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Cannabis cultivation
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The cultivation of cannabis is the production of cannabis infructescences ("buds" or "leaves"). Cultivation techniques for other purposes (such as hemp production) differ.
In the United States, all cannabis products in a regulated market must be grown in the state where they are sold because federal law continues to ban interstate cannabis sales. Most regulated cannabis is grown indoors.[1]
Occupational diseases, including asthma, are an emerging concern in the rapidly expanding U.S. cannabis industry. Cannabis cultivation and processing technicians may be exposed to numerous respiratory hazards, e.g. organic particulate matter and dust from ground cannabis flower, mold, bacterial endotoxins, and pesticides. Employees exposed to ground cannabis without adequate controls are at risk of developing occupational asthma which can be fatal.[2][3][4]
Botany
[edit]Cannabis belongs to the genus Cannabis in the family Cannabaceae. It may include three species, Cannabis indica, C. sativa, and C. ruderalis (APG II system), or one variable species.[5][additional citation(s) needed] It is typically a dioecious (each individual is either male or female) annual plant.[6][7][8]
C. sativa and C. indica generally grow tall, with some varieties reaching 4 metres or 13 feet. Female plants produce tetrahydrocannabinol (THC) (up to 31% by weight) as the season changes from summer to autumn. C. ruderalis is very short, produces only trace amounts of THC, but is very rich in cannabidiol (CBD) an antagonist to THC, which may be 40% of the cannabinoids in a plant. C. ruderalis flowers independently of the photoperiod with the main factor for flowering being the age of the individual plants.[9] However, commercial cross-bred hybrids containing both ruderalis, indica and/or sativa genes exist (usually called autoflowering).
Cultivation requirements
[edit]Cannabis needs certain conditions to flourish.
Growth medium
[edit]Soil is required, except for cannabis grown with hydroponics or aeroponics.
- Sufficient nutrients — commercial potting soils usually indicate this as "N-P-K = x%-y%-z%". This indicates the percentages of fundamental nutritional elements, i.e., nitrogen, phosphorus and potassium. Nutrients are often provided to the soil via fertilizers but such practice requires caution.[10]
- A soil pH between 5.8 and 6.5. This value can be adjusted – see soil pH. Commercial fertilizers tend to make the soil more acidic, although manure has a less pronounced effect.[11]
Warmth
[edit]The optimal day temperature range for cannabis is 24 to 30 °C (75 to 86 °F).[12] Temperatures above 31 °C (88 °F) and below 15.5 °C (60 °F) seem to decrease THC potency and slow growth. At 13 °C (55 °F) the plant undergoes a mild shock, though some strains withstand frost temporarily.[13][14][15]
Light
[edit]Sun light or grow light can be used.
Under artificial light, the plant typically remains under a regime of 16–24 hours of light and 0–8 hours of darkness from the germination until flowering, with longer light periods being conducive to vegetative growth, and longer dark periods being conducive to flowering. However, generally cannabis only requires thirteen hours of continuous light to remain in the vegetative stage.[16] The 'Gas Lantern Routine' is an alternate lighting schedule that has proven to be successful for growing cannabis, while saving a significant amount of energy.[17][18][19] For optimal health, cannabis plants require a period of light and a period of dark.[20] It has been suggested that, when subjected to a regimen of constant light without a dark period, cannabis begins to show signs of decreased photosynthetic response, lack of vigor, and an overall decrease in vascular development. Typically, flowering is induced by providing at least 12 hours per day of complete darkness. Flowering in cannabis is triggered by a hormonal reaction within the plant that is initiated by an increase in length of its dark cycle, i.e. the plant needs sufficient prolonged darkness for bract/bracteole development[21] (flowering) to begin. Some Indica varieties require as little as 8 hours of dark to begin flowering, whereas some Sativa varieties require up to 13 hours.
Water
[edit]Watering frequency and amount is determined by many factors, including temperature and light, the age, size and stage of growth[22] of the plant and the medium's ability to retain water. A conspicuous sign of water problems is the wilting of leaves.[23] Giving too much water can kill cannabis plants if the growing medium gets over-saturated. This is mainly due to oxygen not being able to enter the root system.[24] Anaerobic bacteria start to accumulate due to waterlogged, stale conditions. They begin to consume plant roots, beneficial (aerobic) bacteria, as well as nutrients and fertilizer.[24] When using soil as a growth medium, the soil should be allowed to dry down adequately before re-watering.[24]
Humidity
[edit]Humidity is an important part of plant growth. Dry conditions slow the rate of photosynthesis.[25] Ideal levels of humidity for optimal growth are 40–60% RH.

Nutrients
[edit]Nutrients are taken up from the soil by roots. Nutrient soil amendments (fertilizers) are added when the soil nutrients are depleted. Fertilizers can be chemical or organic, liquid or powder, and usually contain a mixture of ingredients. Commercial fertilizers indicate the levels of NPK (nitrogen, phosphorus, and potassium). In general, cannabis needs more N than P and K during all life phases. The presence of secondary nutrients (calcium, magnesium, sulfur) is recommended. Micronutrients (e.g. iron, boron, chlorine, manganese, copper, zinc, molybdenum) rarely manifest as deficiencies.
Because cannabis' nutrient needs vary widely depending on the variety, they are usually determined by trial and error and fertilizers are applied sparingly to avoid burning the plant.[27]
Stages of development
[edit]This section needs additional citations for verification. (March 2021) |
Germination
[edit]
Germination is the process by which a seed sprouts and a root emerges. Germination in cannabis can occur in as little as twelve hours or can take as long as eight days, depending on the cultivar and environmental conditions. Warmth, darkness, and moisture initiate metabolic processes such as the activation of hormones that trigger the expansion of the embryo within the seed. Then the seed coat cracks open and a small embryonic root emerges and begins growing downward (because of gravitropism), if placed in a proper growing medium. Soon (after 2–4 days) the root is anchored and two oval cotyledons (sometimes called "false leaves" or "seed leaves") emerge in search of light and the remains of the seed shell are pushed away. This marks the beginning of the seedling stage.
Peat pellets are often used as a germinating medium because the saturated pellets with their seedlings can be planted directly into the intended growing medium with a minimum of shock to the plant.
- Scarification (artificial), example: Soaking the seeds in boiled water that is cooled down.
- Stratification (natural simulation), example: Soaking seeds either between wet paper towels, in a cup of water at room temperature, in wet peat pellets, or directly in potting soil.
Imbibition
[edit]Imbibition of water through the membrane of the seed shell is the first step in the germination process for cannabis. Dry cannabis seeds are floating, a convenient way to verify a successful imbibition is to put them in water until they sink, which takes about six hours when submerged in an infuser, and about 12 hours when they are floating on water. Seeds that keep floating need scarification in order to absorb water.
Seedling
[edit]
The seedling stage begins when the seed coat splits open and exposes the root and cotyledons. It lasts from 1 to 4 weeks, and is the period of greatest vulnerability in the life cycle of the plant, requiring moderate humidity levels, medium to high light intensity, and adequate but not excessive soil moisture.
Most indoor growers use compact fluorescent or T5 fluorescent lights during this stage as they produce little heat. High-pressure sodium and metal halide lights produce large amounts of radiant heat and increase the rate of transpiration in the plant which can quickly dry out seedlings with their small root systems.
Vegetative
[edit]
Duration: 1–2 months indoors. In this stage the plant needs a significant amount of light and nutrients, depending on the genetics of the particular plant. It continues to grow vertically and produce new leaves. The sex is starting to reveal itself, which is a sign that the next stage begins. Concurrently the root system expands downwards in search of more water and food.
When the plant possesses seven sets of true leaves and the 8th is barely visible in the center of the growth tip, or shoot apical meristem (SAM), the plant has entered the vegetative phase of growth. During the vegetative phase, the plant directs its energy resources primarily to the growth of leaves, stems, and roots. A strong root system is required for strong floral development. A plant needs 1 or 2 months to mature before blooming. The plant is ready when it has revealed its sex. Plant size is a good indicator of sex. Females tend to be shorter and branchier due to their raceme type inflorescence than males, whose flowers grow in panicles. The males are then usually culled when they are identified, so that the females will not be pollinated, thus producing parthenocarpic fruits (popularly called "sinsemilla", meaning "without seed").
During the vegetative phase, cultivators generally employ an 18- to 24-hour photoperiod because the plants grow more quickly if they receive more light, although a warmer and cooler periods are required for optimal health. Although no dark period is required, there is debate among cultivators as to whether a dark period is beneficial, and many continue to employ a dark period. Energy savings often support using a dark period, as plants undergo late day decline and therefore lighting during the late night hours is less effective.
The amount of time to grow a cannabis plant indoors in the vegetative stage depends on the size of the flower, the light used, the size of the space, and how many plants are intended to flower at once, and how big the strain gets in "the stretch" (i.e., the first two weeks of flowering).
Cannabis cultivators employ fertilizers high in nitrogen and potassium during the vegetative stage, as well as a complete micronutrient fertilizer. The strength of the fertilizer is gradually increased as the plants grow and become more hardy.
Advanced cultivation methods include:
- training and trellising techniques such as Screen of Green (also known as SCROG), Sea of Green (also known as SOG) "Super cropping" and LST super cropping; and entire systems and methods such as the NIMBY no-dump method, Hempy Bucket, and the Krusty Freedom Bucket methods. Research into the production of cannabis for the drug Marinol and other more profitable and marketable forms of cannabis-based medicines has further pushed the envelope of cannabis cultivation in all forms of laboratory, both public and private.
- using a water or air-based growth medium (known as hydroponics and aeroponics respectively)
- the use of homemade, organic composted fertilizers
The emphasis on advanced cultivation techniques, as well as the availability of hybrid strains (with names like Northern Lights, Master Kush, NYC Diesel), is believed to be a factor in the increase in the overall quality and variety of commercially available cannabis over the past few decades. The Internet in particular has brought together widely diverse genetics from around the world through trading and purchasing. However, well-grown heirloom strains (e.g. island sweet skunk, fruity Thai etc.) are used to produce 1 gram; 1/28 oz per watt (g/W) harvest.
Pre-flowering
[edit]
Also called the stretch, this takes one day to two weeks. Most plants spend 10–14 days in this period after switching the light cycle to 12 hours of darkness. Plant development increases dramatically, with the plant doubling or more in size. (See reproductive development below.) Production of more branches and nodes occurs during this stage, as the structure for flowering grows. The plant starts to develop bracts/bracteoles where the branches meet the stem (nodes). Pre-flowering indicates the plant is ready to flower.
Flowering and fruition
[edit]
The flowering phase varies from about 6 to 12 weeks for pure indicas with their shorter flowering time than pure sativas. Mixed indica/sativa strains have an intermediate flowering time. The sex is clearly revealed in the first phase, the actual flowering. Males produce little ball-like flowers clustered together like grapes called panicles. Most plants (except auto flowering strains that flower independently of photoperiod) begin to flower under diminishing light. In nature, cannabis plants sense the forthcoming winter as the Earth revolves about the Sun and daylight reduces in duration (see also season). The next phase consists in the fruition (or fruiting): the females inflorescences that were not pollinated (i.e.: fertilized by male pollen) start to produce infructescences that contain sticky white resin-containing glands (or trichomes) in a final attempt for pollination by windborne male pollen. The trichomes produce resins that contain the largest amounts of THC and CBN, the two main psychoactive substances. Fertilized females continue to produce resinous trichomes but more plant energy is consumed by the production of seeds, which can be half the mass of a fertilized bract; thus, to maximize resin by weight, infertile cultivation is preferred.
Inflorescences that produce no seeds are called sin semilla (which translates to "without seeds" in Spanish, and is often misspelled as one word). Potent sin semilla is especially important to medical users, to minimize the amount of cannabis they must consume to be afforded relief. Cannabis with seeds is generally considered to be of inferior quality and/or grown with inferior technique.

Cannabis grown is induced into flowering by decreasing its photoperiod to at least 10 hours of darkness per day. In order to initiate a flowering response, the number of hours of darkness must exceed a critical point. Generally the more hours of darkness each day, the shorter the overall flowering period but the lower the yield. Conversely, the fewer hours of darkness each day, the longer the overall flowering period and the higher the yield. Traditionally, most growers change their plants lighting cycle to 12 hours on and 12 hours off since this works as a happy medium to which most strains respond well. This change in photoperiod mimics the plant's natural outdoor cycle, with up to 18 hours of light per day in the summer and down to less than 12 hours of light in fall and winter.[citation needed] Some 'semi-autoflowering' strains that have been bred exclusively for outdoor use, particularly in outdoor climates such as that of the UK, will start flowering with as much as 16–17 hours of light per day. Usually they can start flowering in July and finish far earlier than other strains, particularly those that haven't been bred as outdoor strains. Semi-autoflowering strains can be harvested before the weather in northern latitudes becomes very wet and cold (generally October), whereas other strains are just finishing flowering, and may suffer from botrytis (grey mold) caused by wet weather. Alternatively growers may artificially induce the flowering period during the warmer months by blacking out the plants for 12 hours a day i.e. by covering the plants with black plastic for example, which excludes all light during this period so the plant can flower even during long days.
Although the flowering hormone in most plants (including cannabis) is present during all phases of growth, it is inhibited by exposure to light. To induce flowering, the plant must be subject to at least 8 hours of darkness per day; this number is very strain-specific and most growers use 12 hours of darkness.
Flowers from certain plants (e.g. cannabis) are called bract/bracteole, and are (with cannabis) the most prized part of the plant. During the late period, the bract/bracteole are easily visible to the naked eye. Bract/bracteole development begins approximately 1–2 weeks after the photoperiod is reduced. In the first weeks of flowering a plant usually doubles in size and can triple. Bract/bracteole development ends around 5 weeks into flowering and is followed by a period of bract/bracteole "swelling". During this time the buds greatly increase in weight and size.[13][14]
Outdoor cannabis cultivation
[edit]

Cannabis can be grown outdoors, either on natural soil or in pots of pre-made or commercial soil. Some strains perform better than others in outdoor settings, an attribute that depends on different conditions, variables and aspects. Outdoor marijuana strains, like most other strains, can be bought in numerous locations and over a hundred different cannabis strains that are bred for outdoor growing exist—many of these outdoor cannabis seeds are simply copies of other pre-existent strains or seeds with different names and descriptions.[28]
To generate optimum quantities of THC-containing resin, the plant needs a fertile soil and long hours of daylight. This means THC production for outdoor growth occurs optimally anywhere within 35° of the equator. Typical growing regions include Mexico, Nepal, Northern India, many parts of Africa, Afghanistan, the United States and Australia.
In most places of the subtropics, cannabis is germinated from late spring to early summer and harvested from late summer to early autumn.

Outdoor cultivation is common in both rural and urban areas. Outdoor cultivators tend to grow indica-based strains because of its heavy yields, quick maturing time, and short stature. Some growers prefer sativa because of its clear-headed (cerebral) high[citation needed], better response to sunlight, and lower odor emissions. Growers cultivate on their own property or practice guerrilla farming i.e. to plant cannabis in remote areas such as forest clearings or mountain cliffs which they rarely visit. However, such a method is prone to theft – so much so that some growers even attach pots to trees to decrease this possibility.[29] Guerrilla growing has given birth to the activist movement Operation Overgrow, where the plant is grown with the explicit purpose of introducing the cannabis plant into the natural ecosystem.
For outdoor cultivation, growers choose areas that receive twelve hours or more of sunlight a day. In the Northern Hemisphere, growers typically plant seeds in mid-April, late May, or early June to provide plants a full four to nine months of growth. Harvest is usually between mid-September and early October. In North America, northern locations are preferred (North Coast of California and British Columbia being particularly notable), but southern locations (such as Maui, Hawaii) are also known to be good producers.[30]
Regulations permitting outdoor cultivation of cannabis vary from state to state in the US. Illinois bans outdoor cultivation and requires growing in an enclosed and locked facility. Other states, like Michigan or Alaska, leave decisions about outdoor growing up to local governments. California, Vermont, and Massachusetts provide licenses for outdoor cultivation, often tiered based on size of operation. Vermont and Alaska also impose security restrictions on outdoor operations such as video surveillance, lack of visibility from roadways, and physical barriers.[31]
Where local laws do not permit growing cannabis, cultivators sometimes grow in forests or rugged and rural areas where the local population is unlikely to find the crop. Cannabis is also grown hidden by a crop that is taller, such as maize. This is reported by the United States government to be common in the midwestern states.[citation needed] Bamboo and elderberry are also used as camouflage companion plants.[citation needed]
Some government agencies, including the Drug Enforcement Administration (DEA), have claimed that in State and National Parks people have been injured by cannabis farmers protecting their crops using booby traps; no arrests or convictions for this had been made as of 2007[update].[citation needed]
Indoor cannabis cultivation
[edit]
Cannabis can be grown indoors in a soil-like medium under artificial light, adding plants are given water. Cultivating cannabis indoors is more complicated and expensive than growing outdoors, but it allows the cultivator complete control over the growing environment. Plants of any type can be grown faster indoors than out due to 24-hour light, additional atmospheric CO2, and controlled humidity which allows freer CO2 respiration.
Plants can also be grown indoors through the use of hydroponics.
To grow plants indoors, a growing medium (e.g. soil or growing substrate), water, nutrients, light and air need to be supplied to the plant (with the exception of aeroponic cultivation, in which case a growing medium is not required).
Supply of light
[edit]There are several different plant grow lights available. Currently the best source of light for cannabis remains to be metal halide or ceramic metal halide in the range of 3-4000k. Cannabis plants also require both dark and light photoperiods, so the lights need a timer to switch them on and off at set intervals. The optimum photoperiod depends on each plant (some prefer long days and short nights and others preferring the opposite, or something in between).

Recent advancements in LED technology have allowed for diodes that emit enough energy for cannabis cultivation. These diodes can emit light in a specific nanometer range, allowing for total control over the spectrum of the light. LEDs are able to produce all of their light in the photosynthetically active range (PAR) of the spectrum.
Reflectors are often used in the lamps to maximize light efficiency.[32] At a medium growing density of 15 plants and 430 watts per square meter, electricity costs are not insignificant.[33] Plants or lights are moved as close together as possible so that they receive equal lighting and that all light coming from the lamps falls upon the plants. Maximum efficiency can be obtained by creating a slightly concave canopy such that the periphery and centre of the canopy are both at the optimum distance from the light source. Often, the distance between lamp and plant is in the range of 0.6 m (2 ft) with high pressure sodium lamps, to 10 cm (4 in) with other lamps, such as compact, large and high-output fluorescent lamps. With proper cooling any light type can be moved extremely close to plants to combat the inverse square law, but there are reasons to keep some distance from the canopy regardless of heat concerns; excessive light can cause bleaching of the plant material and the total canopy area contacted by light is decreased as the source is moved closer. Maximum efficiency should be obtained by maximizing the average light intensity (measured in PAR watts) per square foot times the number of square feet of plant matter contacted. Some cannabis cultivators cover the walls of their grow-room with some type of reflective material (often Mylar or Visqueen), or alternatively, white paint to maximize efficiency.
One commonly used covering is 150 μm (6 mils) PVC plastic sheeting that is white on one side and black on the other. The plastic is installed with the white side facing into the room to reflect light, and the black facing the wall, to reduce fungus and mold growth. Another common covering is flat white paint, with a high titanium dioxide content to maximize reflectivity. Some growers consider Mylar sheeting to be very effective when it lines grow room walls, along with Astrofoil (which also reflects heat), and Foylon (a foil-laminated, reinforced fabric).
Control of the atmosphere
[edit]When growing indoors, the cultivator should maintain as close to an ideal atmosphere inside the grow-room as possible. The air temperature should be maintained within a specific range, typically with deviations no larger than 10 °C (18 °F) with a cooler night and warmer day. Adequate levels of CO2 must be maintained for the plants to grow efficiently. It is also important to promote vigorous air circulation within the grow room, which is usually accomplished by mounting an extraction fan and one or more oscillating fans. Power supplies that can be unscrewed from lamp luminaires can be placed outside grow tents to reduce the temperature a bit.
Assuming adequate light and nutrients are available to plants, the limiting factor in plant growth is the level of carbon dioxide (CO2). Ways of increasing carbon dioxide levels in the grow-room include: bottled carbon dioxide, carbon dioxide generators, a milk jug and yeast solution (in which yeast grows in a container thereby emitting CO2), a baking soda and vinegar mixture in a container, or dry ice.[citation needed]
Certain plants (e.g. most strains of cannabis) emit a distinctive odor during their reproductive phase. This presents difficulties to those who are cultivating in places where it is illegal, or for growers who may prefer discretion for other reasons. The most common way of eliminating odor is by pulling odorous air through a carbon filter. Many cultivators simply attach a large carbon filter to their air extraction system, thereby filtering any smell before the air is expelled from the grow-room. Another way of eliminating odor is by installing an ozone generator in the extraction ducting. The air is forced past the ozone generator by the extraction fan, and the odorous air is neutralized as it mixes with the ozone; however the cultivator must ensure that the air is thoroughly mixed before it is expelled outside, lest some odor escape. Care must be taken to prevent excessive ozone concentrations in the garden itself, or where it might be inhaled by the grower or their family. Ozone itself has a distinctive smell and is harmful to living things, although the molecule breaks down quickly (20 minutes to an hour) in atmospheric conditions.
Indoors there are numerous configurations that people utilize to grow quality cannabis. Some growers will convert an entire room or closet, making it devoted to growing cannabis. A relatively new configuration involves the use of grow tents. These are plastic or metal framed tents which are covered in a strong flexible reflective plastic and have light proof zipper doors. Tents come in all sizes and many already have holes for exhaust fans/ducting as well as mounts for HID lamps.
Some growers will construct grow cabinets made out of an old refrigerator, cabinet, dresser drawer or similar.
Popularity and extent
[edit]
Indoor growing became increasingly common when equipment, seeds and instructions on how to cultivate became widely available. So-called grow-ops (growing operations, often located in grow houses) are seen by many marijuana enthusiasts as a much cheaper way to gain a steady, higher-quality supply of cannabis. On a larger scale they have proven a viable commercial venture, with some law enforcement agencies finding grow-ops large enough to yield several kilograms (pounds) of cannabis. More expansive grow-ops are generally more susceptible to detection than smaller operations.
In the UK, so much cannabis is grown in illegal facilities that the UK is an exporter of cannabis. After cannabis as a drug was rescheduled as a Class B drug in 2008 (see below), more people started reporting on their suspicions of illegal operations and in 2009-2010 almost 7000 illegal facilities were found by police in one year. Vietnamese teenagers are trafficked to the United Kingdom and forced to work in these facilities. When police raid them, trafficked victims are typically sent to prison.[34][35]
Because individual grow light power generally ranges from 250 watts to 1000 watts or more and because the lights remain lit for a long time each day, differences in utility bill costs are a significant security issue. Power companies inform law enforcement if they see a significant increase in power usage relative to a household's previous electricity costs or if power is being stolen by bypassing the meter. Employing energy saving methods is a common way to alleviate this, for instance; switching off light bulbs when leaving rooms, purchasing energy efficient appliances, using TVs or computers less, buying lower power light bulbs and so forth.
Some plants (e.g. cultivars of C. sativa subsp. indica), can give off strong odors as they grow, resulting in detection of illegal growing operations. Growers frequently use carbon scrubbers and ventilation to control odors. This typically involves forcing air from the grow room through a device containing activated carbon, then venting it outdoors. Others use an ozone generator. Ozone reacts with odor molecules in the air, permanently eliminating them. However, ozone can build up to levels that may be hazardous both for grower and plant. As a last resort, keeping windows firmly shut and using strong air fresheners can control smells. Checking outside to see if any smells are emanating from indoors is often a necessary precaution, as many growers become acclimated to the smell, and fail to realize just how pervasive the odor may be. Many store plants in more isolated areas such as a basement or attic to prevent smell detection. Another less common solution is to simply grow a strain with a weaker odor.
Storing plants and lights away from windows and areas that visitor may see is also common, as is keeping the plants in an attic or basement. Some growers, finding this impractical, may cover windows with light-resistant materials. This can solve the problem of escaping bright light but may arouse suspicion amongst neighbors and local residents.
Many cultivators face the risk of fire. Fires normally originate from faulty electrical equipment or wiring. Shoddy fixtures and sockets, improperly grounded equipment, and overloaded circuit breakers are some of the most prevalent causes. Because of the large amount of electricity needed for large-scale cultivation, old or damaged wiring is prone to melt and short. Some black market growers steal power to hide electricity use, and many do not ensure that their wiring is safe. Many growers adapt light cycles so that the lights are on when they are home and off when they are away.
Another fire hazard is plants making contact with hot HID bulbs. Growers using fluorescent bulbs with reasonable air circulation do not have this problem. Word of mouth can be as much a threat to growers as any of the above issues. Often, a few sentences of conversation overheard can result in a tip-off and thus speedy detection. It is for this reason that many growers are reluctant to talk about their cultivation.
Harvesting, drying and curing
[edit]
There may be different goals when harvesting a plant:
- Seeds are harvested when fully developed and often after the accompanying buds have begun to deteriorate.
- Hemp grown for fiber is harvested before flowering,
- Cannabis grown for cloning is not allowed to flower at all.
- Cannabis grown for smoking
A typical indicator that a plant is ready to be harvested for smoking, is when 50% to 70% of trichomes have turned cloudy and 70% of the pistils have turned a reddish brown/amber.[36]
In general, harvesting consists of drying and curing. Curing is an oxidization and polymerization process which takes place in sealed containers of cannabis, over time.
- Dry: Buds placed in a controlled atmosphere for removing moisture content
- Cure: Buds stored in sealed container and left in dark place
Ripeness is defined as the point where THC and other cannabinoid production has reached maximum levels, but before cannabinoids have begun to degrade/breakdown. This is seen under a 30–60x microscope by examining the trichomes on the flowers. When trichomes are undeveloped they are completely clear. They turn white/cloudy which is when trichomes have max levels of cannabinoids. Eventually trichomes start turning amber/purple/red, which is when cannabinoid content has started to degrade. Harvesting before most trichomes have turned white may reduce the overall potency and efficacy time. Harvesting too late (past 90% amber) produces more of a sleepiness effect as the THC degrades to CBN.
Some growers use a brix to measure "sugar" content.
Drying
[edit]
The plants are dried at room temperature in a dark space. It is actually optimal to keep the temperature between 60 and 70 °F (16 and 21 °C) because many terpenoids (molecules that are partially responsible for the psychoactive effects but also largely responsible for the odor of the plant) evaporate at temperatures beyond 70 °F (21 °C). This process can take from a few days to two weeks, depending on the size and density of the buds and the relative humidity of the air. Humidity should be kept between 45% and 55% humidity. Higher humidity will create a mold and mildew risk, while lower humidity will cause the material to dry too quickly. If the plant material dries too quickly, some of the chlorophyll will fail to be converted to a different chemical form which will result in a sub-optimal taste and a harsher smoke when combusted and inhaled. Stable temperature preserves cannabinoids well. Some believe flowers should be hung by their stalks, allowing the internal fluids of the plant to remain in the flowers. Others believe the cut stem is simply a handy non-sticky place from which to hang the plant. Roots are removed, and when the stems in the middle of the largest buds can be snapped easily, the plant is considered dry enough to be cured. Drying is done in a dark place, as THC resins deteriorate if exposed to light and the degradation product CBN forms, significantly altering the cannabinoid profile of the dried flowers.

Cannabis is fully dry for "curing" when the moisture level reaches 55–65% RH. A simple way to check this is by closing the cannabis up in an airtight glass container with a hygrometer. The container is stored for 12 hours at 22 °C (72 °F) and the hygrometer checked. 65% and above readings mean the jar needs to be opened for a few hours and then closed up, to allow more moisture to escape. The jar is again checked after 12 hours and the process repeated until a steady 55% is reached.
Curing
[edit]Once cannabis is dried to 62%, it is sealed in airtight containers to 'cure.' Some growers cure as long as six months, while others do not cure for a week or two, or not at all. As with tobacco, curing can make the cannabis more pleasant to smoke. For the same reasons as when drying, curing jars are stored in a cool, dark place.[37]
Brick weed
[edit]Brick weed is a curing and packaging method of cannabis cultivation that consists in drying the bud for a short period, if at all, and pressing it with a hydraulic press, compacting the whole plant (bud, stems and seeds) into a brick, hence the name brick weed. This method is mainly used in the top cannabis producing countries like Mexico and Paraguay where it is largely exported. Brick weed has a low THC level and less potent aroma and taste.
Pest management
[edit]Both indoor and outdoor growers are likely to confront issues regarding pests. Indoor growers have problems with pests, they can be brought in by hitchhiking on humans or through open exposure to the outdoors. The most common insect pest found on above ground plant parts, leaves, flowers and stems, include aphids, thrips, mites and loopers.[38] Below ground pests that feed on the roots can also be present and include fungus gnats and root aphids.[38] Root aphids, namely the rice root aphid can be particularly problematic due to its high reproductive rate and discrete habitat.
Several plant diseases may also be prevalent and including bud rot, powdery mildew and root rot.[38]
If any of these pests are caught too late, eradication of many destructive species may prove futile unless all infected plants are removed from the space and sterilization methods employed.
Organic and inorganic pest controls
[edit]In any case (indoor or outdoor), experienced growers recommend caution when using chemical pesticides, for they may have toxic effects on the environment, the plants themselves and in turn cannabis consumers. As a general rule, experts mandate the deployment of pesticides clearly marked as "safe to use on food crops." However, the EPA has not registered any pesticides for use on cannabis, making the use of any pesticide on cannabis federally illegal.[39]
Substances that have been used and considered to induce little or no harm include:
- Pyrethrins: Organic and very effective, although sometimes hard to find. Often expensive because of high production cost.
- Azadirachtin: Meets most criteria to be classified as natural insecticide. Biodegradable, non-toxic to mammals. Usually cheaper and easier to find than pyrethrins.
Substances used on cannabis but unknown if harm could occur:
Plant training
[edit]
The modification of a plant's growth habit is called training. Indoor cultivators employ many training techniques to encourage shorter plants and denser canopy growth. For example, unless the crop is too large to be extensively pruned, cultivators remove adventitious growth shoots, often called suckers, that are near the bottom of the plant and/or receive little light and will produce poor quality buds. Some cultivators employ plant training techniques to increase yields indoors:
Topping
[edit]Topping is the removal of the top of the apical meristem (dominant central stem), called the apex or terminal bud, to transfer apical dominance (the tendency for the apex to grow more rapidly than the rest of the plant) to the shoots emanating from the two nodes immediately beneath the pruning cut. This process can be repeated on one or both of the two new meristems, when they become apically dominant, with the same results. This process can actually be repeated nigh infinitely, but over-diffusion of apical dominance produces smaller, lower quality buds, so it is usually done no more than a few times. Topping also causes more rapid growth of all of the branches below the cut while the plant heals.
Pinching
[edit]
Pinching (also called "FIMing") is similar to topping in that it causes lower branches to grow more rapidly, but the apical meristem maintain apical dominance, which is especially useful if the plant has already been topped. Pinching is performed by firmly pinching the apical meristem(s) so as to substantially damage vascular and structural cells but without totally breaking the stem. This causes lower limbs to grow more rapidly while the pinched tissue heals, after which time the stem resumes apical dominance.
Hydroponics
[edit]This section needs additional citations for verification. (November 2021) |

Hydroponic cultivation generally occurs in greenhouses or indoors, although there is no practical obstacle to growing outdoors. In general, it consists of a non-soil medium exposed to a nutrient and water flow.

There are many types of hydroponic systems. If the nutrient solution floods the loose growing medium and recedes for aeration, this is an ebb and flow or flood and drain system. Systems that gradually drip solution onto the medium are drip systems. Systems that intermittently spray roots floating in air are called aeroponic systems. If aerated water runs down a channel lined with a film of rooting medium, this is a nutrient film technique system. A series of tubes intermittently running high flow nutrient solution into the tops of growing containers use a top feed system. Aquaponics, another growing method that is gaining popularity, employs the use of fish water and recirculates that water from the fish holding tank to the growing bed.
Hydroponic systems greatly increase aeration of plant roots, and increase control of nutrient uptake. Hydroponic systems are decidedly more difficult to operate for the amateur or hobby grower, as over-fertilization is common, because there is no soil to act as a nutrient buffer. For this reason, many growers now use coconut fiber as a soil-less medium because of its high drainage and buffering capabilities that make it almost impossible to over-fertilize. Additionally, if a hydroponic system fails, the crop has a high probability of dying as the roots rapidly dry out (this is especially true of aeroponic systems).
There is now a new breed of hydroponic configurations such as the Omega Garden, the B-Pod and the EcoSystem Vertical Growing System that use circular designs to maximize efficiency. This consists of plants being placed or, in the case of the Omega Garden, revolving around a central light that makes maximum use of the light output.
Genetics
[edit]Selection of mother plants
[edit]An important factor while cultivating photoperiod independent (non-autoflowering) cannabis is selecting the best genetics for one's crop. This is frequently done by selecting one or more known strains, or strains with preferred genetics, and then growing a number plants to find which exhibit the characteristics most desirable. These genetics should typically yield at least 1 gram (1/28 oz) per watt per month of flower.

Plant characteristics generally selected for include:
- Overall yield
- Time to fruition
- Resistance to pests
- Geometric traits (uniformity, compactness, flower density, etc.)
- Color
- Flavor and/or aroma
- Appeal to end buyer (known as "bag appeal")
- Psychoactive qualities
- Trichome density and type (stalked or sessile)
Autoflowering strains
[edit]Autoflowering cannabis strains, also known as day-neutral cannabis are a relatively new development for the home cultivator. These autoflower strains are usually crosses that contain high percentages of well known photoperiod strains and Cannabis ruderalis with its autoflowering characteristics. The plant produced from an autoflowering seed will transition from a very short vegetative period, usually 2 to 3 weeks from germination, into flowering regardless of photoperiod. The result is that no separate vegetative and flowering lighting environment is needed. Flowering is dependent on the plant's age, as opposed to time of year or ratio of light and darkness. Autoflowering varieties will bloom from seed in 12/12, 18/6, 20/4 or even 24/0 lighting, referring to light/dark time respectively.
The first autoflowering cannabis seed on the market was the Lowryder #1. This was a hybrid between a cannabis ruderalis called William's Wonder and a Northern Lights #2. The genetics of the ruderalis was still highly present which made for a very low yield and little psychoactive effect.
After many years of autoflower inbreeding seed makers have now come up with autoflowering strains that can produce yields close to the regular strains and still are flowering independent from the light cycle. The first autoflowering strains came from breeder STICH but now almost all major seed companies have their strains of super autoflowers that can grow up to 2 meters (6') and produce yields up to 900 g/m2 (3 oz per sq. ft.) of growth.
Feminized seeds
[edit]Instability of gender is a desirable trait in the wild, where reproduction is the most urgent goal. In cultivation, gender predictability is more helpful, because female plants that have not been pollinated are the most productive of the psychotropic material. It is possible to use a combination of cloning and "shocking" of plants to get them to produce feminized seeds that reliably produce female offspring. A clone retains the same sex throughout its life, so the clone of a female plant is also female.
Environmental stresses sometimes create pollen bearing male flowers on female plants—known as hermaphroditism, "herming", or "hermies".
A method used by organic growers and promulgated by the cannabis breeder Soma, is called 'Rodelization', or letting un-pollinated female plants live several weeks longer than the normal harvest time. In such plants a hermaphroditic trait self-expresses in an effort to continue the genetic line.
Some vendors of feminized seeds assert that hermaphroditic "parents" do not create reliable feminized seeds, since the offspring may retain the tendency of hermaphroditism. Others believe that this method utilizing auto-hermaphroditic traits is offset by grower observations that the tendency to auto-switch sex is no greater in plants grown from seeds made this way, than occurs naturally.
Colloidal silver (commonly abbreviated CS) suppresses ethylene production in bud sites, stimulating male characteristics. Spraying selected leaves, branches and – in cases where a large amount of seed is desired – whole plants with colloidal silver solution has become a preferred method of obtaining feminized seeds. Most plants treated with CS will turn intersex within 2 weeks of triweekly treatment, producing viable pollen within 4. Gibberellic acid has also been used for the same purpose, but it is harder to acquire than colloidal silver and can be difficult to dissolve into solution. One method of obtaining colloidal silver utilizes a small direct current power supply and two pieces of solid silver jeweler's wire, or silver coin.[citation needed]
Some cultivators claim that the genes responsible for hermaphroditism are present and may be expressed under stress from any of the above methods and that once expressed, this characteristic passes to seeds regardless of what activated it. This view, in large part, is incorrect, as a random half of the genes present in each of the parental plants passes to the next generation, regardless of whether the genes that contribute to hermaphroditism were induced by stressors or not. This widely accepted Mendelian model of inheritance (Mendelian inheritance) does allow for genetic mutations that have occurred in the germline of an organism to be passed on to any offspring, but this process applies to all DNA sequences, not just those contributing to hermaphroditism. The inheritance of acquired characteristics (lamarckism) that are not directly coded in the DNA sequence (epigenetics) has recently received much attention in the area of genetic research and could possibly explain any anecdotal evidence for increased hermaphroditism in the offspring of plants induced to a hermaphroditic state. However, a more likely explanation is that by propagating plants easily induced to hermaphroditism by environmental stressors, the frequency of genetic elements contributing to this trait is increased by artificial selection following traditional genetic models of inheritance. Some theories suggest it is possible to selectively breed hermaphroditic cannabis to express the female flowering before the male flowering occurs, though this kind of selective breeding is beyond the capabilities of most cultivators.
Hybrid vigor
[edit]When crossing two strains of cannabis (or two of any plant), the resultant hybrid may possess what is called hybrid vigor. In general, this produces a plant that is healthier, stronger, or quicker growing than its predecessors. Sometimes, in the case of a plant that has been brought back from fruiting (fruition, as mentioned above), it may be beneficial to cross it back with another (close) relative, in the hopes that it becomes invigorated.
Caution should be exercised, as one does not always attain a beneficial cross with hybridizing.
Vegetative propagation (cloning)
[edit]
Like most plants, cannabis has the potential for vegetative propagation, with the most common and simple method being cutting. Cutting is characterized as a cloning method, since the derived plants have identical DNA to the "mother plants".
Under appropriate environmental conditions, a cut part of the cannabis plant, typically from the main stem or a lateral branch, has the ability to produce roots and develop into a whole new plant (the clone), genetically identical to the mother. In cannabis, the production of roots may take anywhere from 5 to 21 days.
The oldest method of cannabis propagation is water cloning. Used for nearly as long as agriculture has been a part of human development, one simply sticks the cut end of clone (cutting) into a small body of water like a glass or bowl and waits. Water cloning can take longer to show roots, but is a truly natural way to propagate any plant that is able.
Marijuana growers often root clones in peat pellets (compressed peat moss) or in rock wool. Another technique that has become popular for rooting clones is aeroponic cloning.[40]
The main steps of hormonal cannabis cutting are as follows:
- Part of the main stem or lateral branch up to 20 cm (8") long is dissected in a non-vertical manner. The bottom 2/3 of leaves are removed.
- The cut end is brought to contact with rooting hormone, according to instructions, to promote root growth and inhibit fungal infection.
- The cutting is placed in an appropriate initial medium such as common soil, compost, perlite, vermiculite, peat moss, sand, rock wool, oasis foam or a combination of those. The initial medium is kept moist and high humidity is maintained in the surrounding air. Elevated humidity levels slow the transpiration rate (water loss from leaves) and prevent the cutting from drying out. Mold is a common hazard due to the higher humidity and stressed cutting. During this phase, the temperature is kept relatively low (25 °C (77 °F)) and direct light is avoided so that the cutting does not dry out. The production from the slower photosynthesis is put into root production.
- After initial root development is evident (usually within three weeks) the cutting is ready to be transplanted into its final grow medium. The high humidity environment is no longer necessary.
-
"Blueberry" cannabis cuttings waiting to be trimmed and re-cut
-
Trimmed with stem re-cut at 45-degree angle below a node
-
New clones under improvised humidity domes
Waste products
[edit]
Prior to the establishment of the first legal cannabis market in Colorado, United States, growers of the plant in the American State of Washington experimented with the use of cannabis waste for pig food. In early 2013, potent cannabis waste products were mixed into the feed of four pigs during the last four months of their lives, resulting in a weight increase of 20 pounds (9.1 kg) to 30 pounds (14 kg) that was registered before the pigs were sent to slaughter in March 2013. Washington State's draft regulations prescribe that cannabis waste must be "rendered unusable prior to leaving a licensed producer or processor's facility," and adds that mixing it with food waste is acceptable. The European Food Safety Authority reported in 2011 that "no studies concerning tolerance or effects of graded levels of THC in food-producing animals have been found in literature." The agency also noted that "no data are available concerning the likely transfer of THC ... to animal tissues and eggs following repeated administration."[41]
Environmental impact
[edit]Environmental impact of cannabis cultivation includes all the environmental issues which occur as a result of cannabis cultivation.
Cannabis agriculture is a massive industry in its scope and extent, yet its environmental impact is much less researched than comparable agricultural products produced at this scale.[42] Many countries around the world are liberalizing their cannabis policy which will make the industry grow, and as the industry grows, so does the urgency to respond to special considerations in environmental impact for this industry.[42]See also
[edit]References
[edit]- ^ Byrne, Genevieve (March 15, 2023). "Energy and Equity in Cannabis Cultivation" (PDF). Institute for Energy and the Environment, Vermont Law and Graduate School. Retrieved April 27, 2023.
- ^ Weaver, Virginia M. (2023). "Fatal Occupational Asthma in Cannabis Production — Massachusetts, 2022". MMWR. Morbidity and Mortality Weekly Report. 72 (46): 1257–1261. doi:10.15585/mmwr.mm7246a2. ISSN 0149-2195. PMC 10684356. PMID 37971937.
- ^ "A cannabis worker died on the job from an asthma attack. It's the first reported case in US". AP News. 16 November 2023. Retrieved 15 June 2024.
- ^ "FACE Program: Massachusetts Case Report 22MA002 | NIOSH | CDC". www.cdc.gov. 14 November 2023. Retrieved 15 June 2024.
- ^ Suman Chandra; Hemant Lata; Mahmoud A. ElSohly (23 May 2017). Cannabis sativa L. - Botany and Biotechnology. Springer. pp. 54–. ISBN 978-3-319-54564-6.
- ^ Cannabis sativa L. – Encyclopedia of Life Archived 28 August 2009 at the Wayback Machine. Eol.org. Retrieved on 7 August 2011.
- ^ American Journal of Botany (2004). "A chemo taxonomic analysis of cannibalized variation in Cannabis (Cannabaceae)". American Journal of Botany. 91 (6): 966–975. doi:10.3732/ajb.91.6.966. PMID 21653452.
- ^ Small, Ernest (1975). "American law and the species problem in Cannabis: Science and semantics". Bull Narc. 27 (3): 1–20. PMID 1041693.
- ^ Greg Green (2003). The Cannabis Grow Bible: The Definitive Guide to Growing Marijuana for Recreational and Medical Use. Green Candy Press. p. 47. ISBN 978-1-931160-17-9.
- ^ Seed Company, Kannabia (5 October 2017). "Which are the best nutrients for marijuana seeds?". Kannabia Seed Company. Archived from the original on 16 September 2021. Retrieved 22 September 2021.
- ^ "Fertilizers' impact on soil health compared | Soil Science Society of America". www.soils.org. Archived from the original on 18 September 2021. Retrieved 5 April 2023.
- ^ Eichhorn Bilodeau, S; Wu, BS; Rufyikiri, AS; MacPherson, S; Lefsrud, M (2019). "An Update on Plant Photobiology and Implications for Cannabis Production". Frontiers in Plant Science. 10: 296. doi:10.3389/fpls.2019.00296. PMC 6455078. PMID 31001288.
- ^ a b Greg Green (2001). The Cannabis Grow Bible (4th ed.). Green Candy Press. ISBN 1-931160-17-1.
- ^ a b Jorge Cervantes (1993). Marijuana Horticulture (5th ed.). ISBN 1-878823-17-5.
- ^ The Marijuana Grower's Guide (1998) by Mel Frank and Ed Rosenthal, typed by Ben Dawson, revised 1992
- ^ Tomoski, Miroslaw (13 December 2018). "Everything You Need To Know About Grow Lights". Herb. Archived from the original on 22 February 2019. Retrieved 21 February 2019.
- ^ "Gas Lantern Routine - Growing Cannabis User Guide". balancemmc.com. 5 November 2016. Archived from the original on 10 December 2017. Retrieved 1 March 2018.
- ^ Grower, Percy (29 January 2011). "12-1 Lighting Method AKA The Gas Lantern Routine - Save 30-50% on your energy costs!". cannabisni.com. Archived from the original on 19 April 2018. Retrieved 1 March 2018.
- ^ Smoker, Jay (5 September 2011). "How To Use The 12/1 Lighting Method For Efficient Marijuana Cultivation". The Weed Blog. Archived from the original on 10 December 2017. Retrieved 1 March 2018.
- ^ Shaw, Barbara H. (13 September 2017). "Understanding Circadian Rhythms in Plants". maximumyield.com. Archived from the original on 11 December 2017. Retrieved 1 March 2018.
- ^ De Meijer, E. P.; Bagatta, M.; Carboni, A.; Crucitti, P.; Moliterni, V. M.; Ranalli, P.; Mandolino, G. (2003). "The inheritance of chemical phenotype in Cannabis sativa L". Genetics. 163 (1): 335–346. doi:10.1093/genetics/163.1.335. PMC 1462421. PMID 12586720.
- ^ "Cannabis Growth Stages: A Breakdown Of The Plant Life Cycle". 22 January 2023. Archived from the original on 18 February 2023. Retrieved 18 February 2023.
- ^ "Greg Green" The Cannabis Grow Bible, 4th edition (2001), page 153,154
- ^ a b c "Grow Hack: Control and Monitor Soil Water Content · High Times". hightimes.com. 3 May 2016. Archived from the original on 12 December 2017. Retrieved 1 March 2018.
- ^ "Photorespiration". Archived from the original on 21 February 2018. Retrieved 1 March 2018.
- ^ "Reefertilizer® - Feed Your Soil, Plants, and Mind". 8 January 2017. Retrieved 13 February 2024.
- ^ "Greg Green" The Cannabis Grow Bible, 4th edition (2001), page 164
- ^ "6 Common Cannabis Greenhouse Problems and How to Solve Them". Cannabis Business Times. Archived from the original on 14 November 2020. Retrieved 5 November 2020.
- ^ "Marijuana found growing in trees". abc27.com. 2010. Archived from the original on 14 July 2014.
- ^ Silver, Johanna (24 March 2020). Growing Weed in the Garden: A No-Fuss, Seed-to-Stash Guide to Outdoor Cannabis Cultivation. Abrams. ISBN 978-1-68335-808-4. Archived from the original on 20 April 2023. Retrieved 9 November 2020.
- ^ "Comprehensive Security Solutions for Cannabis Cultivation". Umbrella Security. Retrieved 14 July 2024.
- ^ Resh, Howard (16 January 2013). Hobby Hydroponics. CRC Press. ISBN 978-1-4665-6942-3.
- ^ Caulkins, Jonathan P. (July 2010). "Estimated Cost of Production for Legalized Cannabis" (PDF). RAND Corporation. Archived (PDF) from the original on 7 December 2017. Retrieved 27 June 2018.
- ^ Amelia Gentleman (25 March 2017). "Trafficked and enslaved: the teenagers tending UK cannabis farms". The Guardian. Archived from the original on 21 September 2017. Retrieved 20 September 2017.
- ^ Bridge, Rowan (17 August 2010). "Children work in 'cannabis farms'". BBC News. Archived from the original on 5 December 2019. Retrieved 30 October 2019.
- ^ "Growers Guide To Cannabis Harvest Timing". www.greenbroz.com. Retrieved 6 December 2023.
- ^ Staff, O. C. C. "Harvesting Basics - Oregon Cannabis Connection". Archived from the original on 11 June 2021. Retrieved 11 June 2021.
- ^ a b c Government of BC (2019). "Integrated Pest Management for Commercial Cannabis in BC" (PDF). Integrated Pest Management. Archived (PDF) from the original on 22 July 2020. Retrieved 21 July 2020.
- ^ "Pesticide Use on Marijuana | Pesticide Registration | US EPA". Archived from the original on 18 November 2016. Retrieved 18 November 2016.
- ^ "Growing Tips: Understanding Cannabis Clones". Retrieved 27 March 2024.
- ^ Jonathan Kaminsky (20 May 2013). "Marijuana waste helps turn pot-eating pigs into tasty pork roast". Reuters. Archived from the original on 24 May 2013. Retrieved 26 May 2013.
- ^ a b Butsic, Van; Brenner, Jacob C (1 April 2016). "Cannabis (Cannabis sativa or C. indica) agriculture and the environment: a systematic, spatially-explicit survey and potential impacts". Environmental Research Letters. 11 (4) 044023. Bibcode:2016ERL....11d4023B. doi:10.1088/1748-9326/11/4/044023.
Further reading
[edit]- Miriello, Ross (2016). Cannabis Botany and Marijuana Horticulture Naturally Medicinal. Maison Hydroponique. ISBN 978-1-5485-0159-4.
- Cervantes, Jorge (2001). Indoor Marijuana Horticulture. Van Patten Publishing. ISBN 1-878823-29-9.
- Cervantes, Jorge (2006). Marijuana Horticulture: The Indoor/outdoor Medical Grower's Bible. Van Patten Publishing. ISBN 1-878823-23-X.
- Clarke, Robert Connell (1981). Marijuana Botany. Berkeley: Ronin Publishing. ISBN 0-914171-78-X.
- Green, Greg (2003). The Cannabis Grow Bible. San Francisco: Green Candy Press. ISBN 1-931160-17-1.
- Herer, Jack (1998). The Emperor Wears No Clothes. Ah Ha Publishing. ISBN 1-878125-02-8.
- Starks, Michael (1990). Marijuana Chemistry: Genetics, Processing & Potency. Ronin Publishing. ISBN 0-914171-39-9.
External links
[edit]Cannabis cultivation
View on GrokipediaPlant Biology
Botanical Characteristics
Cannabis sativa L. is an annual, herbaceous, dioecious plant in the Cannabaceae family, though monoecious forms occur naturally or via breeding.[7][8] Plants grow erect, with heights typically ranging from 0.2 to 5 meters, extendable to 12 meters in cultivation, influenced by genetics and environment.[7][9] Stems are hollow, furrowed, angular, and often branched, featuring woody interiors and internodes up to several decimeters long.[7] The root system comprises a primary taproot extending up to 2.5 meters deep, accompanied by lateral branches.[7] Leaves are palmately compound, with 3 to 13 lanceolate leaflets per leaf, each 3 to 18 cm long and featuring serrated margins and prominent veins; they arrange oppositely at the stem base and alternately above, on petioles 2 to 8 cm long.[7][9] Leaflets measure 0.3 to 3 cm wide, narrowing to linear forms in some varieties.[9] Flowers are imperfect and wind-pollinated, with males forming drooping panicles of small, greenish-white blooms featuring five sepals and prominent stamens, and females clustering in sticky, resinous racemes or spikes enveloped by bracts rich in glandular trichomes.[7][8] Female inflorescences exhibit higher trichome density, contributing to fragrance and stickiness.[7] Fruits are ovoid achenes, 2 to 5 mm long, each enclosing a single seed; cultivated forms yield larger, persistent seeds compared to wild types that shatter easily.[7][9] Morphological traits vary by variety: fiber-oriented hemp types grow tall with minimal branching, while drug-type cultivars branch extensively to optimize female flower production.[7] Subspecies distinctions include taller, sparsely branched C. sativa subsp. sativa (5–18 feet) versus shorter, compactly branched, broad-leaved C. sativa subsp. indica (2–4 feet).[10]Cannabinoid and Terpene Biosynthesis
Cannabinoid biosynthesis in Cannabis sativa occurs predominantly within the glandular trichomes of female inflorescences, where specialized metabolic pathways produce acidic precursors that serve as phytoprotectants. The process initiates in the plastidial compartment with the polyketide pathway, where hexanoyl-CoA condenses with three molecules of malonyl-CoA via the action of tetraketide synthase (TKS), forming a linear tetraketide intermediate, 3,5,7-trioxododecaneoyl-CoA. This intermediate is then cyclized and aromatized by olivetolic acid cyclase (OAC) to yield olivetolic acid (OLA), the polyketide backbone of all major cannabinoids.[11] Subsequently, OLA undergoes prenylation in the cytosol, where aromatic prenyltransferase (such as CsPT1) catalyzes the attachment of geranyl pyrophosphate (GPP), derived from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) via the methylerythritol phosphate (MEP) pathway, to form cannabigerolic acid (CBGA), the central branch-point precursor. CBGA is then diversified by short-chain dehydrogenase/reductase enzymes: tetrahydrocannabinolic acid synthase (THCAS, a 545-amino-acid enzyme encoded by a 1635-nucleotide gene) oxidatively cyclizes CBGA to tetrahydrocannabinolic acid (THCA); cannabidiolic acid synthase (CBDAS) rearranges it to cannabidiolic acid (CBDA); and cannabichromenic acid synthase (CBCAS) yields cannabichromenic acid (CBCA). These acidic forms accumulate in trichome resin, with decarboxylation to neutral cannabinoids (THC, CBD, CBC) occurring non-enzymatically upon heating or prolonged storage.[11][11] Terpene biosynthesis in C. sativa shares precursor pools with cannabinoids, relying on the MEP pathway in plastids for monoterpenes (via GPP) and the mevalonate (MVA) pathway in the cytosol for sesquiterpenes (via farnesyl pyrophosphate, FPP), both generating IPP and DMAPP units that condense into prenyl diphosphates. Terpene synthases (TPS), part of a gene family with 33 unique CsTPS loci identified across cultivars and 19 complete models in the Purple Kush genome, catalyze the committed steps, producing volatile compounds that contribute to aroma, pest deterrence, and potential entourage effects with cannabinoids. Monoterpene synthases (TPS-b subfamily) yield compounds like β-myrcene (via CsTPS3FN), (-)-limonene (CsTPS1FN), (+)-α-pinene (CsTPS2FN), and (E)-β-ocimene (CsTPS6FN), while sesquiterpene synthases (TPS-a subfamily) produce β-caryophyllene, α-humulene, and germacrene B (e.g., via CsTPS9FN). These terpenes accumulate in the same glandular trichomes as cannabinoids, with profiles varying by chemotype, genetics, and environment; cannabis-specific TPS clades drive this diversity.[12][13][12] The co-localization of cannabinoid and terpene production in trichomes underscores shared regulatory mechanisms, such as upregulated gene expression during flowering, influenced by factors like light intensity and developmental stage, which enhance overall secondary metabolite yields. Genetic analyses reveal that TPS gene expression and copy number variations account for chemotype-specific terpene ratios, with high-expression TPS contributing up to the majority of resin volatiles in cultivars like 'Finola'.[12][13]Historical Context
Pre-Modern and Traditional Practices
Cannabis cultivation originated in Central Asia and China, with archaeological evidence indicating human use and likely domestication dating back approximately 5,000 to 6,000 years for fiber production from hemp varieties.[14] Early Neolithic communities along China's Wei and Yellow Rivers integrated cannabis with millet farming, cultivating it primarily for its strong bast fibers used in textiles, ropes, and early paper-making, as evidenced by fabric imprints on pottery from around 4000 BCE.[15] These practices involved open-field sowing of seeds in fertile riverine soils, relying on natural rainfall and seasonal cycles to produce tall, fibrous stalks harvested by hand-cutting and subsequently retted in water to separate fibers from woody cores.[16] By the late Neolithic period, cannabis spread to ritual and medicinal applications, with the earliest written record in China's Shennong Bencaojing (ca. 2700 BCE) attributing Emperor Shen Nung's pharmacopeia to its use for pain relief, inflammation, and as an anesthetic, implying selective cultivation of varieties with higher resin content.[17] In Central Asia, nomadic groups like the Scythians inhaled cannabis vapors in enclosed tents during funerary rites, as described by Herodotus around 440 BCE; residue analysis from wooden braziers in the Jirzankal Cemetery (ca. 500 BCE) confirms high-THC cannabis was burned, suggesting intentional harvesting or cultivation of psychoactive plants on the Pamir Plateau.[2][18] In the Indian subcontinent, traditional cultivation focused on ganja (flowering tops) and bhang (leaves and seeds), rooted in Vedic texts from around 1500–1000 BCE, where plants were grown in Himalayan foothills and Gangetic plains for religious rituals in Shaivite and Hindu practices, often in small plots intercropped with other herbs to enhance spiritual intoxication.[19] These methods emphasized landrace strains adapted to local climates, with male plants culled to promote female flower development, and harvests dried naturally under shade. By the medieval period, cultivation extended to Europe via trade routes, where hemp was farmed densely in fields for naval ropes and sails, as in ancient Greece and Rome, using similar broadcast seeding and retting techniques documented in agronomic texts like Columella's De Re Rustica (1st century CE).[20]Clandestine Cultivation During Prohibition (20th Century)
Following the passage of the Marihuana Tax Act on October 1, 1937, which effectively prohibited non-medical cannabis cultivation in the United States, growers shifted to clandestine operations to evade enforcement.[21] Initial efforts were small-scale, primarily conducted by Mexican laborers in rural areas such as boxcar camps near railroads in the Midwest and West, where plants were grown in hidden patches for personal or local use.[21] These operations remained limited in scope during the 1930s and 1940s, often involving feral or escaped plants from earlier hemp cultivation, with reports of harvesting in places like Chicago and El Paso.[21] The post-World War II era saw a gradual increase in illicit cultivation tied to the counterculture movement of the 1960s, with small backyard plots emerging in urban and suburban settings.[21] By the 1970s, as demand rose and imports from Mexico declined due to eradication efforts like Operation Intercept in 1969, domestic production expanded through "guerrilla growing"—outdoor cultivation in remote, public lands such as national forests and California's Emerald Triangle (Humboldt, Mendocino, and Trinity counties).[22] [23] These methods emphasized stealth, with plants scattered in forested clearings, irrigated via hidden lines, and harvested quickly to minimize detection; the term "guerrilla growing" originated in this decade.[24] Large-scale operations proliferated in the 1980s, particularly in northern California, where the annual illicit crop was valued at $300-400 million, prompting aggressive eradication by the Campaign Against Marijuana Planting (CAMP), which seized 758,526 plants between 1983 and 1988.[21] Similar efforts in Kentucky eradicated over 3 million plants from 1982 to 1988, highlighting the shift to extensive outdoor plots on federal lands.[21] Concurrently, intensified law enforcement drove innovation in indoor cultivation techniques starting in the early 1970s, using high-intensity discharge (HID) lights in concealed spaces like closets or basements to control growth cycles and avoid aerial surveillance.[25] Pioneering guides, such as Mel Frank's Marijuana Grower's Guide published in 1973, detailed these methods, enabling year-round production with higher yields and potency through selective breeding of imported landraces.[22] By the late 1980s and 1990s, indoor growing became predominant in urban areas for its discretion, incorporating early hydroponic systems and ventilation to manage odors and heat signatures, though it required significant electricity and posed risks of fire or detection via utility spikes.[21] Clandestine breeding during this period focused on developing sinsemilla (seedless) females for increased resin production, often from smuggled seeds of Afghan and Thai varieties, laying the groundwork for modern genetics despite the informal, underground nature of the work.[26] These adaptations reflected growers' responses to prohibition's pressures, prioritizing yield, quality, and evasion over open agricultural practices.[21]Post-Legalization Commercial Expansion (2010s–Present)
The commercialization of cannabis cultivation accelerated following recreational legalization in Colorado and Washington in November 2012, marking the first U.S. states to permit regulated adult-use production and sales.[27] This shift transitioned cultivation from predominantly clandestine operations to licensed facilities employing advanced agricultural techniques, with initial investments focusing on secure indoor grows to comply with stringent security and traceability regulations. By 2018, Canada federalized recreational cannabis under the Cannabis Act, effective October 17, enabling nationwide commercial expansion and integrating cultivation into formal supply chains.[28] Licensed cultivation capacity surged, particularly in the U.S., where by 2024, over 4,900 cultivators held licenses in California alone, a fraction of pre-legalization unregulated growers but representative of consolidated commercial operations.[29] The global legal cannabis cultivation market reached an estimated USD 392 billion in 2022, driven by demand for consistent, high-potency products and expanding into medical and recreational segments across 24 U.S. states with adult-use laws by 2024.[30] Innovations in vertical farming, LED lighting, and automated environmental controls reduced production costs and increased yields, with U.S. vertical cannabis farms projected to grow over 30% annually through 2028.[31] These advancements, including hydroponic systems and precision nutrient delivery, addressed scalability challenges in controlled environments, though outdoor cultivation persisted in favorable climates like California's Emerald Triangle for cost efficiency. Economic impacts included the creation of over 440,000 full-time jobs in the U.S. cannabis industry by 2024, spanning cultivation, processing, and ancillary services, contributing approximately $115 billion to the national economy that year.[32] State tax revenues from legal sales exceeded $25 billion cumulatively since 2014, with 2024 alone generating over $4.4 billion, funding public programs while highlighting regulatory burdens that sustained parallel illicit markets due to high excise taxes—often 30-40%—and compliance costs.[33] Industry consolidation favored large operators like Curaleaf, which reported $338 million in Q1 2024 revenue, amid price pressures from oversupply; wholesale flower prices in mature markets like Oregon fell below $1,000 per pound by 2023, reflecting efficiencies but also commoditization risks.[34] Despite growth, challenges persisted, including federal illegality in the U.S. limiting interstate commerce and banking, alongside environmental concerns from energy-intensive indoor operations—U.S. cannabis cultivation consumed electricity equivalent to 1 million households annually by 2012 estimates, though LED adoption mitigated this post-2010.[35] Genetic breeding programs emphasized high-THC and CBD strains, with commercial cultivators prioritizing feminized seeds and clones for uniformity, while tissue culture propagated elite genetics at scale. As of 2025, ongoing expansions in Europe and additional U.S. states signal continued maturation, tempered by market saturation and calls for federal reform to unlock further efficiencies.[36]Fundamental Requirements
Growth Media Options
Growth media, also known as substrates, serve as the physical anchor for cannabis roots while facilitating water retention, nutrient availability, and oxygen exchange essential for root respiration and overall plant vigor. Optimal media balance these properties to prevent issues like root rot from excess moisture or stunted growth from poor aeration, with cannabis Cannabis sativa L. demonstrating adaptability across media types but showing variations in biomass yield, cannabinoid profiles, and susceptibility to pathogens depending on substrate composition.[37] Selection influences cultivation efficiency, as inert media enable precise nutrient dosing via fertigation, whereas organic-rich substrates rely on microbial decomposition for bioavailability.[38] Soil-based media, typically comprising topsoil, compost, and amendments like perlite for drainage, mimic natural terrestrial environments and support beneficial microbial communities that enhance nutrient cycling through mycorrhizal associations and nitrogen fixation. These media retain higher water and cation exchange capacity, buffering pH fluctuations between 6.0 and 7.0, which suits novice cultivators but risks harboring soil-borne pests such as Pythium species or nematodes if not sterilized. Studies indicate soil-grown cannabis achieves comparable inflorescence yields to soilless alternatives under controlled conditions, though growth rates may lag due to slower diffusion of ions compared to solution-based delivery.[39] Advantages include organic flavor profiles attributed to terpene modulation by soil microbes, but disadvantages encompass inconsistent nutrient release leading to deficiencies in mobile elements like potassium during flowering.[40] Soilless organic media, such as coconut coir, peat moss, or blends with vermiculite and perlite, provide a sterile alternative with enhanced drainage and aeration, reducing overwatering risks while maintaining moderate water-holding capacity around 50-70% by volume. Coconut coir, derived from coconut husks, exhibits low electrical conductivity and neutral pH (5.5-6.5), necessitating calcium and magnesium supplementation to counter binding effects, yet it supports root lengths up to 20% longer than peat-based mixes in pot trials due to superior porosity.[37] These substrates yield higher biomass in fiber-type C. sativa genotypes, with cannabinoid contents like CBD varying by up to 15% across coir versus peat, reflecting differences in root zone oxygenation and microbial exclusion.[41] Drawbacks include higher initial costs and potential salt buildup in reused coir, requiring flushing protocols.[42] Inert hydroponic media, including rockwool cubes, expanded clay aggregates (hydroton), and phenolic foam, function primarily as root supports in nutrient film technique (NFT) or deep water culture (DWC) systems, where dissolved fertilizers supply all nutrition via recirculating solutions at EC levels of 1.5-2.5 mS/cm. Rockwool excels in propagation, achieving 80% rooting success versus 13% in organic media for cuttings, attributed to its fibrous structure promoting rapid adventitious root formation.[43] These media enable 20-30% faster vegetative growth and elevated THC concentrations through optimized oxygen levels (up to 25% air-filled porosity), but demand vigilant pH monitoring (5.5-6.2) and system sterilization to avert anaerobic pathogens.[44] Yield advantages are evident in commercial settings, with hydroponic setups reporting 1.5-2 times the dry flower per square meter over soil, though equipment failures can cause total crop loss.[45]| Media Type | Key Components | Advantages | Disadvantages |
|---|---|---|---|
| Soil-based | Topsoil, compost, perlite | Microbial nutrient cycling; pH buffering; organic taste | Pest risks; slow drainage; variable nutrient release[39] |
| Soilless organic | Coco coir, peat, vermiculite | Sterile; high aeration; customizable mixes | Salt accumulation; amendment needs[37] |
| Inert hydroponic | Rockwool, clay pebbles | Precise control; rapid growth; high yields | System dependency; monitoring intensity[44][45] |
Environmental Parameters
Cannabis cultivation demands precise control of environmental parameters to support photosynthesis, transpiration, and metabolic processes, with deviations potentially reducing yield, cannabinoid content, or increasing disease susceptibility. Optimal conditions vary by growth stage—seedling, vegetative, and flowering—and are influenced by factors such as light intensity and CO2 supplementation, as higher resource availability accelerates growth but heightens stress sensitivity. Empirical studies emphasize maintaining these within narrow ranges to maximize biomass and secondary metabolite production, drawing from controlled experiments on Cannabis sativa varieties.[46][47] Temperature profoundly affects enzyme activity and photosynthesis rates; daytime optima range from 24–30 °C across stages, with nighttime drops to 18–22 °C promoting respiration efficiency and preventing heat stress. Exceeding 30 °C impairs photosynthetic efficiency in high-THC strains, leading to reduced CO2 fixation and growth stunting, while below 15 °C halts development. Leaf temperatures in high-light systems target 26–29 °C during vegetative stretch and 27–29 °C in bloom to balance evaporative cooling.[46][47][48] Relative humidity (RH) regulates transpiration and pathogen risk; excessive moisture fosters fungal issues like Botrytis, while low levels cause stomatal closure and nutrient uptake deficits. Vegetative stages tolerate 50–70% RH to support rapid leaf expansion, transitioning to 40–50% in flowering to minimize bud rot without compromising terpene retention. Vapor pressure deficit (VPD), derived from temperature-RH interactions, should span 0.8–1.2 kPa for optimal gas exchange, as deviations disrupt water relations and cannabinoid biosynthesis.[49][50] Light parameters encompass photoperiod, intensity (measured as photosynthetic photon flux density, PPFD), and daily light integral (DLI). Photoperiod strains require 18–24 hours of light for vegetative growth to suppress flowering, shifting to 12 hours to induce bloom via phytochrome-mediated signals. PPFD targets escalate from 100–300 μmol/m²/s in seedlings to 400–600 μmol/m²/s vegetative and 600–1000 μmol/m²/s flowering, with DLI of 20–40 mol/m²/day yielding maximal returns under ambient CO2; photoinhibition occurs above 1000 μmol/m²/s without acclimation. Full-spectrum lighting emphasizing blue (400–500 nm) for vegetative compactness and red (600–700 nm) for flowering is standard.[51][52] CO2 enrichment beyond ambient ~400 ppm enhances photosynthetic rates under saturating light, with 1000–1500 ppm optimal for vegetative and flowering stages to boost yield by 20–30% via increased rubisco activity. Levels above 1500 ppm risk toxicity without proportional temperature adjustments, as elevated CO2 raises canopy heat. Adequate air circulation (0.5–1 m/s velocity) prevents microclimates and strengthens stems, while pH in root zones (5.5–6.5 for hydroponics, 6.0–7.0 soil) indirectly ties to environmental stability by influencing ion availability.[53][54]| Growth Stage | Day Temp (°C) | Night Temp (°C) | RH (%) | PPFD (μmol/m²/s) | Photoperiod (h light/dark) | CO2 (ppm) |
|---|---|---|---|---|---|---|
| Seedling | 20–25 | 18–20 | 60–70 | 100–300 | 18/6 | 400–800 |
| Vegetative | 24–30 | 18–22 | 50–70 | 400–600 | 18/6 | 800–1200 |
| Flowering | 24–28 | 18–22 | 40–50 | 600–1000 | 12/12 | 1200–1500 |
Nutrient Management
Cannabis plants require 17 essential elements for growth, categorized as macronutrients and micronutrients, which must be supplied through fertilizers or media amendments depending on the cultivation system.[56] The primary macronutrients—nitrogen (N), phosphorus (P), and potassium (K)—are needed in largest quantities, with nitrogen supporting vegetative tissue development, phosphorus aiding root and flower formation, and potassium regulating water balance and enzyme activation.[57] Secondary macronutrients include calcium (Ca) for cell wall structure, magnesium (Mg) as a chlorophyll component, and sulfur (S) for protein synthesis. Micronutrients such as iron (Fe), manganese (Mn), zinc (Zn), copper (Cu), boron (B), molybdenum (Mo), and chlorine (Cl) function in enzymatic processes and are required in trace amounts.[58] Nutrient demands vary by developmental stage, with uptake rates increasing overall as plants mature but shifting in composition. In the vegetative stage, optimal hydroponic concentrations are approximately 160–200 mg L⁻¹ N, 30 mg L⁻¹ P, and 60 mg L⁻¹ K, maximizing biomass accumulation through interactions that enhance leaf, stem, and root dry mass.[57] A common NPK ratio for fertilizers during the flowering stage is 1:3:2 (N:P:K), providing low nitrogen to prevent excessive vegetative growth, high phosphorus for bud formation and resin production, and moderate potassium for overall plant health and flower density. Early flowering may use this ratio, transitioning to lower or zero nitrogen (e.g., 0:3:3) in late flowering, with adjustments possible based on factors such as strain, soil, and local conditions.[59] During flowering, requirements adjust to around 194 mg L⁻¹ N and 59 mg L⁻¹ P for peak inflorescence yield (up to 144 g per plant in soilless systems), with K showing no significant yield impact across 60–340 mg L⁻¹ in tested ranges.[56] Shoot concentrations of N, P, and K at the end of the reproductive phase can reach 2.33, 4.90, and 3.32 times those at vegetative maturity, respectively, reflecting heightened demand for reproductive structures.[58] These levels promote nutrient use efficiency but require monitoring to avoid imbalances, as excess P or K can reduce Mg uptake.[57] Solution pH critically influences nutrient solubility and root uptake, with optimal ranges of 6.0–7.0 for soil (shifting to 6.5–7.5 in flowering for better P and K availability) and 5.5–6.5 for hydroponics.[60] Deviations cause lockout, where low pH (<5.5) enhances Fe and Mn solubility but limits P and Ca, while high pH (>7.0) reduces micronutrient availability, leading to deficiencies despite sufficient supply.[60] In soilless systems, regular adjustments maintain availability across the root zone.[56] Deficiencies manifest as stage-specific symptoms: nitrogen shortage causes uniform pale or lime green coloration, especially on older or lower leaves in early flowering—often indicating deficiency or normal reduction during the vegetative-to-flowering transition—progressing to yellowing due to chlorophyll breakdown if uncorrected; magnesium deficiency typically presents as interveinal chlorosis (yellowing between green veins) on older leaves, sometimes with rusty spots or edge yellowing; phosphorus deficiency stunts growth with purple stems and dark green leaves; potassium lack appears as marginal leaf burn and weak stems.[61][62] Micronutrient issues, like Fe deficiency, show interveinal chlorosis in new growth, while toxicities from overapplication—common in high-input grows—result in tip burn and leaf curl, particularly from excess N or salts.[57] Management involves tissue testing, leachate analysis, and balanced formulations, prioritizing synthetic salts for precision in controlled environments over organics, which release nutrients variably. Severe cases of lime green leaves in early flowering may require nutrient adjustments to restore balance.[58]Developmental Stages
Germination and Seedling Phase
Germination of cannabis seeds begins with imbibition, where the seed absorbs water, leading to swelling and enzymatic activation that initiates radicle emergence from the embryo.[63] This process typically requires 3 to 10 days under favorable conditions, with the radicle developing into the primary root followed by the hypocotyl and cotyledons breaking the surface.[64] Optimal temperature ranges from 24°C to 27°C (75°F to 80°F), as temperatures below 21°C slow metabolic processes while exceeding 29°C risks reduced viability.[65] Relative humidity should be maintained at 70% to 90% to prevent desiccation without promoting fungal growth, often achieved using a humidity dome over the medium.[66] Common methods include the paper towel technique and direct sowing into a sterile, light-textured medium like soil, coco coir, or peat-perlite mix with pH 6.0-7.0.[64] As of 2025, no major new techniques have emerged; the paper towel method and direct planting remain the most reliable, achieving 90-100% success rates with consistent warmth, moisture balance, and hygiene using clean tools to prevent rot.[65] For the paper towel method, seeds may be soaked in room-temperature water (pH-neutral, ~18-24°C or 65-75°F) for 12-24 hours to speed initiation. Seeds are then placed between two damp (not soaking) paper towels on a plate, covered to retain humidity, and kept in a dark, warm place at 24-27°C (75-80°F). Daily checks are performed, with transplanting to medium once the taproot reaches 1-2 cm (usually 1-3 days).[65] Direct planting involves pre-moistening the medium (e.g., soil or coco coir with light nutrient solution ~1.0 EC), planting the seed 0.6-1.3 cm (¼-½ inch) deep with taproot down if visible, covering lightly, and keeping the surface moist but not waterlogged. Maintain 24-27°C (75-80°F), initial darkness or low light, and high humidity (65-80%); seedlings emerge in 3-10 days, favored for simplicity and robust root development.[67] Scarification or hydrogen peroxide soaks can enhance germination rates for dormant or aged seeds by breaking seed coats and reducing microbial load, achieving up to 95% success in controlled protocols.[64] Upon radicle growth to 1-2 cm, seedlings are transplanted to avoid root damage, ensuring the crown sits at soil level to minimize rot risk.[68] In the seedling phase, lasting 2-3 weeks post-emergence, plants rely on cotyledon-stored nutrients for initial growth, developing the first true leaves with serrated edges indicative of Cannabis sativa morphology.[69] Light requirements start low at 100-300 µmol/m²/s PPFD on an 18-hour photoperiod to prevent stretching or burn, using full-spectrum LEDs positioned 30-60 cm above.[70] Nutrient application remains minimal, with seedlings in low-fertility media to avoid burn; excess nitrogen can cause leggy growth, while phosphorus supports root establishment at 10-20 ppm in dilute solutions after true leaves appear.[69] Watering should moisten the top 2-3 cm of medium without saturation, allowing the surface to dry slightly between applications to promote oxygenation and deter pathogens.[71] Damping-off, caused by soilborne fungi such as Pythium spp. or Rhizoctonia solani, manifests as stem rot at the soil line, leading to seedling collapse within days of emergence, exacerbated by overwatering, poor drainage, or non-sterile media.[72] Prevention involves using pasteurized substrates, ensuring airflow via fans, and maintaining medium temperatures above 20°C to suppress pathogen activity; chemical fungicides like those containing captan are effective but require adherence to residue limits in legal cultivation.[72] Success rates improve with landrace-specific pretreatments, such as gibberellic acid for dormant varieties, yielding 80-100% germination in tested populations.[68]Vegetative Growth
The vegetative growth stage of Cannabis sativa follows the seedling phase and precedes flowering, during which plants develop extensive root systems, stems, and foliage to support subsequent bud production.[73] This phase typically lasts 2 to 8 weeks, though durations can extend to 16 weeks or more depending on cultivar and grower objectives, allowing for biomass accumulation under non-inductive photoperiods.[73][74] Vegetative growth is promoted by extended light exposure, mimicking long-day conditions that inhibit floral transition in this short-day plant species.[75] Indoors, cultivators maintain an 18-hour light and 6-hour dark cycle (18/6) or continuous 24-hour illumination to sustain vegetative development, as shorter photoperiods trigger flowering.[76][77] Light intensity during this stage should provide a daily light integral (DLI) sufficient for robust leaf expansion, often achieved with high-pressure sodium or LED fixtures delivering 400-1000 µmol/m²/s photosynthetic photon flux density (PPFD).[76] Nutrient demands shift toward nitrogen-rich formulations, with studies indicating optimal uptake ratios where nitrogen constitutes the primary macronutrient to fuel chlorophyll synthesis and protein accumulation.[57] Phosphorus and potassium support root proliferation and structural integrity, respectively, with response surface methodology revealing peak vegetative biomass at N:P:K balances around 200:100:200 mg/L in hydroponic systems.[57] Plant training techniques, applied exclusively in the vegetative phase, manipulate apical dominance to increase lateral branching and canopy uniformity. Low-stress training (LST) involves gently bending stems and securing them horizontally to expose lower bud sites to light, enhancing photosynthetic efficiency without hormonal disruption.[78] High-stress methods like topping—severing the main stem above the fifth node—induce multiple colas but delay growth by 5-7 days per application, allowing recovery within 1-2 weeks.[78] These interventions, performed under 18/6 lighting, can double or triple yield potential by optimizing light distribution, though overuse risks stress-induced hermaphroditism in genetically susceptible strains.[78][79] Grow medium and density influence vegetative morphology; for instance, higher plant densities (up to 36 plants/m²) compact growth and reduce internode length, while extended vegetative periods (up to 4 weeks) allocate more biomass to shoots over roots.[74] Rockwool, peat-perlite, and coconut coir substrates support comparable early growth, with coir favoring sustained water retention for prolonged veg cycles.[80] Stepwise transplanting during this phase promotes denser, healthier root growth by encouraging roots to actively colonize new medium volumes, improves moisture control as smaller pots dry faster to reduce overwatering risk, and minimizes plant stress for faster recovery and overall vegetative expansion.[81][82] Guidelines recommend pots sized approximately 2–5 L per 30 cm of plant height, starting from 1 L after 1–3 weeks once rooted through, progressing to 3–5 L for 2–4 more weeks before final transplant to 18 L prior to flowering.[81][82] Monitoring for deficiencies, such as nitrogen chlorosis manifesting as yellowing lower leaves, enables timely adjustments to maintain exponential growth rates of 1-2 nodes per week.[57]Flowering Initiation and Maturation
Flowering in Cannabis sativa is primarily induced in photoperiod-sensitive varieties by reducing the daily light period to approximately 12 hours of uninterrupted light followed by 12 hours of darkness, mimicking the shortening days of late summer or autumn. This photoperiodic trigger relies on a critical night length exceeding 10 hours, during which phytochrome-mediated signaling pathways promote the transition from vegetative growth to reproductive development, including the formation of inflorescences.[83][75] The optimal light duration for initiation varies by cultivar between 9 and 14 hours, though deviations from the standard 12:12 cycle can delay or alter floral responses, with longer photoperiods potentially sustaining vegetative traits or provoking incomplete initiation.[75] Autoflowering strains, derived from Cannabis ruderalis genetics, bypass this requirement and initiate flowering based on age rather than light cues, typically after 3-4 weeks of vegetative growth.[75] Once initiated, the flowering phase spans 8-16 weeks depending on strain genetics, with indica-dominant varieties often maturing faster (8-10 weeks) than sativa-dominant ones (10-16 weeks). Early flowering involves rapid calyx and pistil development on female plants, where resin glands (trichomes) begin producing cannabinoids and terpenes; male plants form pollen sacs within 2-3 weeks if not culled. Nutrient demands shift toward higher phosphorus (P) and potassium (K) relative to nitrogen (N), with formulations around 200-250 ppm N-P₂O₅-K₂O recommended at bloom onset to support bud site proliferation and energy transfer, while avoiding excess N that promotes leafy growth over floral density. A lime green or pale green appearance of leaves in early flowering is often a normal response to the reduced nitrogen levels in bloom nutrients; however, uniform paling progressing to yellowing on older leaves may indicate nitrogen deficiency, distinguishable from magnesium deficiency which typically presents as interveinal chlorosis on older leaves.[84][61] Magnesium supplementation aids chlorophyll stability and enzyme function, with secondary nutrients like calcium preventing deficiencies that manifest as bud rot or stunted maturation.[85] Mid-flowering (weeks 4-6) sees peak biomass accumulation in buds, requiring consistent micronutrient availability (e.g., boron, iron) to sustain metabolic processes without inducing toxicity from over-fertilization.[86] Environmental control is critical to prevent stress-induced hermaphroditism or yield loss. Daytime temperatures of 20-25°C (68-78°F) and nighttime drops to 18-22°C optimize enzyme activity and terpene retention, while relative humidity should be maintained at 40-50% to minimize mold risks on dense buds, gradually lowering to 30-40% in late stages.[87] Light intensity increases to 800-1500 µmol/m²/s PAR to drive photosynthesis without light stress, with CO₂ enrichment to 1000-1500 ppm enhancing yield under controlled conditions. Irrigation frequency adjusts to soil moisture levels, as flowering roots are less expansive, demanding precise management to avoid waterlogging that impairs oxygen uptake and cannabinoid synthesis.[88] Maturation culminates in harvest readiness, assessed primarily by trichome morphology under magnification: immature clear trichomes indicate under-ripeness, while 70-80% milky (cloudy) with 20-30% amber signals peak THC potency before degradation into CBN, yielding balanced psychoactive effects.[89] Supplementary indicators include pistil senescence—50-70% darkened and curled—and bud density, with over-maturity (excess amber trichomes >30%) correlating to sedative profiles but potential potency loss.[90] Nutrient flushing with plain water for 1-2 weeks pre-harvest removes residuals, improving taste and smoothness, as verified by reduced ash content in combusted samples.[91] Harvest timing directly influences cannabinoid profiles, with empirical data showing 10-20% THC variance based on a 7-14 day delay post-initial ripeness.[92]Cultivation Methods
Outdoor and Greenhouse Systems
Outdoor cannabis cultivation is permitted in many U.S. states where recreational or medical cannabis is legal, including California, Oregon, Washington, Colorado, Michigan, Nevada, Arizona, Maine, Massachusetts, Illinois, Maryland, Minnesota, Missouri, New Jersey, New Mexico, New York, Ohio, Virginia, Vermont, Rhode Island, Connecticut, and Delaware for personal grows, typically allowing 6-12 plants per adult with requirements for security, visibility screening, and setbacks; commercial outdoor cultivation is licensed in states like California, Oregon, and Colorado, subject to strict zoning, security, and environmental regulations. No major federal or widespread state changes are projected for 2025-2026 that would significantly alter outdoor cultivation legality, though local regulations and new state legalizations could evolve. This method leverages natural sunlight, which provides intense, full-spectrum light without energy costs, enabling larger plant sizes and potentially higher yields per plant compared to controlled environments, along with lower operational costs, environmentally sustainable practices with reduced carbon footprint, and superior terpene profiles from natural growth conditions.[93] However, it faces disadvantages such as high dependence on local climate and growing season length—ideal in Mediterranean climates like California and Oregon, but challenging in northern or humid states requiring greenhouses or facing shorter seasons—increased risks of pests, mold, diseases, and wildlife damage, security and theft vulnerabilities due to visibility, and regulatory hurdles like fencing and odor control. Plants thrive in climates with daytime temperatures of 20-25°C during vegetative and early flowering stages, tolerating brief fluctuations but suffering from prolonged extremes like frost or excessive heat above 30°C, which can stress plants and reduce cannabinoid production.[94] Photoperiod varieties are typically started indoors or in greenhouses in mid-spring—around May in northern hemispheres—for transplanting after hardening off, allowing vegetative growth through summer and automatic flowering initiation as daylight shortens to 14-14.5 hours in late summer.[95][96] Site selection prioritizes south-facing locations with at least 6-8 hours of direct sunlight daily, well-drained loamy soil amended to pH 6.0-7.0, and protection from strong winds to minimize physical damage and evaporation.[93] Soil preparation involves testing for nutrients and incorporating organic matter like compost to support root development, as cannabis roots deeply in open ground, potentially reaching 1-2 meters.[97] Watering relies on rainfall supplemented by irrigation during dry spells, with mature plants requiring 5-10 liters per day depending on climate and soil retention.[98] Under optimal conditions in Mediterranean-like regions, individual plants can yield 500 grams to several kilograms of dried bud, far exceeding indoor limits due to unrestricted vertical growth and solar intensity.[99] However, vulnerability to environmental risks—such as heavy rains causing root rot, hail damaging buds, or unseasonal cold halting growth—necessitates strain selection for local resilience, like indica-dominant hybrids for cooler areas.[97] Pests like spider mites or caterpillars proliferate without barriers, demanding integrated pest management including companion planting or organic sprays rather than reliance on isolation.[98] Greenhouse systems bridge outdoor and indoor methods by enclosing plants in translucent structures that capture 70-90% of natural light while shielding from precipitation, wind, and extreme temperatures.[100] Ventilation fans, automated shading cloths, and heating systems maintain vegetative temperatures of 20-30°C and flowering ranges of 18-26°C, with humidity controlled at 40-70% to prevent mold in denser canopies.[101] This setup extends the growing season beyond outdoor limits, enabling two harvests per year in temperate zones or year-round production in milder climates through supplemental LED lighting during short days.[100][102] Initial infrastructure costs—ranging from $50-100 per square meter for basic hoop houses to higher for climate-controlled glasshouses—exceed outdoor setups but yield operational savings via reduced artificial lighting and potential for scaled production of 1-2 kg per square meter annually.[103] Enhanced environmental control mitigates outdoor risks, such as UV degradation or pollinator intrusion via netting, while allowing hydroponic or soilless media for precise nutrient delivery.[104] Empirical comparisons indicate greenhouse-grown cannabis retains higher terpene profiles due to moderated stress, though energy for climate systems contributes to a carbon footprint intermediate between low-impact outdoor and high-energy indoor methods.[105] Drawbacks include higher upfront investment and maintenance for systems like evaporative cooling in humid areas, where poor airflow can foster pathogens like powdery mildew.[106]Indoor Controlled Environments
Indoor controlled environments for cannabis cultivation involve enclosed spaces such as grow tents, rooms, or cabinets where growers manipulate variables like light cycles, temperature, humidity, and CO2 levels to optimize plant growth and yield.[107] This method enables year-round production regardless of external weather, providing precise control that can result in higher-quality buds with consistent cannabinoid profiles compared to outdoor methods.[97] Indoor setups typically yield more per square foot due to intensified resource application, with facilities achieving up to ten times the crop output of traditional farms.[108][109] Lighting systems are central, with high-intensity discharge (HID) lamps like high-pressure sodium (HPS) and metal halide (MH) historically dominant for their high light intensity promoting rapid vegetative growth and flowering.[110] Light-emitting diodes (LEDs) have gained prevalence for their energy efficiency, customizable spectra tailored to cannabis photosynthetic needs, and reduced heat output, potentially increasing yields by 30% over older LEDs while lowering electricity use.[111] Photoperiod strains require 18-24 hours of light daily during vegetative stages and 12 hours during flowering to trigger bud development, often consuming 200-400 watts per square foot.[112] Climate control encompasses maintaining temperatures of 70-88°F (21-31°C) under CO2 supplementation, with relative humidity at 40-70% varying by growth stage to prevent mold and stress.[113] Ventilation systems with exhaust fans, carbon filters, and air circulation prevent hotspots, manage odors—the strong, pungent smell from even a single cannabis plant during flowering, which can become noticeable throughout an entire house or apartment without controls and intensifies in the last 4-6 weeks due to elevated terpene production—and facilitate CO2 enrichment up to 1200-1500 ppm to enhance photosynthesis. Carbon filters or similar measures are commonly recommended even for single-plant setups to contain the odor.[114] Heating, ventilation, and air conditioning (HVAC) often comprise over 50% of energy use, alongside dehumidification to handle transpiration rates.[115] Despite benefits like pest minimization through sealed environments and discretion, indoor cultivation demands significant upfront costs of $75-100 per square foot for commercial setups and high operational energy, up to 3,000 kWh per pound of dried flower, driven by lighting (38%) and climate systems (51%).[116][117] This intensity contributes to environmental footprints exceeding traditional agriculture, though LEDs and efficient HVAC mitigate some demands.[118][119]Hydroponic and Aeroponic Techniques
Hydroponic cultivation of cannabis involves growing plants in nutrient-enriched water solutions without soil, using inert substrates such as rockwool, perlite, or clay pebbles to support roots. This method delivers nutrients directly to the root zone, enabling precise management of pH (typically 5.5-6.5), electrical conductivity, and elemental ratios tailored to cannabis's demands during vegetative and flowering stages.[120] Systems include deep water culture (DWC), where roots are submerged in oxygenated reservoirs; nutrient film technique (NFT), with a thin film of solution flowing over roots; and ebb-and-flow, which periodically floods and drains trays.[121] These setups facilitate faster growth rates and higher yields compared to soil, as roots access oxygen and nutrients without soil buffering delays, with studies reporting up to 687 grams of dry inflorescence per plant in controlled hydroponic environments using screen-of-green training.[122] However, hydroponics demands vigilant monitoring to prevent imbalances, such as nutrient lockout from pH drifts or root zone hypoxia if aeration fails.[123] Aeroponic techniques extend hydroponics by suspending cannabis roots in air within enclosed chambers, periodically misting them with a fine nutrient aerosol via high-pressure nozzles (typically 50-100 psi). This maximizes root exposure to oxygen—up to 10 times more than submerged hydroponic roots—promoting enhanced cellular respiration and nutrient uptake efficiency.[124] Aeroponics can accelerate vegetative growth by 20-30% and boost yields through superior root development, with some cultivators achieving harvests exceeding those of standard hydroponic systems due to reduced transplant shock and optimized resource use.[125] Water consumption is minimized, often 90-98% less than soil methods, as misting recycles solutions with minimal evaporation or runoff.[126] Drawbacks include high initial costs for pumps, timers, and foggers, plus vulnerability to system failures like clogged nozzles or power outages, which can desiccate roots within hours, necessitating backup redundancies.[127] Comparatively, while hydroponics offers scalability and forgiveness for novices through diverse system designs, aeroponics excels in efficiency for high-density operations but requires advanced technical oversight to maintain mist uniformity and prevent pathogen ingress in the open root zone. Both methods outperform soil in controlled indoor settings by mitigating variables like soil-borne diseases, though empirical data on cannabis-specific cannabinoid profiles show minimal differences attributable to medium alone, emphasizing environmental consistency over technique.[128][129]Genetic and Propagation Strategies
Strain Selection and Breeding
Strain selection in cannabis cultivation prioritizes genetic traits aligned with cultivation goals, environmental conditions, and end-use applications, such as high THC for recreational varieties or elevated CBD for medical ones. Key factors include cannabinoid and terpenoid profiles, which determine chemotypes; photoperiod response for flowering control; plant morphology like height and branching for space efficiency; yield per plant or square meter; and resilience to abiotic stresses (e.g., drought, temperature extremes) or biotic threats (e.g., powdery mildew via Mlo-like genes). Landrace accessions, evolved over millennia in regions like Afghanistan (short, resinous indicas) or Thailand (tall sativas), provide baseline diversity but often require hybridization for modern yields, as pure landraces exhibit inconsistent cannabinoid stability under controlled conditions.[130][131] Breeding cannabis traditionally relies on phenotypic selection and controlled crosses, leveraging its dioecious nature to isolate female plants for sinsemilla production while using male pollen for hybridization. Cannabis pollen remains viable for only 2–3 days under normal room temperature conditions after release. With proper storage—dry, dark, airtight, and ideally frozen—viability can extend to 12 months or longer, though moisture and temperature fluctuations significantly reduce shelf life.[132] Mass selection from diverse populations, followed by inbreeding and backcrossing over 5–9 generations, stabilizes traits like uniform flowering (e.g., in "Skunk No. 1"), though clandestine pre-2010s efforts often prioritized THC potency, raising average levels from 4% in 1995 to over 12% by 2014 without preserving genetic records. Open-pollination or polycross methods accelerate diversity but risk instability, necessitating progeny testing for heritability.[133][131] Genomic advancements enable marker-assisted selection (MAS), using single nucleotide polymorphisms (SNPs) or simple sequence repeats (SSRs) to target quantitative trait loci (QTLs) for cannabinoid biosynthesis, such as THCAS/CBDAS gene variants identified in Purple Kush and Finola genomes. Panels of 22–41 markers facilitate early selection for traits like sex determination (via Y-linked loci SuF and M) or pathway-specific SNPs, reducing breeding cycles from years to months. Emerging techniques include tissue culture micropropagation (yielding 2–13.8 shoots per explant via nodal segments) for clonal fidelity and CRISPR/Cas9 editing, which achieved targeted mutations in hemp by 2021 but faces genotype-dependent regeneration rates below 10% in drug-types. Polyploid induction has boosted CBD content by 9% in select lines, yet regulatory barriers and low transformation efficiency limit widespread adoption.[133][130][131] Challenges persist due to drug-type cannabis's narrowed genetic base from inbreeding, complicating introgression of resistance traits without diluting cannabinoid profiles, and recalcitrance to in vitro protocols varying by genotype (e.g., 96.6% success in leaf explants for some hemp but near-zero in high-THC strains). Breeders mitigate this through hybrid vigor in F1 crosses, but empirical validation via gas chromatography-mass spectrometry ensures chemotype fidelity across generations.[131][130]Cloning and Vegetative Propagation
Vegetative propagation of cannabis primarily utilizes stem cuttings from selected mother plants to produce genetically identical clones, bypassing seed germination and ensuring preservation of desirable traits such as cannabinoid profiles and morphology.[134] This method maintains cannabinoid production consistency across generations, as demonstrated in industrial hemp where successive cloning retained phenotypic stability.[135] Mother plants are chosen for vigor, disease resistance, and specific attributes like branching structure, typically after evaluating clones through flowering trials to confirm traits.[136] Cuttings are taken from healthy, vegetative branches, ideally 10-15 cm long with 2-4 nodes, using a sterilized blade for a 45-degree cut to maximize vascular exposure.[4] Lower leaves are removed to reduce transpiration, and the basal end is dipped in rooting hormone containing indole-3-butyric acid (IBA) to stimulate adventitious root formation.[137] Optimal conditions include high humidity (80-90%) via propagation domes, temperatures of 22-25°C, and continuous low-intensity light (18-24 hours photoperiod) to promote rooting within 7-14 days.[138] Rooting success rates range from 70-90% under controlled environments, with factors like retaining three leaves per cutting increasing efficacy by 15% compared to fewer or more.[139][137] Compared to seed propagation, cloning accelerates production by eliminating the 1-2 week germination phase and juvenile growth, yielding uniform plants ready for vegetative expansion or flowering induction without variability in sex or potency.[140] However, reliance on clones risks amplifying pathogens from the mother plant, necessitating rigorous sanitation to prevent systemic spread, and limits genetic diversity, potentially reducing resilience to environmental stresses.[141] Mother plants require perpetual vegetative maintenance under extended photoperiods, demanding space and resources, though this enables scalable operations in commercial cultivation.[142]Autoflowering and Feminized Varieties
Autoflowering cannabis varieties derive their automatic flowering trait from hybridization with Cannabis ruderalis, a subspecies characterized by age-dependent flowering independent of photoperiod changes.[143] These plants typically initiate flowering 2-4 weeks after germination, completing the life cycle in 8-10 weeks from seed to harvest, enabling multiple harvests per year in suitable climates.[144] The ruderalis genetics confer resilience to harsher conditions but often result in smaller stature, with plants averaging 60-100 cm in height, and yields 20-50% lower than photoperiod counterparts due to limited vegetative growth time.[145] [146] Cultivators favor autoflowers for their simplicity, as they require consistent 18-24 hours of light daily without switching schedules, reducing energy costs in indoor setups and minimizing light leak risks outdoors.[147] However, the fixed short lifecycle limits training techniques like topping or SCROG, potentially capping potency, with THC levels in modern hybrids reaching 20-25% but historically lower due to ruderalis dilution.[145] [148] Breeding advancements since the early 2000s have stabilized F1 hybrids, improving vigor and cannabinoid profiles while retaining the autoflowering stability.[149] Feminized varieties are produced by inducing female plants to generate pollen through chemical stressors like silver thiosulfate (STS) or colloidal silver, which inhibit ethylene production and promote male flower development without genetic male presence.[150] This pollen fertilizes female flowers, yielding seeds that germinate as females at rates exceeding 99%, eliminating the need to cull males and maximizing space efficiency in cultivation.[151] Techniques involve applying STS solution (typically 0.1-0.3% silver nitrate with sodium thiosulfate) to selected branches for 10-14 days, followed by pollination after pollen sac maturation in 2-3 weeks.[152] The primary advantage of feminized seeds lies in consistent bud production, as all plants develop sin semilla flowers, boosting yields by avoiding pollination-induced seed formation that reduces resin quality.[153] Drawbacks include heightened hermaphroditism risk under stress, with some lineages showing 5-10% intersex traits, and reduced breeding utility since no true males emerge for traditional crosses.[154] Regular seed banks report feminized lines maintain genetic stability comparable to regulars when sourced from reputable breeders, though overuse in selections can narrow diversity.[155] Many commercial strains combine both traits, offering feminized autoflowers that provide female-only, rapid-cycle plants ideal for novice growers or high-turnover operations, though hybrid vigor varies by breeder.[156] These varieties have proliferated since the 2010s, comprising over 70% of seed market sales in legalized regions due to efficiency gains.[157]Crop Management Techniques
Training and Pruning Methods
Training and pruning methods in cannabis cultivation aim to optimize plant architecture, enhancing light penetration, airflow, and resource allocation to inflorescences, potentially increasing yields and cannabinoid content. These techniques manipulate growth patterns, with low-stress training (LST) focusing on non-invasive bending of stems and branches to create a flat canopy; LST's low-stress nature allows immediate exposure to direct sunlight without needing shaded or dark recovery periods, as plants recover quickly—often in hours or 1-2 days—without significant stem damage, contrasting with high-stress methods that require longer recovery; this enables maximizing direct sunlight in outdoor cultivation by exposing previously shaded parts, which is beneficial for growth. Practitioners train gently to avoid breaking or cracking stems, and if accidental damage occurs, monitor for stress signs like wilted or burned leaves while allowing 1-2 days for recovery, ensuring adequate watering and ventilation if temperatures exceed 30-32°C with low humidity to prevent heat stress, and noting that some prefer performing LST in the late afternoon for overnight adjustment though not required. High-stress training (HST) includes topping—severing the apical meristem to induce multiple colas—and supercropping, which involves pinching stems to promote bushier development. Screen of green (SCROG) extends LST by using a horizontal net to support and evenly distribute branches, maximizing light exposure in limited spaces.[158][159] Pruning encompasses defoliation, removing fan leaves to reduce shading and improve ventilation, and lollipopping, stripping lower foliage and branches to redirect energy upward. These practices are typically applied during vegetative or early flowering stages to minimize stress recovery time. Empirical studies indicate variable impacts: topping increased inflorescence yield by 24.5% and 12.9% in hemp cultivars 'Fedora 23' and 'Futura 75', alongside CBD elevations of 22.7% and 18.1%, respectively.[160] In medicinal cannabis 'Topaz', double pruning raised CBDA by 59% and THCA by 50%, with defoliation boosting several cannabinoid concentrations.[160] However, a controlled trial found topping yielded higher inflorescence biomass (18.5 g per plant versus 16.3 g in controls) but no statistically significant differences in total CBD yield across pruning variants (p=0.0923), underscoring harvest timing's greater influence.[161] While grower reports attribute LST and SCROG to yield gains through better photoperiod utilization, peer-reviewed evidence remains limited, with training often integrated into architecture studies showing improved cannabinoid uniformity rather than quantified yield uplifts. Excessive HST or untimely pruning risks stunted growth or reduced photosynthesis, as leaf removal can transiently lower net carbon assimilation without compensatory bud site development. Optimal application depends on strain genetics, with indicas responding better to HST for compactness and sativas to LST for height control.[160][161][162]Irrigation and Crop Steering
Irrigation strategies in cannabis cultivation incorporate crop steering to direct plants toward vegetative or generative growth by modulating water volume, frequency, electrical conductivity (EC), and dry-back periods, often integrated with vapor pressure deficit (VPD) and lighting for balanced development. Vegetative steering emphasizes higher substrate moisture retention (e.g., allowing 20-30% dry back post-irrigation) and lower EC levels to promote expansive root and shoot growth, maintaining consistent hydration to support biomass accumulation. In contrast, generative steering during flowering shifts to strategies promoting drainage and controlled stress, such as shorter cycles with higher leachate fractions (e.g., 10-20% dry back) and elevated EC to redirect resources toward inflorescence development and cannabinoid synthesis, enhancing root oxygenation while preventing waterlogging. These techniques, prevalent in soilless media and hydroponics, optimize yield and quality from propagation through harvest by responding to plant signals like drain EC and weight trends, though efficacy varies by genetics and environment.[163][164][165]Pest and Pathogen Control
Integrated pest management (IPM) forms the cornerstone of pest and pathogen control in cannabis cultivation, emphasizing prevention, monitoring, and minimal intervention to reduce reliance on synthetic chemicals while protecting plant health and end-product safety.[166] [167] IPM strategies integrate cultural practices, such as sanitation and environmental optimization, with biological agents and targeted treatments, particularly in controlled indoor environments where pests can proliferate rapidly due to stable conditions.[168] Regular scouting using sticky traps, magnification tools, and visual inspections detects infestations early, when populations are below economic thresholds.[169] Common pests include spider mites (Tetranychus urticae), aphids (Aphis spp.), thrips (Frankliniella spp.), and whiteflies (Bemisia tabaci), which can cause leaf stippling, sap extraction, and viral transmission, leading to yield reductions of up to 50% in severe cases.[169] [170] Spider mites, thriving in low-humidity conditions common to indoor grows, are effectively suppressed by releasing predatory mites such as Phytoseiulus persimilis at rates of 2-10 per square meter, which consume 5-20 prey daily under optimal temperatures of 25-30°C.[171] [172] For aphids, biological controls like lacewing larvae (Chrysoperla carnea) or lady beetles (Hippodamia convergens) provide predation, achieving 70-90% control when introduced preventively alongside neem oil applications that disrupt feeding without residues harmful to beneficials.[173] Thrips and whiteflies respond to entomopathogenic fungi like Beauveria bassiana, applied as sprays, which infect and kill nymphs within 3-7 days under high humidity.[170] Cultural measures, including quarantining new plants and maintaining negative pressure ventilation, prevent introductions, as pests often enter via clones or workers.[168] Pathogens such as powdery mildew (Golovinomyces ambrosiae), Botrytis gray mold (Botrytis cinerea), and root rots (Pythium or Fusarium spp.) pose significant threats, exacerbated by dense canopies and fluctuating microclimates.[174] Powdery mildew, identifiable by white fungal mats on leaves, is managed through weekly applications of potassium bicarbonate (e.g., MilStop at 2-3 lbs/100 gallons), which alters leaf pH and inhibits spore germination, reducing incidence by 80% in trials.[174] Botrytis, causing bud rot in high-humidity flowering stages (>90% RH), requires cultural controls like increasing airflow via fans and spacing plants 0.5-1 meter apart to limit free moisture on tissues for over 8 hours, supplemented by biofungicides such as Bacillus subtilis strains.[175] [176] Root pathogens are prevented by sterilizing substrates and treating irrigation water with UV irradiation (dosage >40 mJ/cm²) or chlorine dioxide, which eliminates propagules and cuts infection rates by over 95%.[177] In regulated operations, only OMRI-listed or state-approved products are used to avoid cannabinoid degradation or consumer health risks from residues.[178] Challenges in IPM efficacy arise from variable biological agent performance in cannabis's high-value, low-tolerance systems, where incomplete control can necessitate restarts; thus, combining agents (e.g., predatory mites with humidity-tolerant Neoseiulus californicus for spider mites) enhances resilience.[173] Outdoor cultivations face additional pressures from migratory pests like caterpillars, mitigated by row covers or Bacillus thuringiensis applications targeting larvae.[169] Overall, proactive environmental management—targeting 40-60% RH and temperatures below 28°C—underpins success, as poor conditions amplify outbreaks regardless of interventions.[167]Harvesting and Processing
Timing and Techniques
Harvesting cannabis plants requires precise timing to optimize cannabinoid content, terpene profiles, and yield, as premature or delayed harvest can reduce THC levels by up to 20-30% or shift potency toward sedative CBN.[179] Prematurely harvested buds remain usable but exhibit reduced potency, underdeveloped cannabinoids and terpenes, and harsher smoke due to higher chlorophyll content; careful drying prevents mold, with discard advised only for severely underdeveloped or moldy material.[180][181] Primary indicators include pistil maturation, where 50-70% turn amber, orange, or brown and curl inward, signaling bud swelling and resin production peak, typically 8-10 weeks into flowering for photoperiod strains.[182] Trichome examination via 30-60x magnification jeweler's loupe or microscope provides the most reliable visual cue: harvest when 70-90% appear milky white (indicating maximum THC accumulation) and 10-20% amber (for balanced euphoria without excessive sedation), as clear trichomes denote immaturity and excessive amber signals degradation.[183] Strain genetics influence this window, with indicas often ready earlier than sativas, and autoflowers harvested based on calendar days (70-90 from seed) rather than light cycles.[184] Commercial operations increasingly supplement visual assessment with laboratory testing, such as HPLC analysis of cannabinoid ratios, to confirm harvest readiness and ensure compliance with potency standards, reducing variability from subjective observation.[185] Environmental factors like temperature fluctuations or nutrient deficiencies can accelerate or delay maturation, necessitating daily monitoring in the final two weeks.[186] Techniques prioritize minimizing plant stress and contamination to preserve resin integrity. Plants are cut at the base of the main stem or in sections (bucking branches) using sterilized, sharp pruning shears to prevent microbial introduction and bud damage, often in low-light conditions to avoid photosynthetic shock.[187][188] Wet trimming—removing fan leaves and sugar leaves immediately post-harvest—facilitates even drying by improving airflow but risks terpene volatilization from exposed resin glands; dry trimming post-drying (after 5-10 days at 18-24°C and 45-55% humidity) retains more volatiles yet demands precision to avoid mold.[189] Branch-by-branch harvesting staggers the process, allowing continued ripening on remaining plants while reducing overcrowding risks during initial drying.[190] Yields average 0.5-1 gram per watt of light indoors when timed correctly, with improper techniques like dull tools causing up to 10% resin loss.[191]Drying, Curing, and Storage
Drying cannabis inflorescences post-harvest involves reducing moisture content from approximately 75-80% to 10-15% to inhibit microbial growth and preserve bioactive compounds.[192] Traditional methods include hanging whole plants or branches upside down in a dark, well-ventilated room maintained at 18-24°C and 45-55% relative humidity (RH), with gentle airflow to prevent mold formation; laying buds flat on surfaces like the floor should be avoided as it limits airflow around all sides, potentially causing uneven drying or contamination.[193][194] This process typically lasts 5-10 days, until small stems snap cleanly rather than bend, indicating equilibrium moisture content suitable for further processing.[192] Faster methods, such as convective hot air drying above 40°C or freeze drying, can shorten duration to 24-48 hours but risk terpene volatilization and cannabinoid degradation, with studies showing up to 11% loss in total cannabinoids at elevated temperatures.[195] Controlled atmosphere drying in specialized chambers has demonstrated potential to reduce drying time by at least 60% while maintaining cannabinoid profiles comparable to air drying.[196] Curing follows drying and entails storing trimmed buds in airtight glass jars at 18-21°C and around 58-62% RH to facilitate biochemical changes that enhance aroma, flavor, and smoothness.[193] Jars are filled to 75% capacity and "burped" by opening for 15-30 minutes daily during the first 1-2 weeks to release excess moisture and gases, then less frequently for 2-4 weeks or longer, allowing chlorophyll to break down and cannabinoids to stabilize without significant decarboxylation.[192] Empirical data indicate curing increases moisture slightly (3-13%) and preserves terpene profiles better than uncured storage, though it does not substantially alter cannabinoid content if drying was optimal.[197] Improper curing, such as excessive humidity, promotes Aspergillus contamination, underscoring the need for humidity packs or desiccants to maintain water activity below 0.65 aw.[198] Long-term storage prioritizes minimizing exposure to light, oxygen, heat, and humidity to prevent oxidation of THC to CBN and terpene evaporation, which can degrade potency by 10-20% over months.[199] Sealed mason jars or vacuum-sealed bags in a cool (4-10°C), dark environment extend shelf life to 6-12 months for flower, with freezing at -18°C preserving quality for years by halting enzymatic activity, though repeated freeze-thaw cycles should be avoided.[193] Studies confirm that storage under nitrogen flush or low oxygen reduces cannabinoid loss compared to ambient air, with minimal changes observed at 10-15% moisture and <60% RH.[192] For commercial operations, monitoring with hygrometers and periodic testing for potency and contaminants ensures compliance with quality standards.[200]Technological Advancements
Automation and Precision Agriculture
Automation in cannabis cultivation encompasses robotic systems and software-driven processes that handle repetitive tasks such as planting, irrigation, and harvesting, thereby reducing labor dependency and minimizing human error in controlled environments.[201] Automated irrigation systems deliver precise volumes of water and nutrients based on real-time soil moisture data, while robotic trimmers process harvested material at rates exceeding manual capabilities, with some models handling up to 1,000 grams per hour.[202] These technologies have been adopted in large-scale indoor facilities since 2020, driven by rising labor costs and scalability needs in legalized markets.[203] Precision agriculture techniques integrate sensors, IoT devices, and AI analytics to optimize inputs like light, fertilizers, and CO2 levels tailored to individual plants or zones within a grow operation.[204] For instance, multispectral cameras and machine learning algorithms detect early signs of nutrient deficiencies or pest infestations by analyzing leaf reflectance patterns, enabling targeted interventions that can increase yields by 10-20% compared to uniform application methods.[205] In hydroponic setups, precision fertigation systems adjust pH and EC levels dynamically, using data from embedded probes to prevent over-fertilization, which affects up to 30% of novice grows.[206] AI-driven crop steering models, implemented in commercial operations by 2024, forecast growth trajectories and recommend adjustments to vegetative or flowering cycles based on historical datasets from thousands of plants.[207] Vertical farming integrations further advance these methods by stacking automated layers with centralized climate controls, where AI optimizes airflow and humidity gradients across tiers to achieve uniform cannabinoid profiles.[208] Empirical trials in 2025 reported energy savings of 25% through predictive algorithms that preemptively adjust HVAC systems, though adoption remains limited by high upfront costs averaging $500,000 per modular unit.[209] Challenges include data silos across vendors and the need for validated models, as uncalibrated AI can amplify errors in strain-specific responses, underscoring the importance of empirical validation over vendor claims.[210] Overall, these advancements shift cultivation from artisanal practices to data-centric operations, with projections for 40% of U.S. licensed grows incorporating automation by 2027.[211]Lighting and Vertical Farming Innovations
Light-emitting diode (LED) systems have supplanted traditional high-pressure sodium (HPS) lamps in cannabis cultivation due to superior photosynthetic photon efficacy, reaching up to 3.5 micromoles per joule (μmol/J), which minimizes energy consumption and thermal stress on plants.[212] Unlike HPS, LEDs enable precise spectral control, targeting wavelengths that align with cannabis photoreceptors to optimize vegetative growth, flowering, and secondary metabolite production, including cannabinoids like THC.[213] Studies indicate LEDs produce healthier plants with higher THC content compared to HPS, as evidenced by fuller canopy development and reduced electricity demands in commercial operations post-2020.[214] Innovations such as under-canopy supplemental lighting further enhance light penetration in dense canopies, addressing shading issues in high-density setups.[215] Vertical farming innovations integrate multi-tier racking systems with LED arrays to exploit overhead space, achieving yield increases of up to 300% per square foot over horizontal methods through elevated plant density and uniform light distribution.[208][216] These systems, often hydroponic or aeroponic, incorporate automation for precise environmental control, reducing water usage by 95% via recirculating nutrient solutions and minimizing evaporation in enclosed environments.[208] Tiered configurations, including mezzanine walkways, improve operational efficiency and worker safety while maintaining airflow to prevent pathogen buildup.[217] Combined with AI-driven lighting adjustments, vertical setups enable dynamic photoperiod and intensity modulation tailored to growth stages, enhancing overall resource efficiency in urban or constrained facilities since widespread adoption around 2023.[218]