshire, Marstons in Staffordshire and several German hefeweizen producers.[108]
For both types, yeast is fully distributed through the beer while it is fermenting, and both equally flocculate (clump together and precipitate to the bottom of the vessel) when fermentation is finished. By no means do all top-cropping yeasts demonstrate this behaviour, but it features strongly in many English yeasts that may also exhibit chain forming (the failure of budded cells to break from the mother cell), which is in the technical sense different from true flocculation. The most common top-cropping brewer's yeast, Saccharomyces cerevisiae, is the same species as the common baking yeast. However, baking and brewing yeasts typically belong to different strains, cultivated to favour different characteristics: baking yeast strains are more aggressive, in order to carbonate dough in the shortest amount of time; brewing yeast strains act slower, but tend to tolerate higher alcohol concentrations (normally 12–15% abv is the maximum, though under special treatment some ethanol-tolerant strains can be coaxed up to around 20%).[110] Modern quantitative genomics has revealed the complexity of Saccharomyces species to the extent that yeasts involved in beer and wine production commonly involve hybrids of so-called pure species. As such, the yeasts involved in what has been typically called top-cropping or top-fermenting ale may be both Saccharomyces cerevisiae and complex hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii. Three notable ales, Chimay, Orval and Westmalle, are fermented with these hybrid strains, which are identical to wine yeasts from Switzerland.[111]
Warm fermentation
[edit]In general, yeasts such as Saccharomyces cerevisiae are fermented at warm temperatures between 15 and 20 °C (59 and 68 °F), occasionally as high as 24 °C (75 °F),[112] while the yeast used by Brasserie Dupont for saison ferments even higher at 29 to 35 °C (84 to 95 °F).[113] They generally form a foam on the surface of the fermenting beer, which is called barm, as during the fermentation process its hydrophobic surface causes the flocs to adhere to CO2 and rise; because of this, they are often referred to as "top-cropping" or "top-fermenting"[114] – though this distinction is less clear in modern brewing with the use of cylindro-conical tanks.[115] Generally, warm-fermented beers, which are usually termed ale, are ready to drink within three weeks after the beginning of fermentation, although some brewers will condition or mature them for several months.[116]
Cool fermentation
[edit]
When a beer has been brewed using a cool fermentation of around 10 °C (50 °F), compared to typical warm fermentation temperatures of 18 °C (64 °F),[117][118] then stored (or lagered) for typically several weeks (or months) at temperatures close to freezing point, it is termed a "lager".[119] During the lagering or storage phase several flavour components developed during fermentation dissipate, resulting in a "cleaner" flavour.[120][121] Though it is the slow, cool fermentation and cold conditioning (or lagering) that defines the character of lager,[122] the main technical difference is with the yeast generally used, which is Saccharomyces pastorianus.[123] Technical differences include the ability of lager yeast to metabolize melibiose,[124] and the tendency to settle at the bottom of the fermenter (though ale yeasts can also become bottom settling by selection);[124] though these technical differences are not considered by scientists to be influential in the character or flavour of the finished beer, brewers feel otherwise – sometimes cultivating their own yeast strains which may suit their brewing equipment or for a particular purpose, such as brewing beers with a high abv.[125][126][127][128]

Brewers in Bavaria had for centuries been selecting cold-fermenting yeasts by storing ("lagern") their beers in cold alpine caves. The process of natural selection meant that the wild yeasts that were most cold tolerant would be the ones that would remain actively fermenting in the beer that was stored in the caves. A sample of these Bavarian yeasts was sent from the Spaten brewery in Munich to the Carlsberg brewery in Copenhagen in 1845 who began brewing with it. In 1883 Emile Hansen completed a study on pure yeast culture isolation and the pure strain obtained from Spaten went into industrial production in 1884 as Carlsberg yeast No 1. Another specialized pure yeast production plant was installed at the Heineken Brewery in Rotterdam the following year and together they began the supply of pure cultured yeast to brewers across Europe.[129][130] This yeast strain was originally classified as Saccharomyces carlsbergensis, a now defunct species name which has been superseded by the currently accepted taxonomic classification Saccharomyces pastorianus.[131]
Spontaneous fermentation
[edit]Lambic beers are historically brewed in Brussels and the nearby Pajottenland region of Belgium without any yeast inoculation.[132][133] The wort is cooled in open vats (called "coolships"), where the yeasts and microbiota present in the brewery (such as Brettanomyces)[134] are allowed to settle to create a spontaneous fermentation,[135] and are then conditioned or matured in oak barrels for typically one to three years.[136]
Conditioning
[edit]After an initial or primary fermentation, beer is conditioned, matured or aged,[137] in one of several ways,[138] which can take from 2 to 4 weeks, several months, or several years, depending on the brewer's intention for the beer. The beer is usually transferred into a second container, so that it is no longer exposed to the dead yeast and other debris (also known as "trub") that have settled to the bottom of the primary fermenter. This prevents the formation of unwanted flavours and harmful compounds such as acetaldehyde.[139]
- Kräusening
Kräusening (pronounced KROY-zen-ing[140]) is a conditioning method in which fermenting wort is added to the finished beer.[141] The active yeast will restart fermentation in the finished beer, and so introduce fresh carbon dioxide; the conditioning tank will be then sealed so that the carbon dioxide is dissolved into the beer producing a lively "condition" or level of carbonation.[141] The kräusening method may also be used to condition bottled beer.[141]
- Lagering
Lagers are stored at cellar temperature or below for 1–6 months while still on the yeast.[142] The process of storing, or conditioning, or maturing, or aging a beer at a low temperature for a long period is called "lagering", and while it is associated with lagers, the process may also be done with ales, with the same result – that of cleaning up various chemicals, acids and compounds.[143]
- Secondary fermentation
During secondary fermentation, most of the remaining yeast will settle to the bottom of the second fermenter, yielding a less hazy product.[144]
- Bottle fermentation
Some beers undergo an additional fermentation in the bottle giving natural carbonation.[145] This may be a second and/or third fermentation. They are bottled with a viable yeast population in suspension. If there is no residual fermentable sugar left, sugar or wort or both may be added in a process known as priming. The resulting fermentation generates CO2 that is trapped in the bottle, remaining in solution and providing natural carbonation. Bottle-conditioned beers may be either filled unfiltered direct from the fermentation or conditioning tank, or filtered and then reseeded with yeast.[146]
- Cask conditioning

Cask ale (or cask-conditioned beer) is unfiltered, unpasteurised beer that is conditioned by a secondary fermentation in a metal, plastic or wooden cask. It is dispensed from the cask by being either poured from a tap by gravity, or pumped up from a cellar via a beer engine (hand pump).[147] Sometimes a cask breather is used to keep the beer fresh by allowing carbon dioxide to replace oxygen as the beer is drawn off the cask.[148] Until 2018, the Campaign for Real Ale (CAMRA) defined real ale as beer "served without the use of extraneous carbon dioxide", which would disallow the use of a cask breather,[149] a policy which was reversed in April 2018 to allow beer served with the use of cask breathers to meet its definition of real ale.[150]
- Barrel-ageing
Barrel-ageing (US: Barrel aging) is the process of ageing beer in wooden barrels to achieve a variety of effects in the final product. Sour beers such as lambics are fully fermented in wood, while other beers are aged in barrels which were previously used for maturing wines or spirits. In 2016 "Craft Beer and Brewing" wrote: "Barrel-aged beers are so trendy that nearly every taphouse and beer store has a section of them.[151]
Filtering
[edit]
Filtering stabilises the flavour of beer, holding it at a point acceptable to the brewer, and preventing further development from the yeast, which under poor conditions can release negative components and flavours.[152] Filtering also removes haze, clearing the beer, and so giving it a "polished shine and brilliance".[153] Beer with a clear appearance has been commercially desirable for brewers since the development of glass vessels for storing and drinking beer, along with the commercial success of pale lager, which – due to the lagering process in which haze and particles settle to the bottom of the tank and so the beer "drops bright" (clears) – has a natural bright appearance and shine.[154]
There are several forms of filters; they may be in the form of sheets or "candles", or they may be a fine powder such as diatomaceous earth (also called kieselguhr),[155] which is added to the beer to form a filtration bed which allows liquid to pass, but holds onto suspended particles such as yeast.[156] Filters range from rough filters that remove much of the yeast and any solids (e.g., hops, grain particles) left in the beer,[157] to filters tight enough to strain colour and body from the beer.[citation needed] Filtration ratings are divided into rough, fine, and sterile.[citation needed] Rough filtration leaves some cloudiness in the beer, but it is noticeably clearer than unfiltered beer.[citation needed] Fine filtration removes almost all cloudiness.[citation needed] Sterile filtration removes almost all microorganisms.[citation needed] Polysaccharides, proteins, yeast, bacteria and the brewing process may affect filtration.[158].
- Sheet (pad) filters
These filters use sheets that allow only particles smaller than a given size to pass through. The sheets are placed into a filtering frame, sanitized (with boiling water, for example) and then used to filter the beer. The sheets can be flushed if the filter becomes blocked. The sheets are usually disposable and are replaced between filtration sessions. Often the sheets contain powdered filtration media to aid in filtration.
Pre-made filters have two sides. One with loose holes, and the other with tight holes. Flow goes from the side with loose holes to the side with the tight holes, with the intent that large particles get stuck in the large holes while leaving enough room around the particles and filter medium for smaller particles to go through and get stuck in tighter holes.
Sheets are sold in nominal ratings, and typically 90% of particles larger than the nominal rating are caught by the sheet.
- Kieselguhr filters
Filters that use a powder medium are considerably more complicated to operate, but can filter much more beer before regeneration. Common media include diatomaceous earth and perlite.
By-products
[edit]

Brewing by-products are "spent grain" and the sediment (or "dregs") from the filtration process which may be dried and resold as "brewers dried yeast" for poultry feed,[159] or made into yeast extract which is used in brands such as Vegemite and Marmite.[160] The process of turning the yeast sediment into edible yeast extract was discovered by German scientist Justus von Liebig.[161]
Brewer's spent grain (also called spent grain, brewer's grain or draff) is the main by-product of the brewing process;[162] it consists of the residue of malt and grain which remains in the lauter tun after the lautering process.[163] It consists primarily of grain husks, pericarp, and fragments of endosperm.[164] As it mainly consists of carbohydrates and proteins,[164] and is readily consumed by animals,[165] spent grain is used in animal feed.[165] Spent grains can also be used as fertilizer, whole grains in bread,[166] as well as in the production of flour and biogas.[167][168] Spent grain is also an ideal medium for growing mushrooms, such as shiitake, and some breweries are already either growing their own mushrooms or supplying spent grain to mushroom farms.[169] Spent grains can be used in the production of red bricks, to improve the open porosity and reduce thermal conductivity of the ceramic mass.[170]
Brewing industry
[edit]The brewing industry is a global business, consisting of several dominant multinational companies and many thousands of other producers known as microbreweries or regional breweries or craft breweries depending on size, region, and marketing preference.[22][171] More than 133 billion liters (3.5×1010 U.S. gallons; 2.9×1010 imperial gallons) are sold per year—producing total global revenues of $294.5 billion (£147.7 billion) as of 2006.[172] SABMiller became the largest brewing company in the world when it acquired Royal Grolsch, brewer of Dutch premium beer brand Grolsch.[173] InBev was the second-largest beer-producing company in the world and Anheuser-Busch held the third spot, but after the acquisition of Anheuser-Busch by InBev, the new Anheuser-Busch InBev company is currently the largest brewer in the world.[174]
Brewing at home is subject to regulation and prohibition in many countries. Restrictions on homebrewing were lifted in the UK in 1963,[175] Australia followed suit in 1972,[176] and the US in 1978, though individual states were allowed to pass their own laws limiting production.[177]
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External links
[edit]Brewing
View on GrokipediaBrewing is the process of producing beer from malted cereals and grains, primarily barley, by steeping them in hot water to extract fermentable sugars, boiling the resulting wort with hops for bitterness, aroma, and preservation, and fermenting with yeast to generate alcohol and carbon dioxide.[1][2] The core biochemical transformations rely on enzymes activated during malting to hydrolyze starches into maltose and other sugars, which yeast then metabolizes under anaerobic conditions, yielding ethanol concentrations typically between 4% and 6% by volume in standard beers.[2] Originating around 4000 BCE in Mesopotamia, where Sumerians documented recipes on clay tablets, brewing predates written history in some regions and facilitated early urbanization by providing a nutritious, pathogen-resistant beverage superior to untreated water in pre-modern sanitation conditions.[3][4] Key steps include milling the malt, mashing at controlled temperatures (around 60-70°C) to optimize enzyme activity, lautering to separate solids, boiling for 60-90 minutes to isomerize hop acids and sterilize, cooling, primary fermentation (lasting days to weeks), and secondary conditioning for flavor maturation.[2][5] Hops, introduced in medieval Europe, revolutionized brewing by inhibiting spoilage bacteria and balancing malt sweetness with iso-alpha acids, enabling longer storage and diverse styles from pale lagers to robust stouts.[6] While industrial scaling post-Industrial Revolution standardized production for efficiency, controversies arose over adulteration with non-traditional ingredients and the health impacts of excessive consumption, though moderate intake correlates with cardiovascular benefits in empirical studies, underscoring brewing's dual role as cultural staple and biochemical engineering feat.[6][7]
History
Prehistoric and Ancient Origins
The earliest archaeological evidence for brewing a fermented cereal beverage dates to approximately 11,000 BCE, identified through chemical residues of tartaric acid, syringic acid, and malvidin in stone mortars from Raqefet Cave in Israel, associated with the Natufian culture of semi-nomadic foragers.[8][9] These residues suggest a beer-like gruel produced from wild cereals such as wheat and barley, potentially used in burial rituals as indicated by the context of human interments and feasting remains at the site.[10] Independent evidence from southern China, around 7000 BCE, includes chaff-impressed pottery and rice husks from a platform mound site, analyzed via starch grain and phytolith analysis, pointing to beer consumption in funerary practices involving millet, barley, and Job's tears.[11] In Mesopotamia, brewing transitioned to systematic production by the Sumerians around 4000 BCE, as evidenced by cuneiform texts documenting beer as a staple ration, trade good, and ritual offering, with at least nine varieties described in Uruk-period records including golden, dark, and sweet types.[12] The process involved fermenting barley-based bappir bread in water, flavored with herbs or dates, and regulated by laws such as those in the Code of Hammurabi (circa 1750 BCE), which prescribed penalties for substandard beer.[13] The Hymn to Ninkasi, inscribed around 1800 BCE, provides the oldest known brewing recipe, detailing the mashing of barley, straining, and fermentation steps under the patronage of the goddess of beer.[12] Ancient Egyptian brewing paralleled Sumerian developments, with predynastic evidence from Hierakonpolis (circa 4000–3500 BCE) revealing specialized beer jars and production facilities using emmer wheat and barley, confirmed by residue analysis showing fermentation markers.[14] By the Old Kingdom (circa 3000 BCE), large-scale industrial brewing occurred, as demonstrated by a Theban necropolis site with vats capable of yielding up to 22,400 liters of beer per batch, likely for workers' rations and elite consumption.[15] Egyptian beer, often thick and porridge-like due to incomplete straining, served as daily sustenance, payment for labor—such as pyramid builders receiving four liters daily—and offerings in tombs, with textual and artistic depictions illustrating women-dominated household production evolving into state-supervised operations.[16][17]Medieval Development and Guild Systems
In the early Middle Ages, brewing developed significantly within monastic communities across Europe, where monks refined production techniques to create a safe, nutritious beverage amid contaminated water sources, often using barley and herbs for fermentation. The Carolingian Empire from the mid-8th century promoted beer as a rehabilitated staple following Christianization, with monasteries establishing breweries for self-sufficiency and trade. The Synod of Aachen in 816 explicitly permitted monastic brewing within abbey walls, enabling systematic production that spread to regions including Germany, the British Isles, and Scandinavia by the 9th-10th centuries.[18][19][20] Urban brewing emerged alongside this monastic tradition, initially dominated by women termed alewives in England and parts of northern Europe, who produced unhopped ale in domestic settings for local consumption and sale, yielding a reliable economic role through the 13th-14th centuries. Professionalization accelerated with the 14th-century introduction of hopped beer by immigrants, such as Dutch brewers in England, which extended shelf life and supported larger-scale operations, gradually supplanting ale and shifting production toward commercial enterprises. By the late 14th century, male brewers increasingly controlled the trade, with regulations and taxation favoring hopped beer over traditional ale methods.[21][22][23] Craft guilds formed from the 12th century in European cities, including brewers among early associations to enforce quality standards, mediate disputes, and safeguard monopolies, with documented evidence of a London brewers' guild appearing in 1292 city records. In Germany, brewers ranked among the inaugural trade guilds, fostering urban breweries operational by 1300 in northern regions and laying groundwork for later regulations. These guilds marginalized independent alewives through entry barriers and oversight, centralizing brewing under male-dominated structures that prioritized consistency and economic protection until the Renaissance.[24][25][26][27]Industrial Revolution and Scientific Advances
The Industrial Revolution, beginning in the late 18th century, transformed brewing from small-scale artisanal practices to mechanized industrial production, primarily in Britain and later Europe. Innovations such as the thermometer, introduced around 1760, enabled precise temperature control during mashing, preventing inconsistent results from reliance on subjective judgment.[28] [29] The hydrometer, developed in the 1770s, allowed measurement of wort density to assess potential alcohol content and fermentation progress, facilitating recipe standardization and quality control.[30] [28] These instruments, combined with the drum roaster for producing crystal malts in the 1810s, supported the creation of diverse beer styles like porters and pale ales on a larger scale.[29] Steam power further accelerated industrialization; James Watt's improvements to the steam engine in 1765 powered pumps, mills, and kettles, reducing labor and enabling continuous operations.[30] By 1801, 14 steam engines were in use across London breweries, allowing enterprises like Whitbread to expand output dramatically from thousands to tens of thousands of barrels annually.[31] Centers like Burton-upon-Trent emerged as hubs for pale ale production, leveraging local gypsum-rich water for efficient hops extraction and scaling via rail transport of barley and coal.[31] This mechanization lowered costs, increased consistency, and met rising urban demand from industrialized workforces, though it initially favored top-fermenting ales over lagers due to ambient temperature limitations.[32] Scientific breakthroughs in the mid-to-late 19th century provided causal explanations for fermentation, shifting brewing toward microbiology. Louis Pasteur's 1857 experiments revealed yeast as the agent of alcoholic fermentation, refuting spontaneous generation and identifying lactic acid bacteria as causes of beer souring.[6] His 1860s development of pasteurization—heating beer to 60–70°C—sterilized contaminants without altering flavor, extending shelf life and enabling export.[33] [34] Building on this, Emil Christian Hansen at Carlsberg's laboratory isolated pure yeast strains in 1883 using micromanipulation, producing the first single-cell culture for lager brewing and named Saccharomyces carlsbergensis.[35] [36] These advances minimized wild yeast contamination, ensured reproducible attenuation, and laid foundations for controlled microbiology, though adoption varied due to initial costs and resistance from traditional brewers.[37]20th Century Expansion and Modern Innovations
The brewing industry underwent significant expansion in the early 20th century through industrialization and technological standardization, enabling large-scale production of consistent lager beers for global markets. In the United States, beer production surged from 3.6 million barrels in 1865 to over 66 million barrels by 1914, driven by immigration, urbanization, and refrigeration allowing year-round brewing.[32] However, Prohibition from 1920 to 1933 halted alcoholic beer production, forcing major brewers like Anheuser-Busch and Pabst to pivot to near-beer, soft drinks, and malt extracts, with only a few surviving through diversification.[38] Post-repeal in 1933 and after World War II, consolidation accelerated, with breweries adopting stainless steel equipment, pasteurization for shelf stability, and canned packaging introduced in the 1930s, reducing costs and expanding distribution via national brands.[32] [39] Worldwide, the 20th century saw the rise of the industrial brewery model, where engineers optimized processes for efficiency, including automated fermentation controls and filtration systems to produce uniform, exportable beers amid wartime rationing and post-war economic booms.[40] By mid-century, multinational corporations dominated, with advancements like stainless steel kegs in the 1950s enabling hygienic, scalable operations.[39] This era's focus on mass production, however, led to flavor homogenization, prompting consumer backlash. The late 20th century marked the craft beer revolution, countering industrial uniformity with small-scale, innovative brewing. In the US, Fritz Maytag's 1965 acquisition and revival of Anchor Brewing introduced steam beer and emphasized quality ingredients, laying groundwork for microbreweries.[41] Homebrewing legalization in 1978 under President Jimmy Carter spurred experimentation, followed by New Albion Brewery's opening in 1976 as the first post-Prohibition microbrewery.[42] [43] By 1994, US craft breweries numbered nearly 500, fueled by accessible equipment and demand for diverse styles.[44] In the UK, the Campaign for Real Ale (CAMRA) formed in 1971 to advocate traditional cask ales against pasteurization.[45] Modern innovations since the 1980s integrate precision technology with artisanal methods, enhancing efficiency and sustainability. Unitank fermenters allow pressure fermentation for lagers and ales in one vessel, while advanced filtration and IoT sensors enable real-time monitoring of variables like temperature and pH for consistent quality.[46] [47] Automation and AI optimize processes, reducing waste, and biotechnology explores yeast strains for novel flavors, though traditional open fermentation persists in craft settings.[48] Eco-friendly practices, including energy-efficient equipment and water recycling, address resource demands, with modular systems supporting small-batch innovation like dry-hopping and adjunct experimentation.[49] These developments have expanded brewing's accessibility, from home setups to global craft exports, balancing scale with diversity.[50]Ingredients
Water Quality and Treatment
Water constitutes 90 to 95 percent of beer by volume, making its quality foundational to enzymatic reactions, yeast health, flavor development, and overall beer character.[51] Impurities such as chlorine, chloramine, organic matter, and excessive minerals must be managed, as they can inhibit fermentation, promote off-flavors like medicinal chlorophenols, or disrupt pH balance critical for mashing.[52] [53] The mineral profile—primarily calcium (Ca²⁺), magnesium (Mg²⁺), sodium (Na⁺), sulfate (SO₄²⁻), chloride (Cl⁻), and bicarbonate (HCO₃⁻)—directly impacts brewing outcomes. Calcium at 50 to 150 ppm stabilizes mash enzymes like alpha- and beta-amylase, aids protein coagulation during boiling, and enhances yeast flocculation for clearer beer.[54] [55] Magnesium supports similar functions but at lower optimal levels (10 to 30 ppm), as excesses above 50 ppm impart bitterness or yeast stress.[54] Sulfate sharpens hop bitterness and dryness, with levels up to 300 ppm suitable for pale ales but risking astringency beyond that.[54] Chloride promotes malt sweetness and fuller body, ideally balanced against sulfate in ratios of 0.5 to 1.5 for most styles.[54] High bicarbonate raises mash pH, buffering acidity from pale malts and potentially yielding thin, chalky beers unless acidified.[54] Regional water profiles have historically shaped beer styles through natural geology. In Burton-upon-Trent, England, groundwater rich in gypsum yields sulfate levels of 600 to 1,200 ppm and calcium around 300 ppm, enabling the high-hopped, attenuated pale ales and India pale ales that emerged in the 18th and 19th centuries by countering haze in boiled, heavily hopped worts.[56] Conversely, Pilsen's soft water, with total minerals under 100 ppm, supports the crisp, low-bitterness profile of pale lagers developed there in 1842.[57] Dublin's moderately hard water, higher in chloride and lower in sulfate, favors the malty sweetness of stouts like those from Guinness since 1759.[58] Contemporary treatment begins with source analysis via ion chromatography to quantify minerals and contaminants. Chlorine and chloramine are removed via activated carbon filtration or ascorbic acid to prevent yeast inhibition and medicinal notes.[59] [52] Reverse osmosis (RO), employing semi-permeable membranes under high pressure, strips 95 to 99 percent of dissolved solids, yielding near-pure water as a neutral base for rebuilding style-specific profiles with salts like calcium sulfate (gypsum) or calcium chloride.[60] [61] pH adjustment targets 5.2 to 5.6 for mashing to optimize starch conversion and avoid tannin extraction, often via lactic acid or phosphoric acid additions.[51] Softening via ion exchange reduces excess hardness where RO is impractical, while UV sterilization or ozone addresses microbial risks in non-municipal sources.[59] These methods allow brewers to replicate historical profiles or innovate, unconstrained by local geology.[62]Malted Grains and Adjuncts
, form the bulk of the grain bill at 70-100%, supplying neutral fermentables.[67] Specialty malts, including crystal malts stewed to caramelize sugars for sweetness and body, and roasted malts like black malt heated to 200-250°C for dark colors and coffee-like flavors, are used in smaller proportions (5-20%) to impart specific characteristics.[67] Other malted grains, such as wheat for haze and head retention in hefeweizens or rye for spicy notes, supplement barley in specialty styles.[68] Adjuncts are unmalted sources of carbohydrates added to the mash or boil to supplement malted grains, providing additional fermentable sugars without contributing significant enzymes or flavors.[69] Common cereal adjuncts include flaked corn (maize) and rice, which require gelatinization at high temperatures (70-80°C) to make starches accessible, often comprising 20-40% of the grain bill in American lagers to achieve a lighter body and higher alcohol yield at lower cost.[69] Sugar adjuncts like corn syrup, cane sugar, or honey, added during boiling, ferment completely to increase attenuation and dryness, as seen in Belgian strong ales where they can constitute up to 30% of extract.[70] While adjuncts dilute malt-derived flavors and reduce foam stability due to lower protein content, they enable efficient scaling in large-scale production, with historical use dating to the 19th century for economic reasons in adjunct-heavy styles.[71] Brewers must balance adjunct levels to avoid thin mouthfeel or excessive haze from incomplete conversion.[71]Hops and Flavoring Agents
Hops, the dried female flower cones of the Humulus lupulus plant, serve as the primary flavoring agent in most modern beers, imparting bitterness, aroma, and subtle flavors while contributing to preservation.[72] The bitterness arises from alpha acids, primarily humulone, cohumulone, and adhumulone, which isomerize during wort boiling into iso-alpha acids that provide the characteristic sharp taste balancing malt sweetness.[73] Essential oils, including terpenes like myrcene, humulene, and farnesene, deliver aromatic compounds such as citrus, pine, or floral notes, with over 3,000 flavor-active volatiles identified in hops.[74] Additionally, hops exhibit antimicrobial properties due to polyphenols and bitter acids, extending beer shelf life by inhibiting spoilage bacteria and wild yeasts.[75] The use of hops in brewing originated in Europe around 822 AD, with the earliest documented reference in a French abbey record, though widespread adoption occurred in Germany and the Low Countries by the 12th-15th centuries, replacing earlier herbal mixtures for standardization and preservation efficacy.[76] In the brewing process, hops are added at different stages: early boil for bittering (high-alpha varieties to maximize isomerization), mid-boil for flavor, and late or post-boil (including dry-hopping) for aroma preservation, as heat degrades volatile oils.[77] Alpha acid content varies by variety, typically 2-18%, influencing hop quantity; for instance, bittering hops like Magnum (12-14% alpha) require less mass than aroma types.[78] Hop varieties are classified by origin, alpha acid levels, and sensory profiles. European noble hops, such as Hallertau (3-5.5% alpha), Saaz (3-5%), Tettnang (4-5%), and Spalt (4-5%), offer refined, earthy, and herbal aromas with low cohumulone for smoother bitterness, traditionally used in lagers.[79] American varieties, bred for higher alpha acids and bold flavors, include Cascade (5-9% alpha, citrus/pine), Centennial (9-11%, floral/lemon), and Citra (11-15%, tropical fruit), dominating craft IPAs for their potent oil content.[80] Dual-purpose hops like Chinook (12-14% alpha) balance bitterness and aroma with spicy, grapefruit notes.[81]| Variety Type | Examples | Alpha Acid Range (%) | Key Aromas |
|---|---|---|---|
| Noble (European) | Hallertau, Saaz | 3-5.5 | Herbal, floral, earthy |
| Aroma (American) | Cascade, Amarillo | 5-10 | Citrus, tropical |
| Bittering | Magnum, Warrior | 12-18 | Neutral, high yield |
Yeast Strains and Microbiology
Yeast plays a central role in brewing by fermenting wort sugars into ethanol, carbon dioxide, and various flavor compounds such as esters, phenols, and higher alcohols, which define beer styles.[85] The primary domesticated species used are Saccharomyces cerevisiae for top-fermenting ales and Saccharomyces pastorianus for bottom-fermenting lagers.[86] [2] S. cerevisiae strains typically ferment at warmer temperatures (15–24°C), producing fruitier profiles due to greater ester formation, while S. pastorianus, a natural hybrid of S. cerevisiae and Saccharomyces eubayanus, ferments at cooler temperatures (7–13°C) and yields cleaner, malt-forward beers with higher attenuation.[87] [88] Yeast strains exhibit genetic diversity even within species, influencing fermentation efficiency, flocculation, and sensory outcomes; for instance, commercial lager strains vary in their ability to metabolize maltotriose, a key sugar comprising up to 30% of wort carbohydrates.[86] Flocculation, the reversible aggregation of yeast cells via cell-wall lectins binding mannose residues, occurs toward fermentation's end, promoting sedimentation in lagers for clarity without filtration, though excessive flocculation can lead to incomplete attenuation and residual sweetness.[89] [90] Top-fermenting S. cerevisiae often forms a krausen head due to CO₂ entrapment in pseudohyphae, allowing harvesting from the surface, whereas bottom-fermenters flocculate more uniformly and settle.[91] The shift to pure yeast cultures revolutionized brewing microbiology, eliminating inconsistent wild fermentations plagued by off-flavors. In 1883, Emil Christian Hansen at Carlsberg Brewery isolated the first single-cell pure culture of lager yeast (Saccharomyces carlsbergensis, now reclassified under S. pastorianus), using micromanipulation to propagate contaminant-free strains, which reduced spoilage and standardized production.[92] [93] Brewers now propagate and pitch specific strains at densities of 0.75–1.5 million viable cells per milliliter of wort to ensure rapid colonization and minimize contamination risks.[94] Fermentation microbiology involves anaerobic glycolysis where yeast converts glucose via pyruvate decarboxylation to ethanol (typically 4–6% ABV in standard beers) and CO₂, alongside side reactions producing fusel alcohols and vicinal diketones like diacetyl, which S. pastorianus reduces more effectively at low temperatures.[85] Yeast viability and vitality, assessed via cell counts and staining, are critical, as stressed cells (e.g., from high gravity worts) yield unbalanced esters.[95] Contaminants, including wild yeasts like Brettanomyces (producing earthy phenols) or bacteria such as Lactobacillus and Pediococcus (causing sourness and turbidity), thrive in unhopped or immature beer, necessitating aseptic propagation and sanitation; gram-negative anaerobes like Pectinatus and Megasphaera produce hydrogen sulfide off-notes in packaged products.[96] [97] [98] Specialty strains, such as Brettanomyces for funky lambics or diastaticus variants enabling super-attenuated beers, are intentionally used in niche styles but pose risks of over-attenuation or gushing in standard brews due to starch-degrading enzymes.[99] Modern strain selection draws from genomic analyses revealing hybrid vigor in S. pastorianus for cold tolerance and sugar utilization, enabling consistent quality across scales.[100]Scientific Principles
Biochemical and Chemical Reactions
During malting, the Maillard reaction occurs between reducing sugars and amino acids in germinating barley, producing melanoidins that contribute to beer color, flavor compounds like pyrazines, and antioxidants, with the extent depending on kilning temperature and duration.[101] This non-enzymatic browning reaction, accelerated above 50°C, also generates advanced glycation end-products and dicarbonyls, influencing malt type-specific profiles in pale versus crystal malts.[102] In mashing, α-amylase and β-amylase from malt hydrolyze starch into fermentable sugars: α-amylase endohydrolyzes α-1,4-glucosidic bonds to yield maltose, maltotriose, and dextrins, while β-amylase exohydrolyzes from non-reducing ends to produce maltose, with optimal activities at 65–70°C for α and 55–60°C for β before thermal inactivation.[103] Gelatinization precedes hydrolysis, disrupting starch granule structure at 55–65°C for barley, enabling enzyme access and yielding up to 80% fermentable extract, though limit dextrins remain partially unfermentable.[104] Wort boiling isomerizes hop α-acids (humulones) to iso-α-acids via acyl migration and decarboxylation, requiring 60 minutes at 100°C for approximately 30% conversion and bitterness units of 20–40 IBUs in standard ales, with rates following pseudo-first-order kinetics accelerated by divalent cations like magnesium.[105] Concurrently, volatile thiols and polyphenols polymerize, reducing astringency, while Maillard reactions further develop caramel notes.[106] Fermentation involves yeast-mediated glycolysis converting glucose to pyruvate, followed by alcoholic fermentation yielding ethanol (up to 5–12% ABV) and CO2 via pyruvate decarboxylase and alcohol dehydrogenase, with Saccharomyces cerevisiae strains optimizing under anaerobic conditions at 10–20°C.[85] Secondary metabolism produces esters like isoamyl acetate through alcohol acetyltransferase condensing fusel alcohols and acetyl-CoA, influenced by yeast genetics, temperature, and wort FAN levels, contributing fruity aromas at 1–20 mg/L.[107] Higher alcohols form via Ehrlich pathway from amino acids, while sulfides like H2S arise from sulfate reduction, dissipating during maturation.[108]Microbiological Processes and Controls
The primary microbiological process in brewing is alcoholic fermentation, dominated by yeast species such as Saccharomyces cerevisiae for top-fermenting ales and Saccharomyces pastorianus for bottom-fermenting lagers. During fermentation, yeast metabolizes wort carbohydrates, primarily glucose, through glycolysis to produce pyruvate, which is then decarboxylated to acetaldehyde and reduced to ethanol, yielding a net of two ATP molecules per glucose and releasing carbon dioxide as a byproduct.[91] This process also generates secondary metabolites like esters, higher alcohols, and sulfur compounds that contribute to beer flavor profiles, with ester production occurring via condensation of fusel alcohols with acyl-CoA derivatives within yeast cells.[109] [110] Fermentation proceeds in distinct phases: an initial lag phase for yeast adaptation, followed by rapid growth and attenuation where sugars are consumed, typically reducing specific gravity from around 1.050 to 1.010-1.015 over 3-10 days depending on style and temperature.[111] Microbial activity extends beyond yeast, with potential involvement of lactic acid bacteria (LAB) such as Lactobacillus and Pediococcus in unintentional souring or intentional mixed fermentations, though these are generally undesired in standard lager and ale production due to off-flavors like diacetyl or acidity.[112] [Acetic acid bacteria](/page/Acetic acid bacteria) and strict anaerobes like Pectinatus or Zymomonas can produce spoilage compounds such as hydrogen sulfide or excessive acetic acid if conditions favor their growth.[98] In traditional spontaneous fermentations, diverse microbial consortia including wild yeasts and bacteria contribute to complex flavors via amylolysis and acid production, but modern brewing minimizes such variability.[113] Controls for microbiological stability emphasize prevention of contamination through sanitation, selective conditions favoring yeast, and monitoring. Wort boiling prior to fermentation sterilizes the medium by denaturing proteins and killing vegetative microbes, while hops provide antimicrobial peptides like iso-alpha acids that inhibit Gram-positive bacteria more than yeast.[114] Pitching sufficient viable yeast (typically 0.75-1.5 million cells per milliliter per degree Plato) ensures rapid colonization and pH drop to below 4.5, which suppresses bacterial proliferation, augmented by controlled temperatures (e.g., 10-20°C for ales, 4-12°C for lagers) to optimize yeast activity over slower-growing spoilers.[115] Sanitation protocols involve cleaning agents like caustic soda followed by sanitizers such as peracetic acid, verified via ATP bioluminescence for residual microbes, and equipment design to minimize dead spaces.[116] [117] Detection and mitigation rely on culture-independent methods like PCR for rapid identification of spoilers such as Pediococcus or wild yeasts, alongside traditional plating on selective media (e.g., Wallerstein Labs Differential Agar for bacteria versus yeast).[118] Post-fermentation, filtration, pasteurization at 60-72°C for 15-30 seconds, or sterile filtration prevent recontamination during packaging, with ongoing monitoring essential as biofilms in filling lines account for up to 50% of late-stage contaminations.[119] [120] These measures maintain beer stability, as unchecked microbial growth can lead to over-carbonation, turbidity, or sensory defects detectable within weeks.[97]Brewing Process
Mashing and Enzymatic Conversion
Mashing involves mixing milled malted grains, known as grist, with hot water to form a porridge-like mash, enabling enzymatic hydrolysis of starches into fermentable sugars and dextrins.[121] This step typically occurs in a mash tun, an insulated vessel that maintains precise temperatures essential for enzyme activity.[122] The process activates endogenous enzymes from the malt, primarily alpha-amylase and beta-amylase, which break down gelatinized starches through hydrolysis of glycosidic bonds.[123] Beta-amylase, optimal at 60–65°C (140–149°F), cleaves maltose units from the non-reducing ends of amylose chains, favoring production of fermentable sugars for higher attenuation and drier beers.[124] Alpha-amylase, active at higher temperatures of 68–72°C (154–162°F), randomly hydrolyzes internal alpha-1,4 linkages, yielding dextrins and limit dextrins that contribute to beer body and foam stability but are less fermentable.[123] Mash pH is controlled at 5.2–5.6 to optimize enzyme function, as deviations reduce activity; calcium ions from water treatment aid stability.[125] Traditional step mashing employs sequential temperature rests to target specific enzymes: a protein rest at 50–55°C (122–131°F) activates peptidases for protein breakdown, enhancing clarity in under-modified malts; followed by beta- and alpha-amylase rests.[126] Modern single-infusion mashing, common since the mid-20th century, holds at 65–67°C (149–153°F) for 60 minutes to balance both amylases, suiting well-modified base malts prevalent in contemporary brewing.[121] Conversion completeness is verified via iodine test, where absence of blue-black color indicates full starch breakdown.[127] Mash thickness, typically 3–4 liters of water per kilogram of grist, influences enzyme-substrate interactions; thicker mashes favor beta-amylase for fermentability, while thinner ones enhance alpha-amylase.[125] In adjunct-heavy recipes, exogenous enzymes or cereal cooking gelatinizes starches beforehand for malt enzyme access.[121] Decoction mashing, pulling and boiling a portion of mash before re-adding, intensifies conversion for robust flavors in certain styles like traditional lagers.[126]Wort Separation and Lautering
Wort separation occurs immediately after mashing to isolate the soluble sugars and other extractives in the liquid wort from the insoluble grain solids, primarily spent husks and undegraded proteins.[128] This step aims to achieve high extract recovery while minimizing carryover of solids, colloids, tannins, and oxygen that could affect downstream fermentation and beer clarity.[128] Lautering, the most widely used method internationally, functions as a filtration process through the grain bed, governed by principles akin to Darcy's law, where flow rate depends on permeability, pressure differential, viscosity, and bed depth.[129] The process typically begins with mashout, heating the mash to 168–176°F (76–80°C) to halt enzymatic activity and facilitate flow by reducing viscosity.[129] The mash is then transferred to a lauter tun, a vessel with a slotted false bottom or screens that retain the grain bed while permitting wort drainage; optimal designs feature shallow bed depths and large cross-sectional areas to enhance runoff rates.[129] Initial recirculation (vorlauf) involves collecting the first 2 liters (about 2 quarts) of cloudy runnings and gently repouring them over the bed to clarify the wort by trapping particulates in the husks, continuing until the outflow is free of solids.[130] First runnings are then directed to the kettle, followed by sparging to rinse residual sugars from the grains. Batch sparging entails adding hot water (170°F/77°C) in one or two increments, stirring, resting briefly, and draining, offering simplicity and speed for smaller operations.[130] Fly sparging, conversely, involves continuous addition of sparge water matching the runoff rate, which can extend over an hour but maximizes extraction; it requires halting at a specific gravity of 1.008–1.010 to prevent extracting astringent tannins.[130] Efficiency hinges on factors such as uniform grain milling to balance extract potential with bed permeability, water-to-grist ratios around 7.5 L/kg, and avoiding channeling through even flow distribution.[129] Alternative methods include traditional mash tuns for combined mashing and separation, strainer systems for small-scale brewing, and modern mash filters that press the mash for superior clarity and efficiency in industrial settings, often yielding drier spent grains with minimal moisture.[128] High adjunct mashes (over 50% non-barley grains like wheat or oats) pose challenges by reducing bed stability, potentially requiring adjustments like rice hulls for improved filtration.[129] Common issues, such as stuck sparges, arise from compacted beds or excessive flow rates and can be mitigated by stirring or applying gentle pressure.[129] Overall, lautering targets extract recoveries approaching those of the mash conversion while preserving wort quality for boiling.[128]Boiling, Hopping, and Clarification
The boiling of wort, typically conducted at atmospheric pressure for 60 to 120 minutes depending on batch size and desired evaporation, serves multiple essential functions in brewing.[131][132] It achieves thermal sterilization by denaturing microbial proteins and enzymes, thereby eliminating potential contaminants introduced during mashing or lautering.[132][133] Boiling also inactivates residual malt enzymes such as alpha- and beta-amylases, halting further saccharification and preserving fermentable sugars at their post-mash levels.[133][2] Concurrently, the heat induces coagulation of proteins and tannins into the "hot break" or "kettle trub," a flocculent precipitate that reduces viscosity and removes haze precursors; this process is enhanced by the presence of metal ions like copper from brewing vessels, which catalyze protein denaturation.[132][134] Evaporation during the boil concentrates the wort by 5 to 10 percent of its volume, while volatilizing dimethyl sulfide precursors and other off-flavor compounds, resulting in a pH drop of approximately 0.1 to 0.2 units that further stabilizes the wort.[135][136] Hopping occurs primarily during the boil to solubilize and transform hop compounds for bitterness, flavor, and aroma. Early additions, typically at the start of the boil and lasting 60 minutes or more, promote the thermal isomerization of humulone and other alpha-acids into iso-alpha-acids, which are highly soluble and responsible for beer's enduring bitterness; this reaction follows pseudo-first-order kinetics, achieving 70 to 90 percent conversion under standard conditions of pH 5.0 to 5.5 and boiling temperatures around 100°C.[137][105][138] Mid-boil additions (15 to 30 minutes remaining) balance bitterness with flavor contributions from partially preserved essential oils like myrcene and humulene, while late additions (0 to 5 minutes) or first-wort hopping minimize isomerization to prioritize volatile aroma compounds, as prolonged heat causes their evaporation.[139][140] Hop polyphenol extraction during boiling also contributes to foam stability and astringency, though excessive amounts can lead to harshness.[141] Clarification of the boiled wort targets the removal of trub and suspended solids to prevent carryover into fermentation, which could promote off-flavors or microbial instability. The hot break formed during boiling naturally settles some proteins, but efficiency is improved by kettle fining agents such as carrageenan (extracted from Irish moss), which at concentrations of 4 to 20 grams per hectoliter induces rapid flocculation by binding haze-active polyphenols and proteins, often reducing filtration loads by 20 to 50 percent.[142][143] Post-boil, mechanical methods like whirlpooling exploit centrifugal force in a tangential vessel flow to compact trub into a dense cone, achieving 70 to 90 percent solids separation in 15 to 30 minutes without consumables.[144] These steps precede rapid cooling to avoid cold break formation in the kettle, ensuring clearer wort for pitching; residual turbidity, if unmanaged, correlates with higher levels of oxidative compounds in finished beer.[145][134]Fermentation and Yeast Management
Fermentation in brewing involves the metabolic activity of yeast, primarily species of the genus Saccharomyces, which convert fermentable sugars in the wort into ethanol, carbon dioxide, and flavor compounds. Saccharomyces cerevisiae, a top-fermenting yeast, is used for ales, rising to the surface during fermentation, while Saccharomyces pastorianus, a bottom-fermenter, is employed for lagers, settling at the bottom.[2] This process typically occurs in sealed fermentation vessels after the wort has been cooled to the appropriate pitching temperature, with primary fermentation lasting 3-7 days for ales and 7-14 days for lagers depending on strain and conditions.[2] Effective yeast management begins with proper pitching rates to ensure sufficient cell density for efficient fermentation and to minimize stress-induced off-flavors. Commercial brewers target 5-20 million viable yeast cells per milliliter per degree Plato of wort original gravity, with a common recommendation of 7.5-10 million cells/ml/°P for standard ales to achieve reliable attenuation and flavor consistency.[146] Underpitching can lead to sluggish fermentation, increased ester production, and higher diacetyl levels, while overpitching may reduce yeast vitality over reuse cycles. Yeast is often propagated from lab cultures or harvested from previous batches, with viability assessed via staining or cell counts before pitching.[147] Temperature control is critical during fermentation, as it directly influences yeast metabolism, alcohol yield, and byproduct formation. Ale fermentations are optimally conducted at 18-22°C (64-72°F) to promote clean profiles, whereas lager fermentations require cooler primary temperatures of 8-13°C (46-55°F) to suppress unwanted esters and fusel alcohols, followed by a diacetyl rest at slightly higher temperatures around 15-18°C (59-64°F) for 2-3 days to allow yeast to reabsorb diacetyl.[148] Precise regulation using jacketed tanks or immersion chillers prevents temperature spikes that could cause stuck fermentations or flavor defects.[148] Fermentation progress is monitored through measurements of specific gravity, which declines as sugars are consumed, enabling calculation of apparent attenuation as [(original gravity - final gravity) / original gravity] × 100. Typical attenuation rates range from 70-85% for most ale strains and 75-82% for lager yeasts, with final gravity stabilizing when no further drop occurs over 1-2 days.[149] Additional checks include pH monitoring (dropping from ~5.0 to 4.0-4.5) and sensory evaluation for off-flavors, with tools like hydrometers or refractometers used daily.[150] Post-primary fermentation, yeast management includes cropping—harvesting settled yeast for reuse—and storage under refrigerated conditions to maintain viability for up to 5-10 generations before genetic drift necessitates replacement. Acid washing with phosphoric acid at pH 2.0-2.5 for 1-2 hours can sanitize harvested yeast by reducing bacterial contamination, though it requires careful neutralization and viability checks afterward.[151] Consistent practices in propagation, handling, and recycling ensure economic efficiency and reproducible beer quality across batches.[152]Conditioning, Maturation, and Packaging
Conditioning occurs after primary fermentation, allowing residual yeast to metabolize remaining sugars and reduce off-flavors such as diacetyl through continued metabolic activity.[153] For ales, brewers typically maintain the beer at fermentation temperature for 24-48 hours post-terminal gravity to facilitate diacetyl reduction before cooling.[154] Lagers undergo a diacetyl rest, warming to 10-16°C for 1-2 days at the end of primary fermentation to promote yeast cleanup, followed by cold conditioning.[2] Maturation, or secondary conditioning, involves storing the beer at controlled low temperatures to promote flavor stability, clarity, and colloidal maturation. Ales are often matured at 0-4°C for 3-7 days, while lagers require extended cold storage at near-freezing temperatures (0-4°C or lower) for 1-6 weeks or more, depending on style, to allow haze-forming proteins and polyphenols to precipitate.[2] [155] This phase minimizes oxidative staling compounds like aldehydes, with chemical reactions such as acetaldehyde reduction enhancing smoothness.[156] Cold stabilization may extend to -2°C for 1-2 days to induce permanent chill haze formation, preventing later precipitation in finished beer.[157] Prior to packaging, beer undergoes clarification via fining agents (e.g., isinglass, PVPP) or filtration to remove yeast and particulates, ensuring visual and microbial stability.[153] Carbonation is achieved naturally through refermentation with added sugar or forced via CO2 injection to reach 2.4-2.8 volumes for most styles. Packaging methods include kegging for draft (filled under counter-pressure to maintain CO2), bottling (often with crown caps, primed for conditioning), and canning (aseptic lining prevents metal interaction).[158] Cans have gained prevalence since the 2010s due to superior light protection against skunking from 3-methyl-2-buten-1-thiol formation and recyclability, with over 60% of U.S. craft beer packaged in cans by 2020.[159] Sterile filling and optional pasteurization (e.g., tunnel pasteurization at 60°C for 10 minutes) extend shelf life to 6-12 months under refrigeration.[160]Equipment and Technology
Traditional and Small-Scale Tools
Traditional brewing employed manual tools crafted primarily from wood, copper, and clay, enabling small-scale production reliant on human labor and basic heat sources like open fires. Grain preparation involved hand-operated mills, such as antique flour mills or stone querns, to crush malted barley into grist without powered machinery.[161] Mashing occurred in wooden tuns, often constructed from halved barrels or large vessels insulated with cedar or lined with straw and juniper branches to facilitate filtration. These tuns featured perforated false bottoms or fibrous beds to separate wort from spent grains during lautering, with hot water manually ladled or sparged using perforated buckets in gravity-fed systems.[161][162][161] Boiling utilized hand-hammered copper kettles, valued for their thermal conductivity and resistance to corrosion, positioned over wood fires in floor-mounted setups for small batches up to one-and-a-half barrels. Copper's antimicrobial properties aided in wort sanitation, a practice dating to medieval times when metal pots replaced pottery for heating.[161][163] Fermentation took place in unlined oak barrels or open wooden vats, typically 50-200 liters in capacity, allowing natural yeast inoculation and subtle oxygenation that influenced flavor profiles in styles like farmhouse ales. Wooden vessels, common from ancient Mesopotamian casks to European medieval practices, imparted tannins and required periodic steaming for sanitation due to porosity and microbial harboring.[161][164][165] Cooling relied on manual methods, such as shallow troughs or river immersion for wort, while packaging involved wooden casks or clay amphorae sealed with pitch, emphasizing labor-intensive processes suited to household or village-scale operations before industrialization.[166]Industrial-Scale Machinery
Industrial-scale brewing machinery consists of robust, high-capacity systems engineered for efficient, consistent production of beer in volumes typically exceeding 5,000 liters per batch, enabling breweries to output millions of hectoliters annually.[167] These setups prioritize stainless steel construction, often grades 304 or 316, for durability, hygiene, and corrosion resistance, with integrated automation to minimize human error and optimize resource use.[168] Central to the process is the brewhouse, comprising multi-vessel configurations such as mash tuns, lauter tuns, brew kettles, and whirlpools, which handle enzymatic conversion, wort separation, boiling, and hot trub removal in a continuous or semi-continuous manner.[169] Mash tuns in industrial settings feature steam-jacketed heating and mechanical stirring for uniform temperature control during saccharification, often with capacities scaled to 10-50 hectoliters.[170] Lauter tuns incorporate rakes and false bottoms for efficient grain separation, while brew kettles use internal or external calandria for rapid boiling to achieve wort sterilization and hop isomerization.[171] Whirlpools facilitate solid-liquid separation via centrifugal force, reducing clarification time compared to traditional settling.[172] Fermentation and maturation rely on cylindrical-conical tanks (CCTs) and bright beer tanks, typically ranging from 100 to 1,000 hectoliters, equipped with cooling jackets, pressure valves, and clean-in-place (CIP) systems for temperature regulation and sanitation.[168] These vessels support both ale and lager processes, with automated glycol chillers maintaining precise profiles to control yeast activity and flavor development.[173] Downstream, clarification employs centrifuges or membrane filters for haze removal, bypassing traditional diatomaceous earth in some modern lines to reduce waste.[174] Packaging lines feature high-speed fillers, cappers, labelers, and pasteurizers integrated into automated conveyors, capable of processing thousands of bottles or cans per hour.[172] Overall automation via programmable logic controllers (PLCs) and supervisory control and data acquisition (SCADA) systems monitors parameters like pH, gravity, and temperature in real-time, enhancing scalability and quality control in facilities producing over 1 million hectoliters yearly.[175][176]
Emerging Technologies and Automation
Automation in brewing has advanced through programmable logic controllers (PLCs), sensors, and integrated control systems that optimize processes like mashing, fermentation, and packaging, reducing human error and ensuring batch consistency.[177] [178] These systems enable real-time monitoring of variables such as temperature, pH, and pressure, allowing for precise adjustments that enhance efficiency and product quality in both craft and industrial settings.[179] For instance, automated brewing platforms like those from Brewie control mashing, boiling, and fermentation with minimal operator input, scaling from small batches to larger productions.[180] Artificial intelligence (AI) is increasingly applied to recipe formulation, yeast strain selection, and predictive analytics for fermentation outcomes, accelerating innovation in beer profiles.[181] In 2023, Atwater Brewery released an AI-optimized citrus India pale ale, leveraging algorithms to balance hop bitterness and aroma compounds.[182] By 2025, AI tools have supported development of specialty beers, including low-carb and gluten-free variants, through data-driven enzyme optimization and flavor modeling, driven by consumer demand for functional beverages.[183] AI also aids quality control by analyzing sensor data to detect off-flavors or inconsistencies during production, minimizing waste.[184] Robotics integrated with AI enhances packaging and material handling, performing tasks like palletizing bottles or kegs with high precision and adaptability to varying production volumes.[185] In beverage facilities, these systems enable real-time decision-making for sorting and labeling, reducing labor costs and downtime.[186] Emerging Internet of Things (IoT) networks connect equipment across breweries, facilitating remote diagnostics and predictive maintenance to prevent equipment failures.[187] As of 2025, hybrid automation-AI setups are projected to further consolidate in craft brewing, supporting scalable operations amid market pressures for efficiency.[188]By-Products and Sustainability
Waste Streams and Utilization
Brewers' spent grain (BSG), the fibrous residue from mashing and lautering, constitutes approximately 85% of solid by-products in beer production, generating about 20 kg of wet BSG per hectoliter of beer brewed.[189] Globally, this yields around 36.4 million tonnes annually, based on production volumes exceeding 1.9 billion hectoliters.[190] BSG comprises lignocellulosic material with 15-25% protein, 15-20% fiber, and residual sugars, making it nutrient-dense but prone to rapid spoilage due to high moisture content (70-80%).[191] Other solid wastes include spent yeast, recovered post-fermentation at 2-5 kg per hectoliter, which retains viable cells (up to 60% after 24 hours) and proteins suitable for repurposing, and trub—protein-polyphenol complexes with hop residues—from boiling and whirlpooling, amounting to 1-2 kg per hectoliter.[192] Liquid effluents, primarily from cleaning and cooling, total 4-10 hectoliters per hectoliter of beer, characterized by high biochemical oxygen demand (BOD) from organic loads like sugars and yeast.[193] Gaseous by-products, notably CO2 from fermentation (about 0.1-0.2 kg per hectoliter), are often vented but increasingly captured.[194] Utilization of BSG focuses on animal feed, where wet forms feed ruminants and dried variants suit monogastrics, leveraging its digestibility and protein value to offset disposal costs.[195] Biotechnological conversions extract proteins for food additives, fibers for baking, or ferment into bioethanol and biogas via anaerobic digestion, achieving yields of 0.2-0.3 L ethanol per kg dry BSG.[196] Spent yeast is propagated for repitching in subsequent brews or processed into feeds, though improper disposal risks septic overload in wastewater systems due to its oxygen demand.[197] Trub finds similar applications in biogas production or as fertilizer after stabilization. Wastewater management employs screening to capture solids like grains and yeast before anaerobic or aerobic treatment, reducing BOD by 90% in integrated systems and enabling water recycling.[198] CO2 recovery via scrubbers supports carbonation or sale to food industries, minimizing emissions.[199] These strategies align with circular economy principles, converting wastes into value-added products and averting landfill use, though challenges persist in scaling extraction technologies for economic viability.[200]Environmental Impacts and Mitigation
The brewing industry generates notable environmental impacts through resource-intensive processes, particularly in water and energy use, alongside emissions and effluent challenges. Water consumption averages 4 to 7 liters per liter of beer produced, driven by mashing, lautering, cooling, and sanitation requirements, with craft operations often exceeding this due to smaller-scale inefficiencies.[201] [202] Wastewater from these activities carries high organic loads, typically featuring biological oxygen demand (BOD) of 1,200–3,600 mg/L and chemical oxygen demand (COD) of 2,000–6,000 mg/L, which can strain municipal treatment systems if not pretreated due to rapid microbial activity and potential for oxygen depletion in receiving waters.[203] Energy demands center on thermal inputs for wort boiling and steam generation, totaling 150–180 MJ per hectoliter, plus 8–16 kWh electricity per hectoliter for pumps, refrigeration, and controls, often reliant on fossil fuels that contribute to an average of 0.39 kg CO₂ equivalent emissions per liter of beer.[204] [205] These impacts arise causally from the thermodynamics of extraction and sterilization—high temperatures necessitate energy for phase changes and pathogen control—compounded by the dilutional nature of beer (over 90% water), which amplifies upstream resource pulls. Empirical benchmarking shows industry-wide progress, with water use intensity declining 8% from 2017 to 2022 across reporting breweries, yet absolute volumes remain substantial given global production exceeding 1.9 billion hectoliters annually.[206] Emissions variability stems from fuel choices and supply chain factors, with life-cycle assessments indicating ranges up to 2.59 kg CO₂e per liter in less efficient setups, underscoring the need for site-specific scrutiny over generalized industry averages.[207] Mitigation strategies emphasize process optimization and circular approaches to curb these effects without compromising product quality. Water efficiency has advanced via closed-loop cooling, membrane filtration for reuse, and low-flow cleaning-in-place systems, enabling reductions to 3 hectoliters water per hectoliter beer in optimized facilities like those benchmarked in 2023.[208] [209] Energy savings derive from heat exchangers recovering up to 90% of boiler flue gases and electrification of kettles using resistive or inductive heating, potentially halving thermal demands to below 4 kWh per hectoliter while integrating renewables like solar or biogas.[210] [211] For effluents, anaerobic digesters convert organic waste into methane for on-site power, achieving 80–95% BOD removal and offsetting 20–30% of facility energy needs, as demonstrated in installations reducing discharge loads to compliant levels.[212] Broader tactics include ingredient sourcing from low-input agriculture to trim scope 3 emissions and packaging shifts to lightweight aluminum over glass, which cuts transport-related CO₂ by factors of 3–5 per unit.[213] These measures, validated through industry consortia like the Beverage Industry Environmental Roundtable, yield verifiable reductions but require capital investment, with payback periods of 2–5 years in high-volume operations.[206]Brewing Industry
Global Production and Economics
Global beer production totaled 1.875 billion hectoliters in 2023, reflecting a marginal decline of 0.3% from the previous year.[214] China dominated output with 359 million hectoliters, accounting for roughly 19% of the global total, followed by the United States at 193 million hectoliters and Brazil at 149 million hectoliters.[215] [216]| Rank | Country | Production (million hectoliters, 2023) |
|---|---|---|
| 1 | China | 359 |
| 2 | United States | 193 |
| 3 | Brazil | 149 |
| 4 | Mexico | (Data indicates significant volume, exact figure ~140 based on regional trends)[216] |
Craft Brewing Movement and Market Dynamics
The craft brewing movement originated in the United States in the mid-1960s as a response to the homogenization of beer production by large industrial brewers offering uniform, low-flavor lagers. Fritz Maytag's acquisition and revival of Anchor Brewing Company in 1965 marked an early milestone, focusing on traditional steam-beer methods and ingredient quality to differentiate from mass-market offerings.[44] The federal legalization of homebrewing on January 1, 1979, catalyzed broader participation, enabling experimentation that transitioned into commercial ventures like microbreweries and brewpubs during the 1980s.[224] This period saw the term "craft brewery" emerge, defined initially in 1987 by Charlie Papazian as involving manual arts and skills rather than automated processes.[225] The Brewers Association formalized the craft brewer definition as small (annual production of 6 million barrels or less), independent (≤25% owned or controlled by a non-craft brewer), and traditional (beer constituting ≥75% of total beverage volume, using primarily traditional or innovative ingredients).[226] This framework excludes larger entities engaging in substantial non-beer production or those with significant macro-brewer ownership, aiming to delineate operations prioritizing brewing integrity over scale.[227] In the U.S., craft breweries proliferated from fewer than 100 in 1980 to a peak of over 9,700 operating by 2023, but 2024 recorded the first net decline since 2005, with 529 closures against 430 openings, resulting in about 9,600-9,700 total.[228] [229] Craft volume share hovered around 12-13% of the U.S. beer market, but sales dropped 4% in 2024 amid overall beer production declining 1%.[230] Globally, craft segments expanded unevenly, with markets projected to grow from USD 142.6 billion in 2024 to USD 329.7 billion by 2033, driven by demand in emerging regions like Asia, though mature markets faced saturation.[231] Market dynamics reflect maturation and contraction pressures: consolidation via acquisitions by macro-brewers has reduced independence, with larger players leveraging distribution advantages to entrench market share.[232] Rising input costs, elevated interest rates, and shifting consumer behaviors—favoring seltzers, spirits, or low/no-alcohol options—exacerbate challenges for small operators reliant on taprooms and local sales.[233] [234] Distribution consolidation limits shelf space for independents, while overcapacity from pandemic-era expansions contributes to closures.[235] Brewers Association data indicates 2025 uncertainties, including potential tariffs and cost inflation, may intensify these trends, prompting strategic shifts toward efficiency, localization, and non-beer diversification.[228] [236]Regulatory Frameworks and Trade
Regulatory frameworks for brewing encompass licensing, production standards, labeling, taxation, and safety requirements enforced by national authorities to mitigate health risks, ensure fiscal revenue, and protect consumers from adulteration. In the United States, the Alcohol and Tobacco Tax and Trade Bureau (TTB) mandates that breweries obtain a brewer's notice under 27 U.S.C. Chapter 51 and 27 CFR Part 25, covering brewery location, equipment specifications, operational procedures, and meticulous record-keeping for production volumes and tax liabilities.[237] Federal excise taxes apply at $18 per barrel for beer not exceeding 6% alcohol by volume (ABV), escalating to $3.50 per barrel for higher strengths up to 14% ABV, with small brewers eligible for reduced rates under the Craft Beverage Modernization and Tax Reform Act of 2017.[237] Labeling requirements include producer details, alcohol content, and net contents, but exclude mandatory nutrition facts or health warnings unless states impose them, though TTB proposed standard serving sizes of 12 ounces for malt beverages under 7% ABV in 2024.[238] [239] In the European Union, beer production falls under general food safety regulations like Commission Regulation (EU) 2023/915 for contaminants, with alcoholic beverages exceeding 1.2% ABV exempt from obligatory ingredient lists and nutrition declarations per Regulation (EU) 1169/2011, prioritizing allergen disclosures instead.[240] [241] Member states must impose minimum excise duties of €1.87 per hectoliter per degree Plato, though rates vary widely—Finland's reaching €31.42 and Hungary's €3.30 as of 2025—often structured by alcohol content to discourage higher-strength products.[242] Germany's Reinheitsgebot, enacted in Bavaria in 1516 to safeguard bread supplies and beer quality by restricting ingredients to malted barley, hops, yeast, and water (with wheat allowances added later), ceased being enforceable law following a 1987 European Court of Justice ruling against trade barriers, yet persists as a cultural and marketing standard for over 90% of domestic beers.[243] Brewing licenses emphasize hygiene and traceability, with low- and no-alcohol beers facing definitional ambiguities across states, allowing up to 0.5% ABV for "alcohol-free" claims in some jurisdictions.[244] Internationally, trade in beer adheres to World Trade Organization (WTO) principles under the General Agreement on Tariffs and Trade (GATT) and Agreement on Technical Barriers to Trade (TBT), prohibiting discriminatory tariffs and non-tariff barriers while permitting excise taxes as fiscal tools.[245] [246] Exports from the U.S. require TTB certification of tax payment or drawback claims, compliance with destination-country labeling (e.g., metric volumes in the EU), and navigation of bilateral agreements like the USMCA, which exempts certain North American ingredient imports from tariffs.[247] [248] As of 2025, retaliatory tariffs burden EU beer exports to the U.S. with a 15% duty plus 50% on canned formats, potentially reshaping import shares dominated by Mexico (exempt under USMCA) and Europe, while U.S. brewers import over 20% of malt and hops, exposing supply chains to protectionist policies that elevate costs without proportional domestic substitution.[249] [250] Global trade volumes, valued at approximately $100 billion annually pre-tariff escalations, face disruptions from such measures, with WTO disputes historically challenging state monopolies and discriminatory distribution laws in markets like Canada and Japan.[251] [252] Emerging markets impose additional hurdles, including advertising bans and import quotas, as seen in Middle Eastern countries restricting non-Muslim consumption despite pockets of liberalization in the UAE.[253]Health and Societal Impacts
Nutritional Composition and Empirical Effects
Beer, the principal output of brewing processes, exhibits a nutritional profile dominated by ethanol and carbohydrates, with contributions from proteins, micronutrients, and bioactive compounds varying by style, fermentation, and ingredients. A standard 355 ml (12 oz) serving of lager typically provides 153 calories, comprising 13.6 g carbohydrates (mostly fermentable sugars converted to alcohol), 1.6 g protein from malt-derived amino acids, and 14 g ethanol, which accounts for about 50% of caloric content, alongside negligible fat.[254] Darker or higher-gravity beers, such as stouts, contain elevated carbohydrates (up to 20 g per serving) and calories (200+), while light variants reduce these to around 100 calories and 6 g carbs through attenuated fermentation.[255] Micronutrients in beer include B vitamins (e.g., 10% daily value of niacin and riboflavin per serving from yeast and malt), folate (up to 5-10% DV), and minerals like magnesium (5% DV), potassium (2-3% DV), and silicon (derived from barley husks, averaging 10-20 mg/L).[256] [257] Beer also supplies soluble fiber, primarily arabinoxylan from barley, at 1-2 g per liter, supporting gut microbiota fermentation. Polyphenols, including phenolic acids (e.g., ferulic and caffeic acids) from hops and malt, total 100-300 mg/L, conferring antioxidant capacity equivalent to red wine on a per-volume basis, though distinct in composition (e.g., higher xanthohumol from hops).[258] [259]| Nutrient (per 355 ml standard lager) | Amount | % Daily Value (approx.) |
|---|---|---|
| Calories | 153 | - |
| Carbohydrates | 13.6 g | 5% |
| Protein | 1.6 g | 3% |
| Ethanol | 14 g | - |
| Niacin | 1.2 mg | 10% |
| Folate | 20 μg | 5% |
| Silicon | ~5 mg | - |
| Total Polyphenols | 150-250 mg | - |
