Water pollution (or aquatic pollution) is the contamination of water bodies, with a negative impact on their uses. It is usually a result of human activities. Water bodies include lakes, rivers, oceans, aquifers, reservoirs and groundwater. Water pollution results when contaminants mix with these water bodies. Contaminants can come from one of four main sources. These are sewage discharges, industrial activities, agricultural activities, and urban runoff including stormwater. Water pollution may affect either surface water or groundwater. This form of pollution can lead to many problems. One is the degradation of aquatic ecosystems. Another is spreading water-borne diseases when people use polluted water for drinking or irrigation. Water pollution also reduces the ecosystem services such as drinking water provided by the water resource.

Sources of water pollution are either point sources or non-point sources. Point sources have one identifiable cause, such as a storm drain, a wastewater treatment plant, or an oil spill. Non-point sources are more diffuse. An example is agricultural runoff. Pollution is the result of the cumulative effect over time. Pollution may take many forms. One would is toxic substances such as oil, metals, plastics, pesticides, persistent organic pollutants, and industrial waste products. Another is stressful conditions such as changes of pH, hypoxia or anoxia, increased temperatures, excessive turbidity, or changes of salinity). The introduction of pathogenic organisms is another. Contaminants may include organic and inorganic substances. A common cause of thermal pollution is the use of water as a coolant by power plants and industrial manufacturers.

Control of water pollution requires appropriate infrastructure and management plans as well as legislation. Technology solutions can include improving sanitation, sewage treatment, industrial wastewater treatment, agricultural wastewater treatment, erosion control, sediment control and control of urban runoff (including stormwater management).

A practical definition of water pollution is: "Water pollution is the addition of substances or energy forms that directly or indirectly alter the nature of the water body in such a manner that negatively affects its legitimate uses." Water is typically referred to as polluted when it is impaired by anthropogenic contaminants. Due to these contaminants, it either no longer supports a certain human use, such as drinking water, or undergoes a marked shift in its ability to support its biotic communities, such as fish.

The following compounds can all reach water bodies via raw sewage or even treated sewage discharges:

Inadequately treated wastewater can convey nutrients, pathogens, heterogenous suspended solids and organic fecal matter.

Bacteria, viruses, protozoans and parasitic worms are examples of pathogens that can be found in wastewater. In practice, indicator organisms are used to investigate pathogenic pollution of water because the detection of pathogenic organisms in water sample is difficult and costly, because of their low concentrations. The indicators (bacterial indicator) of fecal contamination of water samples most commonly used are total coliforms (TC) or fecal coliforms (FC), the latter also referred to as thermotolerant coliforms, such as Escherichia coli.

Pathogens can produce waterborne diseases in either human or animal hosts. Some microorganisms sometimes found in contaminated surface waters that have caused human health problems include Burkholderia pseudomallei, Cryptosporidium parvum, Giardia lamblia, Salmonella, norovirus and other viruses, and parasitic worms including the Schistosoma type.